Skip to main content
PMC home page
Epidemiology and Infection logoLink to Epidemiology and Infection
. 1992 Aug;109(1):59–68.

Restriction endonuclease fingerprinting of genomic DNA of Staphylococcus species of bovine origin.

K R Matthews 1, B M Jayarao 1, S P Oliver 1
PMCID: PMC2272233  PMID: 1499673

Abstract

Fifty-one staphylococcal isolates from mammary secretions of cows with subclinical mastitis were examined by antibiograms and DNA restriction endonuclease fingerprinting (REF). DNA REF differentiated closely related strains of each species isolated from mammary secretions of different mammary glands of the same cow and from the same mammary gland at different periods of the lactation cycle. In addition, REF analysis provided evidence concerning persistence of infection in the same or different mammary gland over different periods of the lactation cycle, and occurrence of infection with similar and dissimilar strains of each Staphylococcus species. Antibiograms were of limited value in differentiating closely related strains. The ease by which REF analysis can be performed together with the reproducibility and clarity of REF patterns suggest that this technique is useful for differentiating closely related and unrelated strains of Staphylococcus species isolated from bovine mammary secretions.

Full text

PDF
59

Images in this article

62Fig. 1
on p.62

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  2. Baumgartner A., Nicolet J., Eggimann M. Plasmid profiles of Staphylococcus aureus causing bovine mastitis. J Appl Bacteriol. 1984 Feb;56(1):159–163. doi: 10.1111/j.1365-2672.1984.tb04708.x. [DOI] [PubMed] [Google Scholar]
  3. Bialkowska-Hobrzanska H., Jaskot D., Hammerberg O. Evaluation of restriction endonuclease fingerprinting of chromosomal DNA and plasmid profile analysis for characterization of multiresistant coagulase-negative staphylococci in bacteremic neonates. J Clin Microbiol. 1990 Feb;28(2):269–275. doi: 10.1128/jcm.28.2.269-275.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burnie J. P., Lee W. A comparison of DNA and immunoblot fingerprinting of the SII biotype of coagulase negative staphylococci. Epidemiol Infect. 1988 Oct;101(2):203–212. doi: 10.1017/s095026880005411x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carroll P. J., Francis P. G. The basic phage set for typing bovine staphylococci. J Hyg (Lond) 1985 Dec;95(3):665–669. doi: 10.1017/s0022172400060769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gershman M., Hunter J. A., Harmon R. J., Wilson R. A., Markowsky G. Phage typing set for differentiating Staphylococcus epidermidis. Can J Microbiol. 1988 Dec;34(12):1358–1361. doi: 10.1139/m88-240. [DOI] [PubMed] [Google Scholar]
  7. Hill A. W., Leigh J. A. DNA fingerprinting of Streptococcus uberis: a useful tool for epidemiology of bovine mastitis. Epidemiol Infect. 1989 Aug;103(1):165–171. doi: 10.1017/s0950268800030466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jayarao B. M., Oliver S. P., Tagg J. R., Matthews K. R. Genotypic and phenotypic analysis of Streptococcus uberis isolated from bovine mammary secretions. Epidemiol Infect. 1991 Dec;107(3):543–555. doi: 10.1017/s0950268800049244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Marshall R. B., Winter P. J., Cooper B. S., Robinson A. J. Subspecies differentiation of Moraxella bovis by restriction endonuclease DNA analysis (BRENDA). N Z Vet J. 1985 May;33(5):67–70. doi: 10.1080/00480169.1985.35167. [DOI] [PubMed] [Google Scholar]
  10. Matthews K. R., Oliver S. P., King S. H. Comparison of Vitek Gram-Positive Identification system with API Staph-Trac system for species identification of staphylococci of bovine origin. J Clin Microbiol. 1990 Jul;28(7):1649–1651. doi: 10.1128/jcm.28.7.1649-1651.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Murray B. E., Singh K. V., Heath J. D., Sharma B. R., Weinstock G. M. Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites. J Clin Microbiol. 1990 Sep;28(9):2059–2063. doi: 10.1128/jcm.28.9.2059-2063.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Patterson J. E., Patterson T. F., Farrel P., Hierholzer W. J., Jr, Zervos M. J. Evaluation of restriction endonuclease analysis as an epidemiologic typing system for Branhamella catarrhalis. J Clin Microbiol. 1989 May;27(5):944–946. doi: 10.1128/jcm.27.5.944-946.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Epidemiology and Infection are provided here courtesy of Cambridge University Press

RESOURCES