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. 1998 Jun 9;95(12):7018–7023. doi: 10.1073/pnas.95.12.7018

Figure 2.

Figure 2

Purification profiles of CBEGF and CBFGF on SDS/PAGE. Samples at each purification step for CBEGF (lanes 2–5) and CBFGF (lanes 6–9), and the final preparation of human recombinant bFGF (lane 10) were electrophoretically separated in a SDS/13% polyacrylamide gel under reducing conditions and then stained with Coomassie brilliant blue R-250. Lane 1, molecular weight markers; lane 2, E. coli BL21/pCHC302-EGF crude extract; lanes 3 and 7, eluate from glutathione-Sepharose; lanes 4 and 8, thrombin digest of the eluate from glutathione-Sepharose; lane 5, eluate from Resource Q (purified CBEGF); lane 6, E. coli BL21/pCHC302-bFGF crude extract; lane 9, eluate from heparin-Sepharose (purified CBFGF); lane 10, purified human recombinant bFGF.