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. Author manuscript; available in PMC: 2009 Feb 1.

Published in final edited form as: Methods. 2008 Feb;44(2):74–80. doi: 10.1016/j.ymeth.2007.08.001

Analysis of k_pep with fMet-tRNA^fMet in the P site and [3’-³²P] Ala-tRNA^Ala in the A site. S1 nuclease digestion of reaction mix results in free [³²P] AMP, [³²P] Ala-AMP, and [³²P] fMet-Ala-AMP. Kinetic data is shown as digestion products developed on a PEI cellulose TLC plate in glacial acetic acid/1 M NH₄Cl/water (5:10:85) and graphically to give k_pep of 2.3 s⁻¹.