Figure 1.

Generation of ASKO mice. A–D, Schematic representation of the STAT3 targeting vector (A), the endogenous STAT3 allele before (WT allele) and after (targeted allele) homologous recombination (B), the targeted allele after FlpE recombination (functional allele) (C), and the functional allele after Cre-mediated deletion of exon 22 (knockout allele) (D); E, Western blot analysis of STAT3 protein expression in heart, liver, kidney, hypothalamus, WAT, and BAT from WT, HZ, and ASKO mice; F, Western blot analysis of STAT3 protein expression in subcutaneous primary preadipocytes isolated from WT, HZ, and ASKO mice 0, 2, 4, 6, and 8 d after induction of differentiation.