TABLE 3.
Impact on genome stability due to deletions in COMA complex genes
| COMA mutation | Strain background | Sectoring (%) | Chromosome V instability rate (events/cell/generation) | Fold change over parental strain |
|---|---|---|---|---|
| — | Wild type | 0.07 | 7.86E-06 | |
| — | rad9Δ/rad9Δ | 0.27 | 3.37E-05 | |
| mcm21Δ/MCM21 | Wild type | 4.10 | 4.03E-03 | 512.72 |
| mcm21Δ/MCM21 | rad9Δ/rad9Δ | 0.65 | 4.21E-06 | 0.12 |
| mcm21Δ/mcm21Δ | Wild type | 0.00 | 1.94E-02 | 2468.19 |
| mcm21Δ/mcm21Δ | rad9Δ/rad9Δ | 0.00 | 1.87E-02 | 554.90 |
| ctf19Δ/CTF19 | Wild type | 6.80 | 2.54E-04 | 32.32 |
| ctf19Δ/CTF19 | rad9Δ/rad9Δ | 8.60 | 1.64E-04 | 4.87 |
| ctf19Δ/ctf19Δ | Wild type | 0.00 | 1.81E-02 | 2302.80 |
| ctf19Δ/ctf19Δ | rad9Δ/rad9Δ | 0.00 | 1.64E-02 | 486.65 |
| okp1Δ/OKP1 | Wild type | 5.00 | 2.72E-05 | 3.46 |
| okp1Δ/OKP1 | rad9Δ/rad9Δ | 3.33 | 1.29E-06 | 0.04 |
| ame1Δ/AME1 | Wild type | 6.60 | 1.47E-05 | 1.87 |
| ame1Δ/AME1 | rad9Δ/rad9Δ | 6.60 | 3.08E-05 | 0.91 |
Sectoring and chromosome V instability rates are shown for heterozygous and homozygous deletion strains for the four members of the COMA complex. Sectoring is shown as a percentage of the total number of sectors in ∼500 colonies. Chromosome V instability rate (events/cell/generation) as measured by conversion to canavanine-resistant cells was estimated via fluctuation analysis. The fold change in the chromosome V instability rate for the indicated strain compared to the respective parental strain rate is shown in column 4. OKP1 and AME1 are essential genes and data are available only for heterozygous strains.