Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1995 Jan;33(1):209–211. doi: 10.1128/jcm.33.1.209-211.1995

Serological reactivity and viral genotypes in hepatitis C virus infection.

F Maggi 1, M L Vatteroni 1, M Pistello 1, C M Avio 1, N Cecconi 1, F Panicucci 1, M Bendinelli 1
PMCID: PMC227910  PMID: 7535312

Abstract

Patients infected with hepatitis C virus (HCV) were examined with four commercial HCV immunoblotting assays and for anti-GOR antibody to ascertain whether serological findings varied with the genotype of the infecting virus. The results indicate that patients infected with different HCV genotypes tend to show different immunoblotting profiles, mainly due to a low prevalence of antibodies to the viral region NS4 in patients infected with genotypes III and IV. Differences were more evident with second- than with third-generation assays. Patients infected with genotype IV exhibited a lower prevalence of anti-GOR antibody than patients infected with other genotypes.

Full Text

The Full Text of this article is available as a PDF (145.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alonso C., Qu D., Lamelin J. P., de Sanjosé S., Vitvitski L., Li J., Berby F., Lambert V., Cortey M. L., Trépo C. Serological responses to different genotypes of hepatitis C virus in France. J Clin Microbiol. 1994 Jan;32(1):211–212. doi: 10.1128/jcm.32.1.211-212.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cha T. A., Beall E., Irvine B., Kolberg J., Chien D., Kuo G., Urdea M. S. At least five related, but distinct, hepatitis C viral genotypes exist. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7144–7148. doi: 10.1073/pnas.89.15.7144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chan S. W., McOmish F., Holmes E. C., Dow B., Peutherer J. F., Follett E., Yap P. L., Simmonds P. Analysis of a new hepatitis C virus type and its phylogenetic relationship to existing variants. J Gen Virol. 1992 May;73(Pt 5):1131–1141. doi: 10.1099/0022-1317-73-5-1131. [DOI] [PubMed] [Google Scholar]
  4. Chan S. W., Simmonds P., McOmish F., Yap P. L., Mitchell R., Dow B., Follett E. Serological responses to infection with three different types of hepatitis C virus. Lancet. 1991 Nov 30;338(8779):1391–1391. doi: 10.1016/0140-6736(91)92265-4. [DOI] [PubMed] [Google Scholar]
  5. Hosein B., Fang C. T., Popovsky M. A., Ye J., Zhang M., Wang C. Y. Improved serodiagnosis of hepatitis C virus infection with synthetic peptide antigen from capsid protein. Proc Natl Acad Sci U S A. 1991 May 1;88(9):3647–3651. doi: 10.1073/pnas.88.9.3647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kuo G., Choo Q. L., Alter H. J., Gitnick G. L., Redeker A. G., Purcell R. H., Miyamura T., Dienstag J. L., Alter M. J., Stevens C. E. An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science. 1989 Apr 21;244(4902):362–364. doi: 10.1126/science.2496467. [DOI] [PubMed] [Google Scholar]
  7. McOmish F., Yap P. L., Dow B. C., Follett E. A., Seed C., Keller A. J., Cobain T. J., Krusius T., Kolho E., Naukkarinen R. Geographical distribution of hepatitis C virus genotypes in blood donors: an international collaborative survey. J Clin Microbiol. 1994 Apr;32(4):884–892. doi: 10.1128/jcm.32.4.884-892.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mehta S. U., Mishiro S., Sekiguchi K., Leung T., Dawson G. J., Pendy L. M., Peterson D. A., Devare S. G. Immune response to GOR, a marker for non-A, non-B hepatitis and its correlation with hepatitis C virus infection. J Clin Immunol. 1992 May;12(3):178–184. doi: 10.1007/BF00918086. [DOI] [PubMed] [Google Scholar]
  9. Mishiro S., Hoshi Y., Takeda K., Yoshikawa A., Gotanda T., Takahashi K., Akahane Y., Yoshizawa H., Okamoto H., Tsuda F. Non-A, non-B hepatitis specific antibodies directed at host-derived epitope: implication for an autoimmune process. Lancet. 1990 Dec 8;336(8728):1400–1403. doi: 10.1016/0140-6736(90)93101-t. [DOI] [PubMed] [Google Scholar]
  10. Mishiro S., Takeda K., Hoshi Y., Yoshikawa A., Gotanda T., Itoh Y. An autoantibody cross-reactive to hepatitis C virus core and a host nuclear antigen. Autoimmunity. 1991;10(4):269–273. doi: 10.3109/08916939109001900. [DOI] [PubMed] [Google Scholar]
  11. Muraiso K., Hijikata M., Ohkoshi S., Cho M. J., Kikuchi M., Kato N., Shimotohno K. A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus. Biochem Biophys Res Commun. 1990 Oct 30;172(2):511–516. doi: 10.1016/0006-291x(90)90702-o. [DOI] [PubMed] [Google Scholar]
  12. Okamoto H., Sugiyama Y., Okada S., Kurai K., Akahane Y., Sugai Y., Tanaka T., Sato K., Tsuda F., Miyakawa Y. Typing hepatitis C virus by polymerase chain reaction with type-specific primers: application to clinical surveys and tracing infectious sources. J Gen Virol. 1992 Mar;73(Pt 3):673–679. doi: 10.1099/0022-1317-73-3-673. [DOI] [PubMed] [Google Scholar]
  13. Okamoto H., Tokita H., Sakamoto M., Horikita M., Kojima M., Iizuka H., Mishiro S. Characterization of the genomic sequence of type V (or 3a) hepatitis C virus isolates and PCR primers for specific detection. J Gen Virol. 1993 Nov;74(Pt 11):2385–2390. doi: 10.1099/0022-1317-74-11-2385. [DOI] [PubMed] [Google Scholar]
  14. Pistello M., Maggi F., Vatteroni L., Cecconi N., Panicucci F., Bresci G. P., Gambardella L., Taddei M., Bionda A., Tuoni M. Prevalence of hepatitis C virus genotypes in Italy. J Clin Microbiol. 1994 Jan;32(1):232–234. doi: 10.1128/jcm.32.1.232-234.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pozzato G., Moretti M., Franzin F., Crocè L. S., Tiribelli C., Masayu T., Kaneko S., Unoura M., Kobayashi K. Severity of liver disease with different hepatitis C viral clones. Lancet. 1991 Aug 24;338(8765):509–509. doi: 10.1016/0140-6736(91)90578-d. [DOI] [PubMed] [Google Scholar]
  16. Simmonds P., Rose K. A., Graham S., Chan S. W., McOmish F., Dow B. C., Follett E. A., Yap P. L., Marsden H. Mapping of serotype-specific, immunodominant epitopes in the NS-4 region of hepatitis C virus (HCV): use of type-specific peptides to serologically differentiate infections with HCV types 1, 2, and 3. J Clin Microbiol. 1993 Jun;31(6):1493–1503. doi: 10.1128/jcm.31.6.1493-1503.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vatteroni M., Pistello M., Maggi F., Cecconi N., Panicucci F., Bendinelli M. Hepatitis C virus serological and polymerase chain reactions in human immunodeficiency virus-positive and -negative patients. Clin Diagn Virol. 1994 Feb;2(1):7–16. doi: 10.1016/0928-0197(94)90031-0. [DOI] [PubMed] [Google Scholar]
  18. Yoshioka K., Kakumu S., Wakita T., Ishikawa T., Itoh Y., Takayanagi M., Higashi Y., Shibata M., Morishima T. Detection of hepatitis C virus by polymerase chain reaction and response to interferon-alpha therapy: relationship to genotypes of hepatitis C virus. Hepatology. 1992 Aug;16(2):293–299. doi: 10.1002/hep.1840160203. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES