Abstract
Quality control guidelines for tilmicosin, a novel veterinary-use-only macrolide, were developed in a multi-laboratory study according to established National Committee for Clinical Laboratory Standards (NCCLS) procedures (M23-T2). Tilmicosin was incorporated into Sensititre plates for broth microdilution endpoint testing and into two lots of 15-micrograms disks for Kirby-Bauer agar disk diffusion testing. One common lot and five unique lots of Mueller-Hinton media were used. (Broth was cation adjusted, and agar was supplemented with 5% defibrinated sheep blood.) Bacteria used for reference strains included Pasteurella haemolytica 128K, Pasteurella multocida ATCC 43137, and Staphylococcus aureus ATCC 29213 (microdilution) and ATCC 25923 (disk). Replicate tests were conducted. Disk diffusion and broth microdilution quality control ranges are proposed.
Full Text
The Full Text of this article is available as a PDF (186.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bale M. J., Jones R. N. Quality control guidelines for cefdinir, cefepime, cefetamet, cefmetazole, cefpodoxime, cefprozil, and clinafloxacin (CI-960) for various National Committee for Clinical Laboratory Standards susceptibility testing methods. Quality Control Study Group. J Clin Microbiol. 1993 Sep;31(9):2538–2540. doi: 10.1128/jcm.31.9.2538-2540.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barry A. L., Fuchs P. C., Jones R. N. Statistical criteria for selecting quality control limits for broth microdilution susceptibility tests with 39 different antimicrobial agents. Collaborative Antimicrobial Susceptibility Testing Group. Diagn Microbiol Infect Dis. 1989 Sep-Oct;12(5):413–420. doi: 10.1016/0732-8893(89)90112-0. [DOI] [PubMed] [Google Scholar]
- Gavan T. L., Jones R. N., Barry A. L. Evaluation of the Sensititre system for quantitative antimicrobial drug susceptibility testing: a collaborative study. Antimicrob Agents Chemother. 1980 Mar;17(3):464–469. doi: 10.1128/aac.17.3.464. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gavan T. L., Jones R. N., Barry A. L., Fuchs P. C., Gerlach E. H., Matsen J. M., Reller L. B., Thornsberry C., Thrupp L. D. Quality control limits for ampicillin, carbenicillin, mezlocillin, and piperacillin disk diffusion susceptibility tests: a collaborative study. J Clin Microbiol. 1981 Jul;14(1):67–72. doi: 10.1128/jcm.14.1.67-72.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gorham P. E., Carroll L. H., McAskill J. W., Watkins L. E., Ose E. E., Tonkinson L. V., Merrill J. K. Tilmicosin as a single injection treatment for respiratory disease of feedlot cattle. Can Vet J. 1990 Dec;31(12):826–829. [PMC free article] [PubMed] [Google Scholar]
- Laven R., Andrews A. H. Long-acting antibiotic formulations in the treatment of calf pneumonia: a comparative study of tilmicosin and oxytetracycline. Vet Rec. 1991 Aug 10;129(6):109–111. doi: 10.1136/vr.129.6.109. [DOI] [PubMed] [Google Scholar]
- Ose E. E. In vitro antibacterial properties of EL-870, a new semi-synthetic macrolide antibiotic. J Antibiot (Tokyo) 1987 Feb;40(2):190–194. doi: 10.7164/antibiotics.40.190. [DOI] [PubMed] [Google Scholar]
- Ose E. E., Tonkinson L. V. Single-dose treatment of neonatal calf pneumonia with the new macrolide antibiotic tilmicosin. Vet Rec. 1988 Oct 1;123(14):367–369. doi: 10.1136/vr.123.14.367. [DOI] [PubMed] [Google Scholar]
- Watts J. L., Yancey R. J., Jr Identification of veterinary pathogens by use of commercial identification systems and new trends in antimicrobial susceptibility testing of veterinary pathogens. Clin Microbiol Rev. 1994 Jul;7(3):346–356. doi: 10.1128/cmr.7.3.346. [DOI] [PMC free article] [PubMed] [Google Scholar]