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. 1995 Feb;33(2):376–380. doi: 10.1128/jcm.33.2.376-380.1995

Molecular population genetic analysis of Staphylococcus aureus recovered from cows.

V Kapur 1, W M Sischo 1, R S Greer 1, T S Whittam 1, J M Musser 1
PMCID: PMC227951  PMID: 7714195

Abstract

Staphylococcus aureus is one of the most common causes of bovine mastitis. To estimate genetic relationships among S. aureus strains recovered from cows, 357 isolates from milk samples from worldwide localities were examined for electrophoretic variation at 13 metabolic-enzyme loci. Thirty-nine electrophoretic types which represented distinctive multilocus enzyme genotypes were identified, and nearly 90% of all isolates were assigned to one of eight clones. Genetic heterogeneity was found among organisms recovered from dairy herds from which multiple isolates were obtained, indicating that the S. aureus population in a single herd can be multiclonal. Although humans and cows shared 7 of the 39 S. aureus clones, each clone was predominantly associated with one of these host species. These results are consistent with the concept of host specialization among S. aureus clones and imply that successful transfer of bacteria between humans and cows is limited.

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Selected References

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  1. Aguilar B., Iturralde M., Baselga R., Amorena B. An efficient microtest to study adherence of bacteria to mammalian cells. FEMS Microbiol Lett. 1992 Jan 1;69(2):161–164. doi: 10.1016/0378-1097(92)90621-t. [DOI] [PubMed] [Google Scholar]
  2. Archer G. L., Niemeyer D. M., Thanassi J. A., Pucci M. J. Dissemination among staphylococci of DNA sequences associated with methicillin resistance. Antimicrob Agents Chemother. 1994 Mar;38(3):447–454. doi: 10.1128/aac.38.3.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baumgartner A., Nicolet J., Eggimann M. Plasmid profiles of Staphylococcus aureus causing bovine mastitis. J Appl Bacteriol. 1984 Feb;56(1):159–163. doi: 10.1111/j.1365-2672.1984.tb04708.x. [DOI] [PubMed] [Google Scholar]
  4. Boyd E. F., Nelson K., Wang F. S., Whittam T. S., Selander R. K. Molecular genetic basis of allelic polymorphism in malate dehydrogenase (mdh) in natural populations of Escherichia coli and Salmonella enterica. Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1280–1284. doi: 10.1073/pnas.91.4.1280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carroll P. J., Francis P. G. The basic phage set for typing bovine staphylococci. J Hyg (Lond) 1985 Dec;95(3):665–669. doi: 10.1017/s0022172400060769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dykhuizen D. E., de Framond J., Hartl D. L. Selective neutrality of glucose-6-phosphate dehydrogenase allozymes in Escherichia coli. Mol Biol Evol. 1984 Feb;1(2):162–170. doi: 10.1093/oxfordjournals.molbev.a040309. [DOI] [PubMed] [Google Scholar]
  7. Dykhuizen D., Hartl D. L. Selective neutrality of 6PGD allozymes in E. coli and the effects of genetic background. Genetics. 1980 Dec;96(4):801–817. doi: 10.1093/genetics/96.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Foster T. J. Potential for vaccination against infections caused by Staphylococcus aureus. Vaccine. 1991 Apr;9(4):221–227. doi: 10.1016/0264-410x(91)90103-d. [DOI] [PubMed] [Google Scholar]
  9. Fox L. K., Gershman M., Hancock D. D., Hutton C. T. Fomites and reservoirs of Staphylococcus aureus causing intramammary infections as determined by phage typing: the effect of milking time hygiene practices. Cornell Vet. 1991 Apr;81(2):183–193. [PubMed] [Google Scholar]
  10. Hartl D. L., Dykhuizen D. E. The population genetics of Escherichia coli. Annu Rev Genet. 1984;18:31–68. doi: 10.1146/annurev.ge.18.120184.000335. [DOI] [PubMed] [Google Scholar]
  11. Hinckley L. S., Benson R. H., Post J. E., DeCloux J. C. Antibiotic susceptibility profiles for mastitis treatment. J Am Vet Med Assoc. 1985 Oct 1;187(7):709–711. [PubMed] [Google Scholar]
  12. Jayarao B. M., Schilling E. E., Oliver S. P. Genomic deoxyribonucleic acid restriction fragment length polymorphism of Streptococcus uberis: evidence of clonal diversity. J Dairy Sci. 1993 Feb;76(2):468–474. doi: 10.3168/jds.S0022-0302(93)77367-1. [DOI] [PubMed] [Google Scholar]
  13. Kenny K., Reiser R. F., Bastida-Corcuera F. D., Norcross N. L. Production of enterotoxins and toxic shock syndrome toxin by bovine mammary isolates of Staphylococcus aureus. J Clin Microbiol. 1993 Mar;31(3):706–707. doi: 10.1128/jcm.31.3.706-707.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kreiswirth B., Kornblum J., Arbeit R. D., Eisner W., Maslow J. N., McGeer A., Low D. E., Novick R. P. Evidence for a clonal origin of methicillin resistance in Staphylococcus aureus. Science. 1993 Jan 8;259(5092):227–230. doi: 10.1126/science.8093647. [DOI] [PubMed] [Google Scholar]
  15. Lee P. K., Kreiswirth B. N., Deringer J. R., Projan S. J., Eisner W., Smith B. L., Carlson E., Novick R. P., Schlievert P. M. Nucleotide sequences and biologic properties of toxic shock syndrome toxin 1 from ovine- and bovine-associated Staphylococcus aureus. J Infect Dis. 1992 Jun;165(6):1056–1063. doi: 10.1093/infdis/165.6.1056. [DOI] [PubMed] [Google Scholar]
  16. Lopes C. A., Moreno G., Curi P. R., Gottschalk A. F., Modolo J. R., Horacio A., Corrêa A., Pavan C. Characteristics of Staphylococcus aureus from subclinical bovine mastitis in Brazil. Br Vet J. 1990 Sep-Oct;146(5):443–448. doi: 10.1016/0007-1935(90)90033-y. [DOI] [PubMed] [Google Scholar]
  17. Mackie D. P., Logan E. F., Pollock D. A., Rodgers S. P. Antibiotic sensitivity of bovine staphylococcal and coliform mastitis isolates over four years. Vet Rec. 1988 Nov 12;123(20):515–517. doi: 10.1136/vr.123.20.515. [DOI] [PubMed] [Google Scholar]
  18. Marr J. C., Lyon J. D., Roberson J. R., Lupher M., Davis W. C., Bohach G. A. Characterization of novel type C staphylococcal enterotoxins: biological and evolutionary implications. Infect Immun. 1993 Oct;61(10):4254–4262. doi: 10.1128/iai.61.10.4254-4262.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Matos J. S., White D. G., Harmon R. J., Langlois B. E. Isolation of Staphylococcus aureus from sites other than the lactating mammary gland. J Dairy Sci. 1991 May;74(5):1544–1549. doi: 10.3168/jds.S0022-0302(91)78315-X. [DOI] [PubMed] [Google Scholar]
  20. Matthews K. R., Jayarao B. M., Oliver S. P. Plasmid pattern analysis of Staphylococcus species isolated from bovine mammary secretions. J Dairy Sci. 1992 Dec;75(12):3318–3323. doi: 10.3168/jds.S0022-0302(92)78107-7. [DOI] [PubMed] [Google Scholar]
  21. Matthews K. R., Jayarao B. M., Oliver S. P. Restriction endonuclease fingerprinting of genomic DNA of Staphylococcus species of bovine origin. Epidemiol Infect. 1992 Aug;109(1):59–68. [PMC free article] [PubMed] [Google Scholar]
  22. Miles H., Lesser W., Sears P. The economic implications of bioengineered mastitis control. J Dairy Sci. 1992 Feb;75(2):596–605. doi: 10.3168/jds.S0022-0302(92)77797-2. [DOI] [PubMed] [Google Scholar]
  23. Musser J. M., Kapur V. Clonal analysis of methicillin-resistant Staphylococcus aureus strains from intercontinental sources: association of the mec gene with divergent phylogenetic lineages implies dissemination by horizontal transfer and recombination. J Clin Microbiol. 1992 Aug;30(8):2058–2063. doi: 10.1128/jcm.30.8.2058-2063.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Musser J. M., Schlievert P. M., Chow A. W., Ewan P., Kreiswirth B. N., Rosdahl V. T., Naidu A. S., Witte W., Selander R. K. A single clone of Staphylococcus aureus causes the majority of cases of toxic shock syndrome. Proc Natl Acad Sci U S A. 1990 Jan;87(1):225–229. doi: 10.1073/pnas.87.1.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Naidu A. S., Forsgren A., Kalfas S., Watts J. L., Fournier J. M. Comparison between lactoferrin and subepithelial matrix protein binding in Staphylococcus aureus associated with bovine mastitis. J Dairy Sci. 1991 Oct;74(10):3353–3359. doi: 10.3168/jds.S0022-0302(91)78524-X. [DOI] [PubMed] [Google Scholar]
  26. Nelson K., Selander R. K. Evolutionary genetics of the proline permease gene (putP) and the control region of the proline utilization operon in populations of Salmonella and Escherichia coli. J Bacteriol. 1992 Nov;174(21):6886–6895. doi: 10.1128/jb.174.21.6886-6895.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Nelson K., Whittam T. S., Selander R. K. Nucleotide polymorphism and evolution in the glyceraldehyde-3-phosphate dehydrogenase gene (gapA) in natural populations of Salmonella and Escherichia coli. Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6667–6671. doi: 10.1073/pnas.88.15.6667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Owens W. E., Watts J. L. Antimicrobial susceptibility and beta-lactamase testing of staphylococci isolated from dairy herds. J Dairy Sci. 1988 Jul;71(7):1934–1939. doi: 10.3168/jds.S0022-0302(88)79763-5. [DOI] [PubMed] [Google Scholar]
  29. Schukken Y. H., Mallard B. A., Dekkers J. C., Leslie K. E., Stear M. J. Genetic impact on the risk of intramammary infection following Staphylococcus aureus challenge. J Dairy Sci. 1994 Feb;77(2):639–647. doi: 10.3168/jds.S0022-0302(94)76994-0. [DOI] [PubMed] [Google Scholar]
  30. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986 May;51(5):873–884. doi: 10.1128/aem.51.5.873-884.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Sischo W. M., Heider L. E., Miller G. Y., Moore D. A. Prevalence of contagious pathogens of bovine mastitis and use of mastitis control practices. J Am Vet Med Assoc. 1993 Feb 15;202(4):595–600. [PubMed] [Google Scholar]
  32. Smith J. M., Smith N. H., O'Rourke M., Spratt B. G. How clonal are bacteria? Proc Natl Acad Sci U S A. 1993 May 15;90(10):4384–4388. doi: 10.1073/pnas.90.10.4384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sutra L., Poutrel B. Virulence factors involved in the pathogenesis of bovine intramammary infections due to Staphylococcus aureus. J Med Microbiol. 1994 Feb;40(2):79–89. doi: 10.1099/00222615-40-2-79. [DOI] [PubMed] [Google Scholar]
  34. Trinidad P., Nickerson S. C., Alley T. K. Prevalence of intramammary infection and teat canal colonization in unbred and primigravid dairy heifers. J Dairy Sci. 1990 Jan;73(1):107–114. doi: 10.3168/jds.S0022-0302(90)78652-3. [DOI] [PubMed] [Google Scholar]
  35. Trinidad P., Nickerson S. C., Luther D. G. Antimicrobial susceptibilities of staphylococcal species isolated from mammary glands of unbred and primigravid dairy heifers. J Dairy Sci. 1990 Feb;73(2):357–362. doi: 10.3168/jds.S0022-0302(90)78681-X. [DOI] [PubMed] [Google Scholar]
  36. Watson D. L. Staphylococcal mastitis vaccine. Vaccine. 1992;10(5):359–359. doi: 10.1016/0264-410x(92)90387-y. [DOI] [PubMed] [Google Scholar]

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