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. 2002 Jun;13(2):49–55.

Automated, rapid solid-phase proteolytic cleavage and sample preparation for proteomics

P Metalnikov, P O'Donnel, G Vassilovski, K Ashman
PMCID: PMC2279853  PMID: 19498966

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aguilar M. I., Clayton D. J., Holt P., Kronina V., Boysen R. I., Purcell A. W., Hearn M. T. RP-HPLC binding domains of proteins. Anal Chem. 1998 Dec 1;70(23):5010–5018. doi: 10.1021/ac980473c. [DOI] [PubMed] [Google Scholar]
  2. Baldwin M. A., Medzihradszky K. F., Lock C. M., Fisher B., Settineri T. A., Burlingame A. L. Matrix-assisted laser desorption/ionization coupled with quadrupole/orthogonal acceleration time-of-flight mass spectrometry for protein discovery, identification, and structural analysis. Anal Chem. 2001 Apr 15;73(8):1707–1720. doi: 10.1021/ac0011080. [DOI] [PubMed] [Google Scholar]
  3. Chernushevich I. V., Loboda A. V., Thomson B. A. An introduction to quadrupole-time-of-flight mass spectrometry. J Mass Spectrom. 2001 Aug;36(8):849–865. doi: 10.1002/jms.207. [DOI] [PubMed] [Google Scholar]
  4. Doucette A., Craft D., Li L. Protein concentration and enzyme digestion on microbeads for MALDI-TOF peptides mass mapping of proteins from dilute solutions. Anal Chem. 2000 Jul 15;72(14):3355–3362. doi: 10.1021/ac000176j. [DOI] [PubMed] [Google Scholar]
  5. Ekström S., Onnerfjord P., Nilsson J., Bengtsson M., Laurell T., Marko-Varga G. Integrated microanalytical technology enabling rapid and automated protein identification. Anal Chem. 2000 Jan 15;72(2):286–293. doi: 10.1021/ac990731l. [DOI] [PubMed] [Google Scholar]
  6. Gao J., Xu J., Locascio L. E., Lee C. S. Integrated microfluidic system enabling protein digestion, peptide separation, and protein identification. Anal Chem. 2001 Jun 1;73(11):2648–2655. doi: 10.1021/ac001126h. [DOI] [PubMed] [Google Scholar]
  7. Lee C. H., McComb M. E., Bromirski M., Jilkine A., Ens W., Standing K. G., Perreault H. On-membrane digestion of beta-casein for determination of phosphorylation sites by matrix-assisted laser desorption/ionization quadrupole/time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 2001;15(3):191–202. doi: 10.1002/1097-0231(20010215)15:3<191::AID-RCM209>3.0.CO;2-N. [DOI] [PubMed] [Google Scholar]
  8. Loboda AV, Krutchinsky AN, Bromirski M, Ens W, Standing KG. A tandem quadrupole/time-of-flight mass spectrometer with a matrix-assisted laser desorption/ionization source: design and performance. Rapid Commun Mass Spectrom. 2000;14(12):1047–1057. doi: 10.1002/1097-0231(20000630)14:12<1047::AID-RCM990>3.0.CO;2-E. [DOI] [PubMed] [Google Scholar]
  9. Qian X., Zhou W., Khaledi M. G., Tomer K. B. Direct analysis of the products of sequential cleavages of peptides and proteins affinity-bound to immobilized metal ion beads by matrix-assisted laser desorption/ionization mass spectrometry. Anal Biochem. 1999 Oct 15;274(2):174–180. doi: 10.1006/abio.1999.4268. [DOI] [PubMed] [Google Scholar]
  10. Quadroni M., James P. Proteomics and automation. Electrophoresis. 1999 Apr-May;20(4-5):664–677. doi: 10.1002/(SICI)1522-2683(19990101)20:4/5<664::AID-ELPS664>3.0.CO;2-A. [DOI] [PubMed] [Google Scholar]
  11. Shevchenko A., Loboda A., Shevchenko A., Ens W., Standing K. G. MALDI quadrupole time-of-flight mass spectrometry: a powerful tool for proteomic research. Anal Chem. 2000 May 1;72(9):2132–2141. doi: 10.1021/ac9913659. [DOI] [PubMed] [Google Scholar]
  12. Vecchione G., Casetta B., Santacroce R., Margaglione M. A comprehensive on-line digestion-liquid chromatography/mass spectrometry/collision-induced dissociation mass spectrometry approach for the characterization of human fibrinogen. Rapid Commun Mass Spectrom. 2001;15(16):1383–1390. doi: 10.1002/rcm.355. [DOI] [PubMed] [Google Scholar]
  13. Wang C., Oleschuk R., Ouchen F., Li J., Thibault P., Harrison D. J. Integration of immobilized trypsin bead beds for protein digestion within a microfluidic chip incorporating capillary electrophoresis separations and an electrospray mass spectrometry interface. Rapid Commun Mass Spectrom. 2000;14(15):1377–1383. doi: 10.1002/1097-0231(20000815)14:15<1377::AID-RCM31>3.0.CO;2-2. [DOI] [PubMed] [Google Scholar]
  14. Xue Q., Dunayevskiy Y. M., Foret F., Karger B. L. Integrated multichannel microchip electrospray ionization mass spectrometry: analysis of peptides from on-chip tryptic digestion of melittin. Rapid Commun Mass Spectrom. 1997;11(12):1253–1256. doi: 10.1002/(SICI)1097-0231(199708)11:12<1253::AID-RCM17>3.0.CO;2-6. [DOI] [PubMed] [Google Scholar]

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