Abstract
Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).
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- Clarridge J. E., 3rd, Shawar R. M., Shinnick T. M., Plikaytis B. B. Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory. J Clin Microbiol. 1993 Aug;31(8):2049–2056. doi: 10.1128/jcm.31.8.2049-2056.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eisenach K. D., Cave M. D., Bates J. H., Crawford J. T. Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis. J Infect Dis. 1990 May;161(5):977–981. doi: 10.1093/infdis/161.5.977. [DOI] [PubMed] [Google Scholar]
- Fauville-Dufaux M., Vanfleteren B., De Wit L., Vincke J. P., Van Vooren J. P., Yates M. D., Serruys E., Content J. Rapid detection of tuberculous and non-tuberculous mycobacteria by polymerase chain reaction amplification of a 162 bp DNA fragment from antigen 85. Eur J Clin Microbiol Infect Dis. 1992 Sep;11(9):797–803. doi: 10.1007/BF01960878. [DOI] [PubMed] [Google Scholar]
- Hance A. J., Grandchamp B., Lévy-Frébault V., Lecossier D., Rauzier J., Bocart D., Gicquel B. Detection and identification of mycobacteria by amplification of mycobacterial DNA. Mol Microbiol. 1989 Jul;3(7):843–849. doi: 10.1111/j.1365-2958.1989.tb00233.x. [DOI] [PubMed] [Google Scholar]
- Linton C. J., Jalal H., Leeming J. P., Millar M. R. Rapid discrimination of Mycobacterium tuberculosis strains by random amplified polymorphic DNA analysis. J Clin Microbiol. 1994 Sep;32(9):2169–2174. doi: 10.1128/jcm.32.9.2169-2174.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mantero G., Zonaro A., Albertini A., Bertolo P., Primi D. DNA enzyme immunoassay: general method for detecting products of polymerase chain reaction. Clin Chem. 1991 Mar;37(3):422–429. [PubMed] [Google Scholar]
- Nolte F. S., Metchock B., McGowan J. E., Jr, Edwards A., Okwumabua O., Thurmond C., Mitchell P. S., Plikaytis B., Shinnick T. Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization. J Clin Microbiol. 1993 Jul;31(7):1777–1782. doi: 10.1128/jcm.31.7.1777-1782.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Soini H., Skurnik M., Liippo K., Tala E., Viljanen M. K. Detection and identification of mycobacteria by amplification of a segment of the gene coding for the 32-kilodalton protein. J Clin Microbiol. 1992 Aug;30(8):2025–2028. doi: 10.1128/jcm.30.8.2025-2028.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thierry D., Brisson-Noël A., Vincent-Lévy-Frébault V., Nguyen S., Guesdon J. L., Gicquel B. Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis. J Clin Microbiol. 1990 Dec;28(12):2668–2673. doi: 10.1128/jcm.28.12.2668-2673.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Walker D. A., Taylor I. K., Mitchell D. M., Shaw R. J. Comparison of polymerase chain reaction amplification of two mycobacterial DNA sequences, IS6110 and the 65kDa antigen gene, in the diagnosis of tuberculosis. Thorax. 1992 Sep;47(9):690–694. doi: 10.1136/thx.47.9.690. [DOI] [PMC free article] [PubMed] [Google Scholar]