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. 1995 Apr;33(4):797–801. doi: 10.1128/jcm.33.4.797-801.1995

Typing of human astroviruses from clinical isolates by enzyme immunoassay and nucleotide sequencing.

J S Noel 1, T W Lee 1, J B Kurtz 1, R I Glass 1, S S Monroe 1
PMCID: PMC228043  PMID: 7790440

Abstract

A typing enzyme immunoassay (TYPE-EIA) was used to determine the antigenic types of 64 astrovirus-positive specimens from nine collections from seven countries. Six of the seven known astrovirus types were detected in the collections, with HAstV-1 predominating in all collections for one from the United Kingdom. Selected specimens were analyzed further by reverse transcriptase PCR and nucleotide sequencing of 348 bp within the capsid protein precursor region of the genome. The phylogenetic groupings (genotypes) determined from the sequences were entirely consistent with the antigenic groupings (serotypes) of isolates obtained by using the TYPE-EIA. The genetic variation within genotypes was small compared with the variation between genotypes, allowing unambiguous categorization of all specimens. Although some strains from widely separated geographic areas had identical sequences, in general, within a region most strains of the same type were identical. The TYPE-EIA may help further our understanding of the epidemiology of astrovirus and the possible role of serotype-specific immunity, while further knowledge of sequences could facilitate the development of simpler molecular methods of typing astrovirus strains.

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Selected References

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  1. Appleton H., Higgins P. G. Letter: Viruses and gastroenteritis in infants. Lancet. 1975 Jun 7;1(7919):1297–1297. doi: 10.1016/s0140-6736(75)92581-7. [DOI] [PubMed] [Google Scholar]
  2. Bates P. R., Bailey A. S., Wood D. J., Morris D. J., Couriel J. M. Comparative epidemiology of rotavirus, subgenus F (types 40 and 41) adenovirus and astrovirus gastroenteritis in children. J Med Virol. 1993 Mar;39(3):224–228. doi: 10.1002/jmv.1890390309. [DOI] [PubMed] [Google Scholar]
  3. Cruz J. R., Bartlett A. V., Herrmann J. E., Cáceres P., Blacklow N. R., Cano F. Astrovirus-associated diarrhea among Guatemalan ambulatory rural children. J Clin Microbiol. 1992 May;30(5):1140–1144. doi: 10.1128/jcm.30.5.1140-1144.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dear S., Staden R. A sequence assembly and editing program for efficient management of large projects. Nucleic Acids Res. 1991 Jul 25;19(14):3907–3911. doi: 10.1093/nar/19.14.3907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Greenberg H. B., Matsui S. M. Astroviruses and caliciviruses: emerging enteric pathogens. Infect Agents Dis. 1992 Apr;1(2):71–91. [PubMed] [Google Scholar]
  6. Herrmann J. E., Cubitt W. D., Hudson R. W., Perron-Henry D. M., Oshiro L. S., Blacklow N. R. Immunological characterization of the Marin County strain of astrovirus. Arch Virol. 1990;110(3-4):213–220. doi: 10.1007/BF01311289. [DOI] [PubMed] [Google Scholar]
  7. Herrmann J. E., Hudson R. W., Perron-Henry D. M., Kurtz J. B., Blacklow N. R. Antigenic characterization of cell-cultivated astrovirus serotypes and development of astrovirus-specific monoclonal antibodies. J Infect Dis. 1988 Jul;158(1):182–185. doi: 10.1093/infdis/158.1.182. [DOI] [PubMed] [Google Scholar]
  8. Herrmann J. E., Nowak N. A., Perron-Henry D. M., Hudson R. W., Cubitt W. D., Blacklow N. R. Diagnosis of astrovirus gastroenteritis by antigen detection with monoclonal antibodies. J Infect Dis. 1990 Feb;161(2):226–229. doi: 10.1093/infdis/161.2.226. [DOI] [PubMed] [Google Scholar]
  9. Herrmann J. E., Taylor D. N., Echeverria P., Blacklow N. R. Astroviruses as a cause of gastroenteritis in children. N Engl J Med. 1991 Jun 20;324(25):1757–1760. doi: 10.1056/NEJM199106203242501. [DOI] [PubMed] [Google Scholar]
  10. Hudson R. W., Herrmann J. E., Blacklow N. R. Plaque quantitation and virus neutralization assays for human astroviruses. Arch Virol. 1989;108(1-2):33–38. doi: 10.1007/BF01313740. [DOI] [PubMed] [Google Scholar]
  11. Jiang B., Monroe S. S., Koonin E. V., Stine S. E., Glass R. I. RNA sequence of astrovirus: distinctive genomic organization and a putative retrovirus-like ribosomal frameshifting signal that directs the viral replicase synthesis. Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10539–10543. doi: 10.1073/pnas.90.22.10539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jiang X., Wang J., Graham D. Y., Estes M. K. Detection of Norwalk virus in stool by polymerase chain reaction. J Clin Microbiol. 1992 Oct;30(10):2529–2534. doi: 10.1128/jcm.30.10.2529-2534.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Konno T., Suzuki H., Ishida N., Chiba R., Mochizuki K., Tsunoda A. Astrovirus-associated epidemic gastroenteritis in Japan. J Med Virol. 1982;9(1):11–17. doi: 10.1002/jmv.1890090103. [DOI] [PubMed] [Google Scholar]
  14. Kotloff K. L., Herrmann J. E., Blacklow N. R., Hudson R. W., Wasserman S. S., Morris J. G., Jr, Levine M. M. The frequency of astrovirus as a cause of diarrhea in Baltimore children. Pediatr Infect Dis J. 1992 Jul;11(7):587–589. [PubMed] [Google Scholar]
  15. Kurtz J. B., Lee T. W. Human astrovirus serotypes. Lancet. 1984 Dec 15;2(8416):1405–1405. doi: 10.1016/s0140-6736(84)92101-9. [DOI] [PubMed] [Google Scholar]
  16. Lee T. W., Kurtz J. B. Human astrovirus serotypes. J Hyg (Lond) 1982 Dec;89(3):539–540. doi: 10.1017/s0022172400071114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lee T. W., Kurtz J. B. Prevalence of human astrovirus serotypes in the Oxford region 1976-92, with evidence for two new serotypes. Epidemiol Infect. 1994 Feb;112(1):187–193. doi: 10.1017/s0950268800057551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Leite J. P., Barth O. M., Schatzmayr H. G. Astrovirus in faeces of children with acute gastroenteritis in Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz. 1991 Oct-Dec;86(4):489–490. doi: 10.1590/s0074-02761991000400025. [DOI] [PubMed] [Google Scholar]
  19. Lew J. F., Glass R. I., Petric M., Lebaron C. W., Hammond G. W., Miller S. E., Robinson C., Boutilier J., Riepenhoff-Talty M., Payne C. M. Six-year retrospective surveillance of gastroenteritis viruses identified at ten electron microscopy centers in the United States and Canada. Pediatr Infect Dis J. 1990 Oct;9(10):709–714. doi: 10.1097/00006454-199010000-00005. [DOI] [PubMed] [Google Scholar]
  20. Lew J. F., Moe C. L., Monroe S. S., Allen J. R., Harrison B. M., Forrester B. D., Stine S. E., Woods P. A., Hierholzer J. C., Herrmann J. E. Astrovirus and adenovirus associated with diarrhea in children in day care settings. J Infect Dis. 1991 Oct;164(4):673–678. doi: 10.1093/infdis/164.4.673. [DOI] [PubMed] [Google Scholar]
  21. Lewis T. L., Greenberg H. B., Herrmann J. E., Smith L. S., Matsui S. M. Analysis of astrovirus serotype 1 RNA, identification of the viral RNA-dependent RNA polymerase motif, and expression of a viral structural protein. J Virol. 1994 Jan;68(1):77–83. doi: 10.1128/jvi.68.1.77-83.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Madeley C. R., Cosgrove B. P. Letter: Viruses in infantile gastroenteritis. Lancet. 1975 Jul 19;2(7925):124–124. doi: 10.1016/s0140-6736(75)90020-3. [DOI] [PubMed] [Google Scholar]
  23. Marczinke B., Bloys A. J., Brown T. D., Willcocks M. M., Carter M. J., Brierley I. The human astrovirus RNA-dependent RNA polymerase coding region is expressed by ribosomal frameshifting. J Virol. 1994 Sep;68(9):5588–5595. doi: 10.1128/jvi.68.9.5588-5595.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Matsui S. M., Kim J. P., Greenberg H. B., Young L. M., Smith L. S., Lewis T. L., Herrmann J. E., Blacklow N. R., Dupuis K., Reyes G. R. Cloning and characterization of human astrovirus immunoreactive epitopes. J Virol. 1993 Mar;67(3):1712–1715. doi: 10.1128/jvi.67.3.1712-1715.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Mitchell D. K., Van R., Morrow A. L., Monroe S. S., Glass R. I., Pickering L. K. Outbreaks of astrovirus gastroenteritis in day care centers. J Pediatr. 1993 Nov;123(5):725–732. doi: 10.1016/s0022-3476(05)80846-7. [DOI] [PubMed] [Google Scholar]
  26. Moe C. L., Allen J. R., Monroe S. S., Gary H. E., Jr, Humphrey C. D., Herrmann J. E., Blacklow N. R., Carcamo C., Koch M., Kim K. H. Detection of astrovirus in pediatric stool samples by immunoassay and RNA probe. J Clin Microbiol. 1991 Nov;29(11):2390–2395. doi: 10.1128/jcm.29.11.2390-2395.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Monroe S. S., Glass R. I., Noah N., Flewett T. H., Caul E. O., Ashton C. I., Curry A., Field A. M., Madeley R., Pead P. J. Electron microscopic reporting of gastrointestinal viruses in the United Kingdom, 1985-1987. J Med Virol. 1991 Mar;33(3):193–198. doi: 10.1002/jmv.1890330310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Monroe S. S., Jiang B., Stine S. E., Koopmans M., Glass R. I. Subgenomic RNA sequence of human astrovirus supports classification of Astroviridae as a new family of RNA viruses. J Virol. 1993 Jun;67(6):3611–3614. doi: 10.1128/jvi.67.6.3611-3614.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Monroe S. S., Stine S. E., Gorelkin L., Herrmann J. E., Blacklow N. R., Glass R. I. Temporal synthesis of proteins and RNAs during human astrovirus infection of cultured cells. J Virol. 1991 Feb;65(2):641–648. doi: 10.1128/jvi.65.2.641-648.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Noel J., Cubitt D. Identification of astrovirus serotypes from children treated at the Hospitals for Sick Children, London 1981-93. Epidemiol Infect. 1994 Aug;113(1):153–159. doi: 10.1017/s0950268800051578. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Oishi I., Yamazaki K., Kimoto T., Minekawa Y., Utagawa E., Yamazaki S., Inouye S., Grohmann G. S., Monroe S. S., Stine S. E. A large outbreak of acute gastroenteritis associated with astrovirus among students and teachers in Osaka, Japan. J Infect Dis. 1994 Aug;170(2):439–443. doi: 10.1093/infdis/170.2.439. [DOI] [PubMed] [Google Scholar]
  32. Sanchez-Fauquier A., Carrascosa A. L., Carrascosa J. L., Otero A., Glass R. I., Lopez J. A., San Martin C., Melero J. A. Characterization of a human astrovirus serotype 2 structural protein (VP26) that contains an epitope involved in virus neutralization. Virology. 1994 Jun;201(2):312–320. doi: 10.1006/viro.1994.1296. [DOI] [PubMed] [Google Scholar]
  33. Willcocks M. M., Ashton N., Kurtz J. B., Cubitt W. D., Carter M. J. Cell culture adaptation of astrovirus involves a deletion. J Virol. 1994 Sep;68(9):6057–6058. doi: 10.1128/jvi.68.9.6057-6058.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Willcocks M. M., Brown T. D., Madeley C. R., Carter M. J. The complete sequence of a human astrovirus. J Gen Virol. 1994 Jul;75(Pt 7):1785–1788. doi: 10.1099/0022-1317-75-7-1785. [DOI] [PubMed] [Google Scholar]
  35. Willcocks M. M., Carter M. J. Identification and sequence determination of the capsid protein gene of human astrovirus serotype 1. FEMS Microbiol Lett. 1993 Nov 15;114(1):1–7. doi: 10.1111/j.1574-6968.1993.tb06542.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Willcocks M. M., Carter M. J., Laidler F. R., Madeley C. R. Growth and characterisation of human faecal astrovirus in a continuous cell line. Arch Virol. 1990;113(1-2):73–81. doi: 10.1007/BF01318354. [DOI] [PubMed] [Google Scholar]
  37. Xu A. Y., Wan X. B., Qiu F. X., Pang Q. F. Astrovirus in autumn infantile gastroenteritis. Chin Med J (Engl) 1981 Oct;94(10):659–662. [PubMed] [Google Scholar]

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