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. 1995 Apr;33(4):937–939. doi: 10.1128/jcm.33.4.937-939.1995

Parasitic culture of buffy coat for diagnosis of visceral leishmaniasis in human immunodeficiency virus-infected patients.

R López-Vélez 1, F Laguna 1, J Alvar 1, J A Pérez-Molina 1, R Molina 1, P Martinez 1, J Villarrubia 1
PMCID: PMC228071  PMID: 7790464

Abstract

Two samples of buffy coat from the peripheral blood of 25 human immunodeficiency virus-positive patients with proven visceral leishmaniasis, as determined with a bone marrow aspirate (stain and culture), were cultured onto Schneider's and Novy-McNeal-Nicolle media. Hemoculture positivity was 67%. The average growing time was 10 days. This is an easy, noninvasive, and sensitive technique.

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Selected References

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