Abstract
The rapid and accurate identification of the Lancefield group of beta-hemolytic streptococci and enterococci is an important procedure in clinical laboratories. Latex agglutination techniques are more rapid and technically less demanding than traditional extraction-precipitation methods. Prolex (Pro-Lab Diagnostics, Richmond Hill, Ontario, Canada) is a latex agglutination kit which contains modified nitrous acid reagents to extract antigens and can be used to detect group D antigen in streptococci and enterococci, as well as group A, B, C, F, and G antigens. A total of 302 strains of streptococci and enterococci were tested with this kit. All streptococci of groups A (41 strains), B (39 strains), C (35 strains), D (3 strains), F (10 strains), and G (48 strains) were correctly grouped, as were 125 (97%) of 129 strains of enterococci. Prolex is a reliable method for grouping the beta-hemolytic streptococci and enterococci most frequently encountered in clinical laboratories.
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Selected References
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- Birch B. R., Keaney M. G., Ganguli L. A. Antibiotic susceptibility and biochemical properties of Streptococcus faecalis strains reacting with both D and G antisera. J Clin Pathol. 1984 Nov;37(11):1289–1292. doi: 10.1136/jcp.37.11.1289. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Daly J. A., Seskin K. C. Evaluation of rapid, commercial latex techniques for serogrouping beta-hemolytic streptococci. J Clin Microbiol. 1988 Nov;26(11):2429–2431. doi: 10.1128/jcm.26.11.2429-2431.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Devriese L. A., Pot B., Collins M. D. Phenotypic identification of the genus Enterococcus and differentiation of phylogenetically distinct enterococcal species and species groups. J Appl Bacteriol. 1993 Nov;75(5):399–408. doi: 10.1111/j.1365-2672.1993.tb02794.x. [DOI] [PubMed] [Google Scholar]
- Freney J., Bland S., Etienne J., Desmonceaux M., Boeufgras J. M., Fleurette J. Description and evaluation of the semiautomated 4-hour rapid ID 32 Strep method for identification of streptococci and members of related genera. J Clin Microbiol. 1992 Oct;30(10):2657–2661. doi: 10.1128/jcm.30.10.2657-2661.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Petts D. N., Lane A., Kennedy P., Hadfield S. G., McIllmurray M. B. Direct detection of groups A, C and G streptococci in clinical specimens by a trivalent colour test. Eur J Clin Microbiol Infect Dis. 1988 Feb;7(1):34–39. doi: 10.1007/BF01962168. [DOI] [PubMed] [Google Scholar]
- You M. S., Facklam R. R. New test system for identification of Aerococcus, Enterococcus, and Streptococcus species. J Clin Microbiol. 1986 Oct;24(4):607–611. doi: 10.1128/jcm.24.4.607-611.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]