Abstract
In order to increase the database for in vitro growth and/or susceptibility testing in liquid media, we evaluated the growth of Helicobacter pylori in broth media containing 5% sheep blood. We also compared the effect of bismuth on the growth of H. pylori in broth media containing 10% fetal calf serum with the effect on growth in media containing 0.5% starch. In contrast to the result seen with agar, we found that sheep blood, whether whole or laked, inhibited the growth of H. pylori in broth media. In addition, we found that bismuth inhibited growth in media with starch but that this inhibition was negated in media with serum.
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- Axon A. T. Helicobacter pylori infection. J Antimicrob Chemother. 1993 Jul;32 (Suppl A):61–68. doi: 10.1093/jac/32.suppl_a.61. [DOI] [PubMed] [Google Scholar]
- Blaser M. J. Helicobacter pylori: its role in disease. Clin Infect Dis. 1992 Sep;15(3):386–391. doi: 10.1093/clind/15.3.386. [DOI] [PubMed] [Google Scholar]
- Buck G. E., Smith J. S. Medium supplementation for growth of Campylobacter pyloridis. J Clin Microbiol. 1987 Apr;25(4):597–599. doi: 10.1128/jcm.25.4.597-599.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coudron P. E., Kirby D. F. Comparison of rapid urease tests, staining techniques, and growth on different solid media for detection of Campylobacter pylori. J Clin Microbiol. 1989 Jul;27(7):1527–1530. doi: 10.1128/jcm.27.7.1527-1530.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coudron P. E., Stratton C. W. Utilization of time-kill kinetic methodologies for assessing the bactericidal activities of ampicillin and bismuth, alone and in combination, against Helicobacter pylori in stationary and logarithmic growth phases. Antimicrob Agents Chemother. 1995 Jan;39(1):66–69. doi: 10.1128/aac.39.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goodwin C. S., Armstrong J. A., Marshall B. J. Campylobacter pyloridis, gastritis, and peptic ulceration. J Clin Pathol. 1986 Apr;39(4):353–365. doi: 10.1136/jcp.39.4.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Graham D. Y. Campylobacter pylori and peptic ulcer disease. Gastroenterology. 1989 Feb;96(2 Pt 2 Suppl):615–625. doi: 10.1016/s0016-5085(89)80057-5. [DOI] [PubMed] [Google Scholar]
- Megraud F., Bonnet F., Garnier M., Lamouliatte H. Characterization of "Campylobacter pyloridis" by culture, enzymatic profile, and protein content. J Clin Microbiol. 1985 Dec;22(6):1007–1010. doi: 10.1128/jcm.22.6.1007-1010.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morgan D. R., Freedman R., Depew C. E., Kraft W. G. Growth of Campylobacter pylori in liquid media. J Clin Microbiol. 1987 Nov;25(11):2123–2125. doi: 10.1128/jcm.25.11.2123-2125.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]