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. 1995 May;33(5):1060–1063. doi: 10.1128/jcm.33.5.1060-1063.1995

Rapid and economical method for species identification of clinically significant coagulase-negative staphylococci.

M Ieven 1, J Verhoeven 1, S R Pattyn 1, H Goossens 1
PMCID: PMC228104  PMID: 7615705

Abstract

Four methods for the species identification of coagulase-negative staphylococci in the medical microbiology laboratory were compared with 444 consecutive isolates. The methods included (i) the reference method based on growth tests, (ii) API ID 32 Staph (bioMérieux), (iii) Staph-Zym (Rosco), and (iv) a rapid 4-h method developed in our laboratory (UZA method). The last method is based on the detection within 4 h of enzymatic activity of heavy bacterial suspensions in three substrate solutions (nongrowth tests). For 16.5% of the isolates some supplementary growth tests read after 24 h had to be added to the enzyme data for satisfactory identification. The reference method failed to identify four isolates. Of the 440 isolates identified by the reference method, API ID 32 Staph, Staph-Zym, and the UZA method correctly identified 419 (95.2%), 429 (97.5%), and 430 (97.7%) and misidentified 8 (1.8%), 4 (0.9%), and 1 (0.2%), respectively. Staphylococcus epidermidis, S. haemolyticus, S. lugdunensis, S. schleiferi, and S. capitis were identified with an accuracy of 98 to 100% by all the systems tested. S. capitis subsp. ureolyticus was not recognized by the API ID 32 system because the biochemical profiles for it are not yet included in the corresponding database. Whereas API ID 32 identified all 13 S. warneri isolates, both Staph-Zym and the UZA method missed 2 of these. S. hominis was identified with the least accuracy by the API ID 32 system (26 of 39 isolates), whereas the UZA and Staph-Zym methods identified 36 of the isolates belonging to this species.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Bannerman T. L., Kleeman K. T., Kloos W. E. Evaluation of the Vitek Systems Gram-Positive Identification card for species identification of coagulase-negative staphylococci. J Clin Microbiol. 1993 May;31(5):1322–1325. doi: 10.1128/jcm.31.5.1322-1325.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Herchline T. E., Ayers L. W. Occurrence of Staphylococcus lugdunensis in consecutive clinical cultures and relationship of isolation to infection. J Clin Microbiol. 1991 Mar;29(3):419–421. doi: 10.1128/jcm.29.3.419-421.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kamath U., Singer C., Isenberg H. D. Clinical significance of Staphylococcus warneri bacteremia. J Clin Microbiol. 1992 Feb;30(2):261–264. doi: 10.1128/jcm.30.2.261-264.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kleeman K. T., Bannerman T. L., Kloos W. E. Species distribution of coagulase-negative staphylococcal isolates at a community hospital and implications for selection of staphylococcal identification procedures. J Clin Microbiol. 1993 May;31(5):1318–1321. doi: 10.1128/jcm.31.5.1318-1321.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kloos W. E., Bannerman T. L. Update on clinical significance of coagulase-negative staphylococci. Clin Microbiol Rev. 1994 Jan;7(1):117–140. doi: 10.1128/cmr.7.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Latorre M., Rojo P. M., Unzaga M. J., Cisterna R. Staphylococcus schleiferi: a new opportunistic pathogen. Clin Infect Dis. 1993 Apr;16(4):589–590. doi: 10.1093/clind/16.4.589. [DOI] [PubMed] [Google Scholar]
  7. Mainardi J. L., Lortholary O., Buu-Hoï A., Desplaces N., Goldstein F., Gutmann L., Acar J. F. Native valve endocarditis caused by Staphylococcus capitis. Eur J Clin Microbiol Infect Dis. 1993 Oct;12(10):789–791. doi: 10.1007/BF02098474. [DOI] [PubMed] [Google Scholar]
  8. Pattyn S. R., Ieven M., Buffet L. Comparative evaluation of the Rapid ID 32A kit system, miniaturized standard procedure and a rapid fermentation procedure for the identification of anaerobic bacteria. Acta Clin Belg. 1993;48(2):81–85. doi: 10.1080/17843286.1993.11718291. [DOI] [PubMed] [Google Scholar]
  9. Pattyn S. R., Sion J. P., Verhoeven J. Evaluation of the LOGIC system for the rapid identification of members of the family Enterobacteriaceae in the clinical microbiology laboratory. J Clin Microbiol. 1990 Jun;28(6):1449–1450. doi: 10.1128/jcm.28.6.1449-1450.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Perry J. D., Ford M., Hjersing N., Gould F. K. Rapid conventional scheme for biochemical identification of antibiotic resistant enterobacteriaceae isolates from urine. J Clin Pathol. 1988 Sep;41(9):1010–1012. doi: 10.1136/jcp.41.9.1010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pfaller M. A., Herwaldt L. A. Laboratory, clinical, and epidemiological aspects of coagulase-negative staphylococci. Clin Microbiol Rev. 1988 Jul;1(3):281–299. doi: 10.1128/cmr.1.3.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Refsahl K., Andersen B. M. Clinically significant coagulase-negative staphylococci: identification and resistance patterns. J Hosp Infect. 1992 Sep;22(1):19–31. doi: 10.1016/0195-6701(92)90127-8. [DOI] [PubMed] [Google Scholar]
  13. Ringberg H., Thorén A., Bredberg A. Evaluation of coagulase-negative staphylococci in blood cultures. A prospective clinical and microbiological study. Scand J Infect Dis. 1991;23(3):315–323. doi: 10.3109/00365549109024317. [DOI] [PubMed] [Google Scholar]
  14. Rupp M. E., Archer G. L. Coagulase-negative staphylococci: pathogens associated with medical progress. Clin Infect Dis. 1994 Aug;19(2):231–245. doi: 10.1093/clinids/19.2.231. [DOI] [PubMed] [Google Scholar]
  15. Shuttleworth R., Colby W. D. Staphylococcus lugdunensis endocarditis. J Clin Microbiol. 1992 Aug;30(8):1948–1952. doi: 10.1128/jcm.30.8.1948-1952.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stager C. E., Davis J. R. Automated systems for identification of microorganisms. Clin Microbiol Rev. 1992 Jul;5(3):302–327. doi: 10.1128/cmr.5.3.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Surani S., Chandna H., Weinstein R. A. Breast abscess: coagulase-negative staphylococcus as a sole pathogen. Clin Infect Dis. 1993 Oct;17(4):701–704. doi: 10.1093/clinids/17.4.701. [DOI] [PubMed] [Google Scholar]
  18. Vandenesch F., Etienne J., Reverdy M. E., Eykyn S. J. Endocarditis due to Staphylococcus lugdunensis: report of 11 cases and review. Clin Infect Dis. 1993 Nov;17(5):871–876. doi: 10.1093/clinids/17.5.871. [DOI] [PubMed] [Google Scholar]
  19. van Dyck E., Piot P., Pattyn S. R. Evaluation of three techniques for carbohydrate fermentation of Neisseriae. Zentralbl Bakteriol Orig A. 1976 Nov;236(2-3):231–234. [PubMed] [Google Scholar]

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