Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1995 May;33(5):1084–1088. doi: 10.1128/jcm.33.5.1084-1088.1995

Comparison of automated Difco ESP blood culture system with biphasic BBL Septi-Chek system for detection of bloodstream infections in pediatric patients.

P L Welby 1, D S Keller 1, G A Storch 1
PMCID: PMC228109  PMID: 7615710

Abstract

We compared the Difco ESP 384 blood culture system with the pediatric Septi-Chek system for the detection of bloodstream infections in pediatric patients. A total of 10,762 blood culture sets included an ESP aerobic bottle and a Septi-Chek bottle. From these cultures, a total of 278 organisms classified as probable pathogens were isolated, including 237 from ESP bottles and 221 from Septi-Chek bottles. This difference was not statistically significant. More organisms classified as possible contaminants were also isolated from ESP bottles (for ESP, 480 bottles; for Septi-Chek, 418 bottles; P < 0.01). The time to detection was shorter for probable pathogens isolated from ESP bottles (median times for organisms isolated from both systems: ESP, 14.0 h; Septi-Chek, 34.5 h; P < 0.001). The proportions of all probable pathogens detected by 24 and 48 h after inoculation were 78 and 96%, respectively, for ESP compared with 31 and 74%, respectively, for Septi-Chek. The time to final identification was also shorter for organisms grown in ESP bottles (median times for organisms isolated from both systems: ESP, 48.8 h; Septi-Chek, 58.5 h; P < or = 0.001). A subset of 4,442 cultures also included an ESP anaerobic bottle in addition to an ESP aerobic bottle and a Septi-Chek bottle. There were no significant differences in the recovery of probable pathogens by any of the possible two bottle combinations, but five anaerobic pathogens were recovered only in the anaerobic bottle.(ABSTRACT TRUNCATED AT 250 WORDS)

Full Text

The Full Text of this article is available as a PDF (173.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crosson F. J., Jr, Feder H. M., Jr, Bocchini J. A., Jr, Hackell J. M., Hackell J. G. Neonatal sepsis at The Johns Hopkins Hospital, 1969-1975: bacterial isolates and clinical correlates. Johns Hopkins Med J. 1977 Feb;140(2):37–46. [PubMed] [Google Scholar]
  2. Dunkle L. M., Brotherton T. J., Feigin R. D. Anaerobic infections in children: a prospective study. Pediatrics. 1976 Mar;57(3):311–320. [PubMed] [Google Scholar]
  3. Echeverria P., Smith A. L. Anaerobic bacteremia as observed in a children's hospital. Clinically this may signify true anaerobic sepsis. Clin Pediatr (Phila) 1978 Sep;17(9):688–690. doi: 10.1177/000992287801700904. [DOI] [PubMed] [Google Scholar]
  4. Harrod J. R., Stevens D. A. Anaerobic infections in the newborn infant. J Pediatr. 1974 Sep;85(3):399–402. doi: 10.1016/s0022-3476(74)80129-0. [DOI] [PubMed] [Google Scholar]
  5. Morello J. A., Leitch C., Nitz S., Dyke J. W., Andruszewski M., Maier G., Landau W., Beard M. A. Detection of bacteremia by Difco ESP blood culture system. J Clin Microbiol. 1994 Mar;32(3):811–818. doi: 10.1128/jcm.32.3.811-818.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Neal P. R., Kleiman M. B., Reynolds J. K., Allen S. D., Lemons J. A., Yu P. L. Volume of blood submitted for culture from neonates. J Clin Microbiol. 1986 Sep;24(3):353–356. doi: 10.1128/jcm.24.3.353-356.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Noel G. J., Laufer D. A., Edelson P. J. Anaerobic bacteremia in a neonatal intensive care unit: an eighteen-year experience. Pediatr Infect Dis J. 1988 Dec;7(12):858–862. [PubMed] [Google Scholar]
  8. Nolte F. S., Williams J. M., Jerris R. C., Morello J. A., Leitch C. D., Matushek S., Schwabe L. D., Dorigan F., Kocka F. E. Multicenter clinical evaluation of a continuous monitoring blood culture system using fluorescent-sensor technology (BACTEC 9240). J Clin Microbiol. 1993 Mar;31(3):552–557. doi: 10.1128/jcm.31.3.552-557.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. St Geme J. W., 3rd, Bell L. M., Baumgart S., D'Angio C. T., Harris M. C. Distinguishing sepsis from blood culture contamination in young infants with blood cultures growing coagulase-negative staphylococci. Pediatrics. 1990 Aug;86(2):157–162. [PubMed] [Google Scholar]
  10. Thorpe T. C., Wilson M. L., Turner J. E., DiGuiseppi J. L., Willert M., Mirrett S., Reller L. B. BacT/Alert: an automated colorimetric microbial detection system. J Clin Microbiol. 1990 Jul;28(7):1608–1612. doi: 10.1128/jcm.28.7.1608-1612.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Trenholme G. M., Kaplan R. L., Karakusis P. H., Stine T., Fuhrer J., Landau W., Levin S. Clinical impact of rapid identification and susceptibility testing of bacterial blood culture isolates. J Clin Microbiol. 1989 Jun;27(6):1342–1345. doi: 10.1128/jcm.27.6.1342-1345.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Washington J. A. Blood cultures: an overview. Eur J Clin Microbiol Infect Dis. 1989 Sep;8(9):803–806. doi: 10.1007/BF02185852. [DOI] [PubMed] [Google Scholar]
  13. Welby P. L., Zusag T. M., Orlando M. F., Storch G. A. Evaluation of the pediatric Septi-Chek blood culture system. Diagn Microbiol Infect Dis. 1992 May-Jun;15(4):301–306. doi: 10.1016/0732-8893(92)90015-l. [DOI] [PubMed] [Google Scholar]
  14. Welby P. L., Zusag T. M., Storch G. A. Comparison of the Bactec Peds Plus pediatric blood culture vial with Roche pediatric Septi-Chek for blood cultures from pediatric patients. J Clin Microbiol. 1992 May;30(5):1361–1362. doi: 10.1128/jcm.30.5.1361-1362.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES