Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1995 May;33(5):1136–1140. doi: 10.1128/jcm.33.5.1136-1140.1995

Differentiation of Campylobacter jejuni and Campylobacter coli strains by using restriction endonuclease DNA profiles and DNA fragment polymorphisms.

V Korolik 1, L Moorthy 1, P J Coloe 1
PMCID: PMC228118  PMID: 7615717

Abstract

The chromosomal DNA fragment patterns from a total of 169 Campylobacter jejuni and Campylobacter coli isolates from poultry and humans were analyzed by using DNA restriction endonucleases ClaI and EcoRV. The DNA restriction patterns produced by ClaI and EcoRV consisted of unique DNA fragments of 9 to 9.5 kb and 3.5 kb generated with ClaI and a single unique fragment of 3.0 kb produced by EcoRV. These patterns were obtained with all strains of C. jejuni tested. The DNA restriction patterns were further examined by Southern blot analysis with a previously constructed DNA probe, pMO2005, which is also able to distinguish between C. jejuni and C. coli spp. (5). Two types of patterns were produced by hybridization with the ClaI-cleaved DNA of C. jejuni strains, one of a single 18.5-kb genomic fragment and the other of 14.5- and 4.0-kb fragments. This indicated the presence of an extra ClaI site in this genomic fragment in the strains with the duplex pattern. The Southern blot analysis of 169 C. jejuni and C. coli isolates from poultry and from humans with DNA probe pMO2005 demonstrated that 78% of C. jejuni strains isolated from chickens hybridized with DNA probe pMO2005 with a characteristic 14.5- and 4.0-kb banding pattern and 22% hybridized with a single 18.5-kb fragment, whereas 71% of human isolates hybridized with the single 18.5-kb fragment and only 29% hybridized with 14.5- and 4.0-kb fragments. These findings suggest that only a small proportion of C. jejuni strains that colonize chickens may cause disease in humans.

Full Text

The Full Text of this article is available as a PDF (538.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aeschbacher M., Piffaretti J. C. Population genetics of human and animal enteric Campylobacter strains. Infect Immun. 1989 May;57(5):1432–1437. doi: 10.1128/iai.57.5.1432-1437.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Albert M. J., Leach A., Asche V., Hennessy J., Penner J. L. Serotype distribution of Campylobacter jejuni and Campylobacter coli isolated from hospitalized patients with diarrhea in central Australia. J Clin Microbiol. 1992 Jan;30(1):207–210. doi: 10.1128/jcm.30.1.207-210.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blaser M. J., Taylor D. N., Feldman R. A. Epidemiology of Campylobacter jejuni infections. Epidemiol Rev. 1983;5:157–176. doi: 10.1093/oxfordjournals.epirev.a036256. [DOI] [PubMed] [Google Scholar]
  4. Fraser A. D., Brooks B. W., Garcia M. M., Lior H. Molecular discrimination of Campylobacter coli serogroup 20 biotype I (Lior) strains. Vet Microbiol. 1992 Feb;30(2-3):267–280. doi: 10.1016/0378-1135(92)90120-i. [DOI] [PubMed] [Google Scholar]
  5. Korolik V., Coloe P. J., Krishnapillai V. A specific DNA probe for the identification of Campylobacter jejuni. J Gen Microbiol. 1988 Feb;134(2):521–529. doi: 10.1099/00221287-134-2-521. [DOI] [PubMed] [Google Scholar]
  6. Lin G. F., Gebhart C. J., Murtaugh M. P. Southern blot analysis of strain variation in Campylobacter mucosalis. Vet Microbiol. 1991 Feb 1;26(3):279–289. doi: 10.1016/0378-1135(91)90021-7. [DOI] [PubMed] [Google Scholar]
  7. Owen R. J., Hernandez J. Genotypic variation in 'Campylobacter upsaliensis' from blood and faeces of patients in different countries. FEMS Microbiol Lett. 1990 Oct;60(1-2):5–10. doi: 10.1016/0378-1097(90)90335-n. [DOI] [PubMed] [Google Scholar]
  8. Patton C. M., Wachsmuth I. K., Evins G. M., Kiehlbauch J. A., Plikaytis B. D., Troup N., Tompkins L., Lior H. Evaluation of 10 methods to distinguish epidemic-associated Campylobacter strains. J Clin Microbiol. 1991 Apr;29(4):680–688. doi: 10.1128/jcm.29.4.680-688.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wesley I. V., Bryner J. H. Antigenic and restriction enzyme analysis of isolates of Campylobacter fetus subsp venerealis recovered from persistently infected cattle. Am J Vet Res. 1989 Jun;50(6):807–813. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES