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. 1995 May;33(5):1158–1163. doi: 10.1128/jcm.33.5.1158-1163.1995

Bovine vaginal antibody responses to immunoaffinity-purified surface antigen of Tritrichomonas foetus.

J S Ikeda 1, R H BonDurant 1, L B Corbeil 1
PMCID: PMC228123  PMID: 7615722

Abstract

Bovine trichomoniasis is a prevalent sexually transmitted disease of cattle caused by the protozoan Tritrichomonas foetus. Currently, diagnosis is most often made by culture. In order to provide a faster immunodiagnostic approach, a specific enzyme-linked immunosorbent assay (ELISA) was investigated. A protective surface antigen (TF1.17 antigen) of T. foetus was immunoaffinity purified and used in an ELISA to detect antibodies in vaginal mucus from heifers inoculated with T. foetus. In preliminary studies, antibodies of the immunoglobulin A (IgA) isotype were detected in mucus from all experimentally infected heifers which were tested at 6 weeks postinoculation, whereas IgG1 and IgG2 were not. In addition, IgA responses detected in postinoculation samples were all greater than those detected in preinoculation samples, unlike those detected by a whole-cell antigen ELISA. For these two reasons, IgA antibodies appeared to be useful diagnostically. Further investigation of IgA antibodies used vaginal mucus collected weekly from heifers inoculated intravaginally with 10(2), 10(4), or 10(6) T. foetus organisms. Heifers with positive cultures for T. foetus had similar IgA responses to TF1.17 antigen over the 10 weeks of infection regardless of the initial inoculum dose. This indicates that if the dose is sufficient to establish infection, the magnitude and duration of the immune response are no longer dependent on dose. All of the infected animals receiving all dosages responded with high absorbance values in the IgA anti-TF1.17 antigen ELISA by 6 weeks postinoculation, and all absorbance values remained high at 10 weeks. To determine the duration of the IgA response, four other heifers inoculated with 7 x 10(6) T. foetus organisms were studied through 24 weeks postinoculation.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. BonDurant R. H., Anderson M. L., Blanchard P., Hird D., Danaye-Elmi C., Palmer C., Sischo W. M., Suther D., Utterback W., Weigler B. J. Prevalence of trichomoniasis among California beef herds. J Am Vet Med Assoc. 1990 May 15;196(10):1590–1593. [PubMed] [Google Scholar]
  2. BonDurant R. H., Corbeil R. R., Corbeil L. B. Immunization of virgin cows with surface antigen TF1.17 of Tritrichomonas foetus. Infect Immun. 1993 Apr;61(4):1385–1394. doi: 10.1128/iai.61.4.1385-1394.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burgess D. E. Clonal and geographic distribution of a surface antigen of Tritrichomonas foetus. J Protozool. 1988 Feb;35(1):119–122. doi: 10.1111/j.1550-7408.1988.tb04090.x. [DOI] [PubMed] [Google Scholar]
  4. Campero C. M., Ballabene N. C., Cipolla A. C., Zamora A. S. Dual infection of bulls with campylobacteriosis and trichomoniasis: treatment with dimetridazole chlorhydrate. Aust Vet J. 1987 Oct;64(10):320–321. doi: 10.1111/j.1751-0813.1987.tb07344.x. [DOI] [PubMed] [Google Scholar]
  5. Goodger W. J., Skirrow S. Z. Epidemiologic and economic analyses of an unusually long epizootic of trichomoniasis in a large California dairy herd. J Am Vet Med Assoc. 1986 Oct 1;189(7):772–776. [PubMed] [Google Scholar]
  6. Gorton K. A., Fulton R. W., Fox J. C., Rice L. E. Fluorometric measurement of IgG antibody responses in cattle vaccinated with Tritrichomonas foetus. Can J Vet Res. 1993 Apr;57(2):114–118. [PMC free article] [PubMed] [Google Scholar]
  7. Hall M. R., Huang J. C., Ota R., Redelman D., Hanks D., Taylor R. E. Characterization of Tritrichomonas foetus antigens, using bovine antiserum. Am J Vet Res. 1986 Dec;47(12):2549–2553. [PubMed] [Google Scholar]
  8. Ho M. S., Conrad P. A., Conrad P. J., LeFebvre R. B., Perez E., BonDurant R. H. Detection of bovine trichomoniasis with a specific DNA probe and PCR amplification system. J Clin Microbiol. 1994 Jan;32(1):98–104. doi: 10.1128/jcm.32.1.98-104.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hodgson J. L., Jones D. W., Widders P. R., Corbeil L. B. Characterization of Tritrichomonas foetus antigens by use of monoclonal antibodies. Infect Immun. 1990 Sep;58(9):3078–3083. doi: 10.1128/iai.58.9.3078-3083.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Huang J. C., Hanks D., Kvasnicka W., Hanks M., Hall M. R. Antigenic relationship among field isolates of Tritrichomonas foetus from cattle. Am J Vet Res. 1989 Jul;50(7):1064–1068. [PubMed] [Google Scholar]
  11. Ikeda J. S., BonDurant R. H., Campero C. M., Corbeil L. B. Conservation of a protective surface antigen of Tritrichomonas foetus. J Clin Microbiol. 1993 Dec;31(12):3289–3295. doi: 10.1128/jcm.31.12.3289-3295.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kvasnicka W. G., Taylor R. E., Huang J. C., Hanks D., Tronstad R. J., Bosomworth A., Hall M. R. Investigations of the incidence of bovine trichomoniasis in nevada and of the efficacy of immunizing cattle with vaccines containing Tritrichomonas foetus. Theriogenology. 1989 May;31(5):963–971. doi: 10.1016/0093-691x(89)90479-2. [DOI] [PubMed] [Google Scholar]
  13. Parsonson I. M., Clark B. L., Dufty J. H. Early pathogenesis and pathology of Tritrichomonas foetus infection in virgin heifers. J Comp Pathol. 1976 Jan;86(1):59–66. doi: 10.1016/0021-9975(76)90028-1. [DOI] [PubMed] [Google Scholar]
  14. Reece R. L., Dennett D. P., Johnson R. H. Tritrichomonas foetus agglutination tests upon samples collected from cattle: cross-reactions associated with vaccination against Campylobacter fetus subsp venerealis. Aust Vet J. 1981 Jul;57(7):352–353. doi: 10.1111/j.1751-0813.1981.tb05853.x. [DOI] [PubMed] [Google Scholar]
  15. Skirrow S. Z., BonDurant R. H. Immunoglobulin isotype of specific antibodies in reproductive tract secretions and sera in Tritrichomonas foetus-infected heifers. Am J Vet Res. 1990 Apr;51(4):645–653. [PubMed] [Google Scholar]
  16. Skirrow S. Z., BonDurant R. H. Induced Tritrichomonas foetus infection in beef heifers. J Am Vet Med Assoc. 1990 Mar 15;196(6):885–889. [PubMed] [Google Scholar]
  17. Skirrow S., BonDurant R., Farley J., Correa J. Efficacy of ipronidazole against trichomoniasis in beef bulls. J Am Vet Med Assoc. 1985 Aug 15;187(4):405–407. [PubMed] [Google Scholar]
  18. Soto P., Parma A. E. The immune response in cattle infected with Tritrichomonas foetus. Vet Parasitol. 1989 Oct;33(3-4):343–348. doi: 10.1016/0304-4017(89)90143-x. [DOI] [PubMed] [Google Scholar]
  19. Yule A., Skirrow S. Z., Staats J., Bondurant R. H. Development and preliminary assessment of a polyclonal antibody-based enzyme immunoassay for the detection of Tritrichomonas foetus antigen in breeding cattle. Vet Parasitol. 1989 May;31(2):115–123. doi: 10.1016/0304-4017(89)90026-5. [DOI] [PubMed] [Google Scholar]

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