Abstract
The complement fixation (CF) test is the current reference serologic test for the diagnosis of Mycoplasma pneumoniae infection. However, it is reported to be insensitive and nonspecific, and it is labor intensive. To determine if a faster and more sensitive diagnosis of M. pneumoniae could be obtained, we examined 50 paired serum samples from patients with suspected M. pneumoniae infection by the CF test and two commercial rapid antibody detection kits, the Remel M. pneumoniae immunoglobulin G (IgG)-IgM antibody test system (Remel, Lenexa, Kans.) and the Seradyn Color Vue M. pneumoniae IgG-IgM kit (Seradyn, Indianapolis, Ind.). The Remel test, a 5-min qualitative immunobinding assay, detected antibodies in three patient serum samples with CF titers of 32 and in all but one sample with titers of > or = 64. The Seradyn test, a 40-min qualitative agglutination test, was less sensitive than CF or Remel. The Seradyn test was positive in 68% of cases, compared with 94 and 96% of cases tested by CF or Remel, respectively. Both commercial tests are faster and less technically demanding to perform than is the CF test.
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Selected References
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- Barker C. E., Sillis M., Wreghitt T. G. Evaluation of Serodia Myco II particle agglutination test for detecting Mycoplasma pneumoniae antibody: comparison with mu-capture ELISA and indirect immunofluorescence. J Clin Pathol. 1990 Feb;43(2):163–165. doi: 10.1136/jcp.43.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bernet C., Garret M., de Barbeyrac B., Bebear C., Bonnet J. Detection of Mycoplasma pneumoniae by using the polymerase chain reaction. J Clin Microbiol. 1989 Nov;27(11):2492–2496. doi: 10.1128/jcm.27.11.2492-2496.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Busolo F., Tonin E., Meloni G. A. Enzyme-linked immunosorbent assay for serodiagnosis of Mycoplasma pneumoniae infections. J Clin Microbiol. 1983 Aug;18(2):432–435. doi: 10.1128/jcm.18.2.432-435.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DOWDLE W. R., ROBINSON R. Q. AN INDIRECT HEMAGGLUTINATION TEST FOR DIAGNOSIS OF MYCOPLASMA PNEUMONIAE INFECTIONS. Proc Soc Exp Biol Med. 1964 Aug-Sep;116:947–950. doi: 10.3181/00379727-116-29416. [DOI] [PubMed] [Google Scholar]
- Jacobs E. Serological diagnosis of Mycoplasma pneumoniae infections: a critical review of current procedures. Clin Infect Dis. 1993 Aug;17 (Suppl 1):S79–S82. doi: 10.1093/clinids/17.supplement_1.s79. [DOI] [PubMed] [Google Scholar]
- Jensen J. S., Søndergård-Andersen J., Uldum S. A., Lind K. Detection of Mycoplasma pneumoniae in simulated clinical samples by polymerase chain reaction. Brief report. APMIS. 1989 Nov;97(11):1046–1048. doi: 10.1111/j.1699-0463.1989.tb00516.x. [DOI] [PubMed] [Google Scholar]
- Karppelin M., Hakkarainen K., Kleemola M., Miettinen A. Comparison of three serological methods for diagnosing Mycoplasma pneumoniae infection. J Clin Pathol. 1993 Dec;46(12):1120–1123. doi: 10.1136/jcp.46.12.1120. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kenny G. E., Kaiser G. G., Cooney M. K., Foy H. M. Diagnosis of Mycoplasma pneumoniae pneumonia: sensitivities and specificities of serology with lipid antigen and isolation of the organism on soy peptone medium for identification of infections. J Clin Microbiol. 1990 Sep;28(9):2087–2093. doi: 10.1128/jcm.28.9.2087-2093.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kok T. W., Varkanis G., Marmion B. P., Martin J., Esterman A. Laboratory diagnosis of Mycoplasma pneumoniae infection. 1. Direct detection of antigen in respiratory exudates by enzyme immunoassay. Epidemiol Infect. 1988 Dec;101(3):669–684. doi: 10.1017/s0950268800029551. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leland D. S., Barth K. A., Cunningham E. B. Comparison of the Seradyn Color Vue passive agglutination test and complement fixation for detection of Mycoplasma pneumoniae antibodies. J Clin Microbiol. 1993 Apr;31(4):1013–1015. doi: 10.1128/jcm.31.4.1013-1015.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lüneberg E., Jensen J. S., Frosch M. Detection of Mycoplasma pneumoniae by polymerase chain reaction and nonradioactive hybridization in microtiter plates. J Clin Microbiol. 1993 May;31(5):1088–1094. doi: 10.1128/jcm.31.5.1088-1094.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marmion B. P., Williamson J., Worswick D. A., Kok T. W., Harris R. J. Experience with newer techniques for the laboratory detection of Mycoplasma pneumoniae infection: Adelaide, 1978-1992. Clin Infect Dis. 1993 Aug;17 (Suppl 1):S90–S99. doi: 10.1093/clinids/17.supplement_1.s90. [DOI] [PubMed] [Google Scholar]
- Shearman M. J., Cubie H. A., Inglis J. M. Mycoplasma pneumoniae infection: early diagnosis by detection of specific IgM by immunofluorescence. Br J Biomed Sci. 1993 Dec;50(4):305–308. [PubMed] [Google Scholar]
- Sillis M. The limitations of IgM assays in the serological diagnosis of Mycoplasma pneumoniae infections. J Med Microbiol. 1990 Dec;33(4):253–258. doi: 10.1099/00222615-33-4-253. [DOI] [PubMed] [Google Scholar]
- Tully J. G., Rose D. L., Whitcomb R. F., Wenzel R. P. Enhanced isolation of Mycoplasma pneumoniae from throat washings with a newly-modified culture medium. J Infect Dis. 1979 Apr;139(4):478–482. doi: 10.1093/infdis/139.4.478. [DOI] [PubMed] [Google Scholar]