Abstract
Direct enzyme immunoassay (Herpchek) was compared with culture on 21,522 specimens mainly from asymptomatic women for herpes simplex virus detection. Sensitivity and specificity were 73.8 and 97.7%, respectively. The 33% detection rate by enzyme immunoassay in 5 h increased to 43% when the enzyme immunoassay was combined with culture. Herpchek alone was not sensitive enough, but in combination with culture, maximum detection was achieved.
Full Text
The Full Text of this article is available as a PDF (135.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baker D. A., Pavan-Langston D., Gonik B., Milch P. O., Dunkel E. C., Berkowitz A., Woodin M. B., Philip A., Vander-Mallie R. J., Bobrow M. N. Multicenter clinical evaluation of the Du Pont Herpchek HSV ELISA, a new rapid diagnostic test for the direct detection of herpes simplex virus. Adv Exp Med Biol. 1990;263:71–76. doi: 10.1007/978-1-4613-0601-6_6. [DOI] [PubMed] [Google Scholar]
- Cone R. W., Swenson P. D., Hobson A. C., Remington M., Corey L. Herpes simplex virus detection from genital lesions: a comparative study using antigen detection (HerpChek) and culture. J Clin Microbiol. 1993 Jul;31(7):1774–1776. doi: 10.1128/jcm.31.7.1774-1776.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dascal A., Chan-Thim J., Morahan M., Portnoy J., Mendelson J. Diagnosis of herpes simplex virus infection in a clinical setting by a direct antigen detection enzyme immunoassay kit. J Clin Microbiol. 1989 Apr;27(4):700–704. doi: 10.1128/jcm.27.4.700-704.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dascal A., Chan-Thim J., Morahan M., Portnoy J., Mendelson J. Replacement of special enzyme immunoassay transport medium by a standard viral transport medium in the Herpcheck herpes simplex virus antigen detection test. Diagn Microbiol Infect Dis. 1989 Nov-Dec;12(6):473–475. doi: 10.1016/0732-8893(89)90080-1. [DOI] [PubMed] [Google Scholar]
- Dunkel E. C., Pavan-Langston D., Fitzpatrick K., Cukor G. Rapid detection of herpes simplex virus (HSV) antigen in human ocular infections. Curr Eye Res. 1988 Jul;7(7):661–666. doi: 10.3109/02713688809033194. [DOI] [PubMed] [Google Scholar]
- Gonik B., Seibel M., Berkowitz A., Woodin M. B., Mills K. Comparison of two enzyme-linked immunosorbent assays for detection of herpes simplex virus antigen. J Clin Microbiol. 1991 Mar;29(3):436–438. doi: 10.1128/jcm.29.3.436-438.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koutsky L. A., Stevens C. E., Holmes K. K., Ashley R. L., Kiviat N. B., Critchlow C. W., Corey L. Underdiagnosis of genital herpes by current clinical and viral-isolation procedures. N Engl J Med. 1992 Jun 4;326(23):1533–1539. doi: 10.1056/NEJM199206043262305. [DOI] [PubMed] [Google Scholar]
- Verano L., Michalski F. J. Herpes simplex virus antigen direct detection in standard virus transport medium by Du Pont Herpchek enzyme-linked immunosorbent assay. J Clin Microbiol. 1990 Nov;28(11):2555–2558. doi: 10.1128/jcm.28.11.2555-2558.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Verano L., Michalski F. J. Spin-amplified culture followed by enzyme immunoassay for detection of herpes simplex virus in patient specimens: a comparative study. Clin Diagn Virol. 1993 Mar;1(1):23–28. doi: 10.1016/0928-0197(93)90030-9. [DOI] [PubMed] [Google Scholar]
- Wu T. C., Zaza S., Callaway J. Evaluation of the Du Pont HERPCHEK herpes simplex virus antigen test with clinical specimens. J Clin Microbiol. 1989 Aug;27(8):1903–1905. doi: 10.1128/jcm.27.8.1903-1905.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]