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. 1995 May;33(5):1380–1382. doi: 10.1128/jcm.33.5.1380-1382.1995

Detection of antibodies against human papillomavirus (HPV) type 16 virions by enzyme-linked immunosorbent assay using recombinant HPV 16 L1 capsids produced by recombinant baculovirus.

P Le Cann 1, A Touze 1, N Enogat 1, D Leboulleux 1, C Mougin 1, M C Legrand 1, C Calvet 1, J M Afoutou 1, P Coursaget 1
PMCID: PMC228172  PMID: 7615761

Abstract

The L1 major capsid protein of human papillomavirus type 16 (HPV-16) was expressed in Sf-21 insect cells with a recombinant baculovirus. Virus-like particles obtained were purified and used to develop an enzyme-linked immunosorbent assay for detection of anti-HPV-16 antibodies in sera from 76 women with evidence of genital HPV infection and 79 controls. HPV-16-infected individuals developed antibodies directed at HPV-16 virions since reactivity against recombinant HPV-16L1 capsids was observed in 50% of them compared with only 6% in the general adult population. However, some cross-reactivities with sera from women infected with others HPV types were observed.

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Selected References

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