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. 1995 May;33(5):1411–1413. doi: 10.1128/jcm.33.5.1411-1413.1995

Cell culture-PCR technique for detection of infectious cytomegalovirus in peripheral blood.

S M Lipson 1, A B Ashraf 1, S H Lee 1, M H Kaplan 1, D H Shepp 1
PMCID: PMC228181  PMID: 7615769

Abstract

We determined the efficacy of PCR for the direct detection of infectious cytomegalovirus (CMV) in specimen-inoculated MRC-5 tube cultures (cell culture-PCR [CC-PCR]). Parallel testing was performed by a shell vial assay-indirect immunofluorescence assay (SVA-IFA) and isolation (conventional MRC tube cultures [TC-CPE]. The sensitivity, specificity, and positive and negative predictive values for CC-PCR, SVA-IFA, and TC-CPE were 81, 99, 94, and 94%, 86, 100, 100, and 96%, and 77, 100, 100, and 93%, respectively. Future application of CC-PCR may be directed toward its use as a screening tool for cytomegalovirus genotypic variants.

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Selected References

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