Abstract
In initial studies of slide and tube agglutination tests of shigella-like isolates from diarrheal stools from Bangladesh, 10 and 8 strains of Shigella dysenteriae were identified as provisional serovars (serovars that have been defined but not given a number in the antigenic schema) E22383 and E23507, respectively. Further screening of diarrheal stool isolates in a slide agglutination test with antisera (made with the international reference strains) to the two provisional serovars of S. dysenteriae identified an additional 36 isolates as belonging to E22383 and 8 isolates as belonging to E23507. All strains had properties typical of invasive Shigella strains in that they were positive in the Sereny test and had the 120- to 140-MDa plasmid associated with invasiveness. On the basis of these markers of pathogenicity and isolation from various geographical locations, now we recommend that provisional serovars E22383 and E23507 be designated S. dysenteriae serotypes 14 and 15, respectively.
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Selected References
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- Ansaruzzaman M., Kibriya A. K., Mitra A. K., Sack R. B., Albert M. J. Isolation of Shigella dysenteriae serotypes 11, 12, and 13 from patients with diarrhea in Bangladesh. J Clin Microbiol. 1993 May;31(5):1392–1393. doi: 10.1128/jcm.31.5.1392-1393.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
- Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gross R. J., Thomas L. V., Cheasty T., Rowe B., Lindberg A. A. Four new provisional serovars of Shigella. J Clin Microbiol. 1989 May;27(5):829–831. doi: 10.1128/jcm.27.5.829-831.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haider K., Malek M. A., Albert M. J. Occurrence of drug resistance in Shigella species isolated from patients with diarrhoea in Bangladesh. J Antimicrob Chemother. 1993 Sep;32(3):509–511. doi: 10.1093/jac/32.3.509. [DOI] [PubMed] [Google Scholar]
- SERENY B. Experimental keratoconjunctivitis shigellosa. Acta Microbiol Acad Sci Hung. 1957;4(4):367–376. [PubMed] [Google Scholar]
- Sansonetti P. J., Kopecko D. J., Formal S. B. Involvement of a plasmid in the invasive ability of Shigella flexneri. Infect Immun. 1982 Mar;35(3):852–860. doi: 10.1128/iai.35.3.852-860.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stoll B. J., Glass R. I., Huq M. I., Khan M. U., Banu H., Holt J. Epidemiologic and clinical features of patients infected with Shigella who attended a diarrheal disease hospital in Bangladesh. J Infect Dis. 1982 Aug;146(2):177–183. doi: 10.1093/infdis/146.2.177. [DOI] [PubMed] [Google Scholar]
- Wathen-Grady H. G., Britt L. E., Strockbine N. A., Wachsmuth I. K. Characterization of Shigella dysenteriae serotypes 11, 12, and 13. J Clin Microbiol. 1990 Nov;28(11):2580–2584. doi: 10.1128/jcm.28.11.2580-2584.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]