Abstract
A new selective medium (BAV), consisting of trypticase agar with 5% sheep hemoglobin and 2 micrograms of vancomycin per ml, was compared with the routine blood-agar medium for the primary isolation of Kingella kingae from upper respiratory specimens from a population of young children. Infection was detected by the BAV medium in 43 of 44 (98%) cultures positive for K. kingae, and detection of the organism was facilitated by inhibition of gram-positive flora. Infection was detected in only 10 of 44 (23%) positive cultures by the blood-agar medium, and plates were usually covered by abundant normal flora, making the recognition of K. kingae much more difficult. Challenge of the medium with different organisms of respiratory origin showed that the BAV medium was inhibitory for gram-positive cocci and Haemophilus influenzae but that it supported growth of eight K. kingae strains isolated from patients with invasive infections. The new medium appears to be a useful epidemiological tool for studying the respiratory carriage of K. kingae.
Full Text
The Full Text of this article is available as a PDF (162.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
- Claesson B., Falsen E., Kjellman B. Kingella kingae infections: a review and a presentation of data from 10 Swedish cases. Scand J Infect Dis. 1985;17(3):233–243. doi: 10.3109/inf.1985.17.issue-3.01. [DOI] [PubMed] [Google Scholar]
- Goutzmanis J. J., Gonis G., Gilbert G. L. Kingella kingae infection in children: ten cases and a review of the literature. Pediatr Infect Dis J. 1991 Sep;10(9):677–683. [PubMed] [Google Scholar]
- Henriksen S. D. Corroding bacteria from the respiratory tract. 1. Moraxella kingii. Acta Pathol Microbiol Scand. 1969;75(1):85–90. [PubMed] [Google Scholar]
- Jensen K. T., Schønheyder H., Thomsen V. F. In-vitro activity of beta-lactam and other antimicrobial agents against Kingella kingae. J Antimicrob Chemother. 1994 Mar;33(3):635–640. doi: 10.1093/jac/33.3.635. [DOI] [PubMed] [Google Scholar]
- Mirrett S., Reller L. B., Knapp J. S. Neisseria gonorrhoeae strains inhibited by vancomycin in selective media and correlation with auxotype. J Clin Microbiol. 1981 Jul;14(1):94–99. doi: 10.1128/jcm.14.1.94-99.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yagupsky P., Dagan R., Howard C. B., Einhorn M., Kassis I., Simu A. Clinical features and epidemiology of invasive Kingella kingae infections in southern Israel. Pediatrics. 1993 Dec;92(6):800–804. [PubMed] [Google Scholar]
- Yagupsky P., Dagan R., Howard C. W., Einhorn M., Kassis I., Simu A. High prevalence of Kingella kingae in joint fluid from children with septic arthritis revealed by the BACTEC blood culture system. J Clin Microbiol. 1992 May;30(5):1278–1281. doi: 10.1128/jcm.30.5.1278-1281.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- von Graevenitz A., Bucher C. Accuracy of the KOH and vancomycin tests in determining the Gram reaction of non-enterobacterial rods. J Clin Microbiol. 1983 Oct;18(4):983–985. doi: 10.1128/jcm.18.4.983-985.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]