Abstract
Currently recommended dilution test methods for the determination of antimicrobial susceptibility of Stenotrophomonas (Xanthomonas) maltophilia are labor-intensive and often impractical in many clinical laboratories. We compared the E test with the agar dilution method for susceptibility testing of 176 clinical isolates of S. maltophilia against 16 antimicrobial agents. The MICs obtained by E test correlated well with those determined by the agar dilution method, with an overall agreement of 94%. Very major and major errors occurred infrequently (0.6 to 2.9%) when testing beta-lactam agents, tobramycin, trimethoprim-sulfamethoxazole, and fluoroquinolones. The E test was found to be accurate and easy to perform. For most antimicrobial agents tested against S. maltophilia, the E test is an acceptable alternative susceptibility test method.
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- Baker C. N., Stocker S. A., Culver D. H., Thornsberry C. Comparison of the E Test to agar dilution, broth microdilution, and agar diffusion susceptibility testing techniques by using a special challenge set of bacteria. J Clin Microbiol. 1991 Mar;29(3):533–538. doi: 10.1128/jcm.29.3.533-538.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown D. F. The E-Test challenged with selected strains. Diagn Microbiol Infect Dis. 1992 Jul;15(5):465–468. doi: 10.1016/0732-8893(92)90091-7. [DOI] [PubMed] [Google Scholar]
- Hohl P., Frei R., Aubry P. In vitro susceptibility of 33 clinical case isolates of Xanthomonas maltophilia. Inconsistent correlation of agar dilution and of disk diffusion test results. Diagn Microbiol Infect Dis. 1991 Sep-Oct;14(5):447–450. doi: 10.1016/0732-8893(91)90072-n. [DOI] [PubMed] [Google Scholar]
- Marshall W. F., Keating M. R., Anhalt J. P., Steckelberg J. M. Xanthomonas maltophilia: an emerging nosocomial pathogen. Mayo Clin Proc. 1989 Sep;64(9):1097–1104. doi: 10.1016/s0025-6196(12)64979-9. [DOI] [PubMed] [Google Scholar]
- Morrison A. J., Jr, Hoffmann K. K., Wenzel R. P. Associated mortality and clinical characteristics of nosocomial Pseudomonas maltophilia in a university hospital. J Clin Microbiol. 1986 Jul;24(1):52–55. doi: 10.1128/jcm.24.1.52-55.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Muder R. R., Yu V. L., Dummer J. S., Vinson C., Lumish R. M. Infections caused by Pseudomonas maltophilia. Expanding clinical spectrum. Arch Intern Med. 1987 Sep;147(9):1672–1674. [PubMed] [Google Scholar]
- Neu H. C., Saha G., Chin N. X. Resistance of Xanthomonas maltophilia to antibiotics and the effect of beta-lactamase inhibitors. Diagn Microbiol Infect Dis. 1989 May-Jun;12(3):283–285. doi: 10.1016/0732-8893(89)90030-8. [DOI] [PubMed] [Google Scholar]
- Palleroni N. J., Bradbury J. F. Stenotrophomonas, a new bacterial genus for Xanthomonas maltophilia (Hugh 1980) Swings et al. 1983. Int J Syst Bacteriol. 1993 Jul;43(3):606–609. doi: 10.1099/00207713-43-3-606. [DOI] [PubMed] [Google Scholar]
- Pankuch G. A., Jacobs M. R., Rittenhouse S. F., Appelbaum P. C. Susceptibilities of 123 strains of Xanthomonas maltophilia to eight beta-lactams (including beta-lactam-beta-lactamase inhibitor combinations) and ciprofloxacin tested by five methods. Antimicrob Agents Chemother. 1994 Oct;38(10):2317–2322. doi: 10.1128/aac.38.10.2317. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vartivarian S., Anaissie E., Bodey G., Sprigg H., Rolston K. A changing pattern of susceptibility of Xanthomonas maltophilia to antimicrobial agents: implications for therapy. Antimicrob Agents Chemother. 1994 Mar;38(3):624–627. doi: 10.1128/aac.38.3.624. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Villarino M. E., Stevens L. E., Schable B., Mayers G., Miller J. M., Burke J. P., Jarvis W. R. Risk factors for epidemic Xanthomonas maltophilia infection/colonization in intensive care unit patients. Infect Control Hosp Epidemiol. 1992 Apr;13(4):201–206. doi: 10.1086/646510. [DOI] [PubMed] [Google Scholar]