Abstract
Four methods for the accurate delineation of epidemiologically related and unrelated strains of Candida lusitaniae were compared. Three pulsed-field electrophoretic methods, including two contour-clamped homogeneous field gel electrophoresis methods (EKP-1 and EKP-2) yielding electrophoretic karyotype patterns of intact chromosomal DNA and a method in which the chromosomal DNA was macrodigested with the endonuclease SfiI prior to pulsed-field electrophoresis (MDP), and a random amplified polymorphic DNA (RAPD) assay were evaluated. A selected panel of 21 well-characterized isolated representing 13 strains of C. lusitaniae, including 7 epidemiologically related isolates of one strain (group I-A), 3 epidemiologically related isolates of another strain (group I-B), and 11 epidemiologically unrelated isolates (group II), were tested. All isolates were coded and tested in a blinded manner. All seven group I-A isolates were confirmed to be a single strain by the EKP-1 and MDP methods, and the three group I-B isolates were shown to be a single strain by the EKP-1, EKP-2, MDP, and RAPD methods. Subtle differences were noted with two of the group I-A isolates by the EKP-2 method, whereas three of these isolates were different by the RAPD method. Each group II isolate had distinct patterns by all four methods. These data support the fact that the three pulsed-field electrophoretic methods and the RAPD method can be used to delineate strains of C. lusitaniae.(ABSTRACT TRUNCATED AT 250 WORDS)
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- Blinkhorn R. J., Adelstein D., Spagnuolo P. J. Emergence of a new opportunistic pathogen, Candida lusitaniae. J Clin Microbiol. 1989 Feb;27(2):236–240. doi: 10.1128/jcm.27.2.236-240.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chu G., Vollrath D., Davis R. W. Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science. 1986 Dec 19;234(4783):1582–1585. doi: 10.1126/science.3538420. [DOI] [PubMed] [Google Scholar]
- Guinet R., Chanas J., Goullier A., Bonnefoy G., Ambroise-Thomas P. Fatal septicemia due to amphotericin B-resistant Candida lusitaniae. J Clin Microbiol. 1983 Aug;18(2):443–444. doi: 10.1128/jcm.18.2.443-444.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hadfield T. L., Smith M. B., Winn R. E., Rinaldi M. G., Guerra C. Mycoses caused by Candida lusitaniae. Rev Infect Dis. 1987 Sep-Oct;9(5):1006–1012. doi: 10.1093/clinids/9.5.1006. [DOI] [PubMed] [Google Scholar]
- Holzschu D. L., Presley H. L., Miranda M., Phaff H. J. Identification of Candida lusitaniae as an opportunistic yeast in humans. J Clin Microbiol. 1979 Aug;10(2):202–205. doi: 10.1128/jcm.10.2.202-205.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kersulyte D., Woods J. P., Keath E. J., Goldman W. E., Berg D. E. Diversity among clinical isolates of Histoplasma capsulatum detected by polymerase chain reaction with arbitrary primers. J Bacteriol. 1992 Nov;174(22):7075–7079. doi: 10.1128/jb.174.22.7075-7079.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lehmann P. F., Lin D., Lasker B. A. Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA. J Clin Microbiol. 1992 Dec;30(12):3249–3254. doi: 10.1128/jcm.30.12.3249-3254.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Libertin C. R., Wilson W. R., Roberts G. D. Candida lusitaniae--an opportunistic pathogen. Diagn Microbiol Infect Dis. 1985 Jan;3(1):69–71. doi: 10.1016/0732-8893(85)90069-0. [DOI] [PubMed] [Google Scholar]
- Merz W. G. Candida lusitaniae: frequency of recovery, colonization, infection, and amphotericin B resistance. J Clin Microbiol. 1984 Dec;20(6):1194–1195. doi: 10.1128/jcm.20.6.1194-1195.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Merz W. G., Khazan U., Jabra-Rizk M. A., Wu L. C., Osterhout G. J., Lehmann P. F. Strain delineation and epidemiology of Candida (Clavispora) lusitaniae. J Clin Microbiol. 1992 Feb;30(2):449–454. doi: 10.1128/jcm.30.2.449-454.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Merz W. G., Sandford G. R. Isolation and characterization of a polyene-resistant variant of Candida tropicalis. J Clin Microbiol. 1979 Jun;9(6):677–680. doi: 10.1128/jcm.9.6.677-680.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pappagianis D., Collins M. S., Hector R., Remington J. Development of resistance to amphotericin B in Candida lusitaniae infecting a human. Antimicrob Agents Chemother. 1979 Aug;16(2):123–126. doi: 10.1128/aac.16.2.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanchez V., Vazquez J. A., Barth-Jones D., Dembry L., Sobel J. D., Zervos M. J. Epidemiology of nosocomial acquisition of Candida lusitaniae. J Clin Microbiol. 1992 Nov;30(11):3005–3008. doi: 10.1128/jcm.30.11.3005-3008.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vazquez J. A., Beckley A., Donabedian S., Sobel J. D., Zervos M. J. Comparison of restriction enzyme analysis versus pulsed-field gradient gel electrophoresis as a typing system for Torulopsis glabrata and Candida species other than C. albicans. J Clin Microbiol. 1993 Aug;31(8):2021–2030. doi: 10.1128/jcm.31.8.2021-2030.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woods J. P., Kersulyte D., Goldman W. E., Berg D. E. Fast DNA isolation from Histoplasma capsulatum: methodology for arbitrary primer polymerase chain reaction-based epidemiological and clinical studies. J Clin Microbiol. 1993 Feb;31(2):463–464. doi: 10.1128/jcm.31.2.463-464.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]