Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1995 Jun;33(6):1567–1572. doi: 10.1128/jcm.33.6.1567-1572.1995

Clinical and epidemiological investigations of Acinetobacter genomospecies 3 in a neonatal intensive care unit.

A Horrevorts 1, K Bergman 1, L Kollée 1, I Breuker 1, I Tjernberg 1, L Dijkshoorn 1
PMCID: PMC228217  PMID: 7650188

Abstract

A prospective study of Acinetobacter isolates from a neonatal intensive care unit was performed for 24 months. Fifty-six isolates were obtained from 21 patients, and another eight were obtained from environmental specimens. Infection due to Acinetobacter organisms was established for 16 patients, 6 with septicemia, 9 with pneumonia, and 1 with a wound infection. Further investigations were performed with 38 representative isolates. Twenty-nine isolates were identified as unnamed DNA-DNA hybridization group (genomospecies) 3, three were identified as genomospecies 2 (Acinetobacter baumannii), one was identified as genomospecies 5 (Acinetobacter junii), three were identified as genomospecies 14, and two were unclassified. Eight distinguishable protein profiles, coded I through VIII, were found by cell envelope protein electrophoresis. Profile V, a common profile, was observed for 17 isolates that had been recovered from 11 patients and 1 dust specimen. These isolates, all of which belonged to genomospecies 3, had similar antibiograms and biotypes. This study has revealed that genomospecies 3 can be associated with infection and be spread in hospitals.

Full Text

The Full Text of this article is available as a PDF (351.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander M., Ismail F., Jackman P. J., Noble W. C. Fingerprinting Acinetobacter strains from clinical sources by numerical analysis of electrophoretic protein patterns. J Med Microbiol. 1984 Aug;18(1):55–64. doi: 10.1099/00222615-18-1-55. [DOI] [PubMed] [Google Scholar]
  2. Beck-Sagué C. M., Jarvis W. R., Brook J. H., Culver D. H., Potts A., Gay E., Shotts B. W., Hill B., Anderson R. L., Weinstein M. P. Epidemic bacteremia due to Acinetobacter baumannii in five intensive care units. Am J Epidemiol. 1990 Oct;132(4):723–733. doi: 10.1093/oxfordjournals.aje.a115714. [DOI] [PubMed] [Google Scholar]
  3. Bergogne-Bérézin E., Joly-Guillou M. L., Vieu J. F. Epidemiology of nosocomial infections due to Acinetobacter calcoaceticus. J Hosp Infect. 1987 Sep;10(2):105–113. doi: 10.1016/0195-6701(87)90135-6. [DOI] [PubMed] [Google Scholar]
  4. Bouvet P. J., Grimont P. A. Identification and biotyping of clinical isolates of Acinetobacter. Ann Inst Pasteur Microbiol. 1987 Sep-Oct;138(5):569–578. doi: 10.1016/0769-2609(87)90042-1. [DOI] [PubMed] [Google Scholar]
  5. Bouvet P. J., Jeanjean S. Delineation of new proteolytic genomic species in the genus Acinetobacter. Res Microbiol. 1989 May-Jun;140(4-5):291–299. doi: 10.1016/0923-2508(89)90021-1. [DOI] [PubMed] [Google Scholar]
  6. Bouvet P. J., Jeanjean S., Vieu J. F., Dijkshoorn L. Species, biotype, and bacteriophage type determinations compared with cell envelope protein profiles for typing Acinetobacter strains. J Clin Microbiol. 1990 Feb;28(2):170–176. doi: 10.1128/jcm.28.2.170-176.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Crombach W. H., Dijkshoorn L., van Noort-Klaassen M., Niessen J., van Knippenberg-Gordebeke G. Control of an epidemic spread of a multi-resistant strain of Acinetobacter calcoaceticus in a hospital. Intensive Care Med. 1989;15(3):166–170. doi: 10.1007/BF01058568. [DOI] [PubMed] [Google Scholar]
  8. Dijkshoorn L., Michel M. F., Degener J. E. Cell envelope protein profiles of Acinetobacter calcoaceticus strains isolated in hospitals. J Med Microbiol. 1987 Jun;23(4):313–319. doi: 10.1099/00222615-23-4-313. [DOI] [PubMed] [Google Scholar]
  9. Dijkshoorn L., Van Ooyen A., Hop W. C., Theuns M., Michel M. F. Comparison of clinical Acinetobacter strains using a carbon source growth assay. Epidemiol Infect. 1990 Jun;104(3):443–453. doi: 10.1017/s0950268800047452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dijkshoorn L., van Dalen R., van Ooyen A., Bijl D., Tjernberg I., Michel M. F., Horrevorts A. M. Endemic acinetobacter in intensive care units: epidemiology and clinical impact. J Clin Pathol. 1993 Jun;46(6):533–536. doi: 10.1136/jcp.46.6.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gerner-Smidt P. Ribotyping of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. J Clin Microbiol. 1992 Oct;30(10):2680–2685. doi: 10.1128/jcm.30.10.2680-2685.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gerner-Smidt P., Tjernberg I., Ursing J. Reliability of phenotypic tests for identification of Acinetobacter species. J Clin Microbiol. 1991 Feb;29(2):277–282. doi: 10.1128/jcm.29.2.277-282.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Glew R. H., Moellering R. C., Jr, Kunz L. J. Infections with Acinetobacter calcoaceticus (Herellea vaginicola): clinical and laboratory studies. Medicine (Baltimore) 1977 Mar;56(2):79–97. doi: 10.1097/00005792-197703000-00001. [DOI] [PubMed] [Google Scholar]
  14. Henriksen S. D. Moraxella, Acinetobacter, and the Mimeae. Bacteriol Rev. 1973 Dec;37(4):522–561. doi: 10.1128/br.37.4.522-561.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morgan M. E., Hart C. A. Acinetobacter meningitis: acquired infection in a neonatal intensive care unit. Arch Dis Child. 1982 Jul;57(7):557–559. doi: 10.1136/adc.57.7.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ng P. C., Herrington R. A., Beane C. A., Ghoneim A. T., Dear P. R. An outbreak of acinetobacter septicaemia in a neonatal intensive care unit. J Hosp Infect. 1989 Nov;14(4):363–368. doi: 10.1016/0195-6701(89)90077-7. [DOI] [PubMed] [Google Scholar]
  17. Sakata H., Fujita K., Maruyama S., Kakehashi H., Mori Y., Yoshioka H. Acinetobacter calcoaceticus biovar anitratus septicaemia in a neonatal intensive care unit: epidemiology and control. J Hosp Infect. 1989 Jul;14(1):15–22. doi: 10.1016/0195-6701(89)90129-1. [DOI] [PubMed] [Google Scholar]
  18. Schloesser R. L., Laufkoetter E. A., Lehners T., Mietens C. An outbreak of Acinetobacter calcoaceticus infection in a neonatal care unit. Infection. 1990 Jul-Aug;18(4):230–233. doi: 10.1007/BF01643394. [DOI] [PubMed] [Google Scholar]
  19. Seifert H., Schulze A., Baginski R., Pulverer G. Plasmid DNA fingerprinting of Acinetobacter species other than Acinetobacter baumannii. J Clin Microbiol. 1994 Jan;32(1):82–86. doi: 10.1128/jcm.32.1.82-86.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sherertz R. J., Sullivan M. L. An outbreak of infections with Acinetobacter calcoaceticus in burn patients: contamination of patients' mattresses. J Infect Dis. 1985 Feb;151(2):252–258. doi: 10.1093/infdis/151.2.252. [DOI] [PubMed] [Google Scholar]
  21. Siegman-Igra Y., Bar-Yosef S., Gorea A., Avram J. Nosocomial acinetobacter meningitis secondary to invasive procedures: report of 25 cases and review. Clin Infect Dis. 1993 Nov;17(5):843–849. doi: 10.1093/clinids/17.5.843. [DOI] [PubMed] [Google Scholar]
  22. Stone J. W., Das B. C. Investigation of an outbreak of infection with Acinetobacter calcoaceticus in a special care baby unit. J Hosp Infect. 1986 Jan;7(1):42–48. doi: 10.1016/0195-6701(86)90025-3. [DOI] [PubMed] [Google Scholar]
  23. Tjernberg I., Ursing J. Clinical strains of Acinetobacter classified by DNA-DNA hybridization. APMIS. 1989 Jul;97(7):595–605. doi: 10.1111/j.1699-0463.1989.tb00449.x. [DOI] [PubMed] [Google Scholar]
  24. Traub W. H. Acinetobacter baumannii serotyping for delineation of outbreaks of nosocomial cross-infection. J Clin Microbiol. 1989 Dec;27(12):2713–2716. doi: 10.1128/jcm.27.12.2713-2716.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vila J., Almela M., Jimenez de Anta M. T. Laboratory investigation of hospital outbreak caused by two different multiresistant Acinetobacter calcoaceticus subsp. anitratus strains. J Clin Microbiol. 1989 May;27(5):1086–1089. doi: 10.1128/jcm.27.5.1086-1089.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Weaver R. E., Actis L. A. Identification of Acinetobacter species. J Clin Microbiol. 1994 Jul;32(7):1833–1833. doi: 10.1128/jcm.32.7.1833-.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES