Abstract
A selective medium containing mupirocin and metronidazole was evaluated for the isolation of Actinomyces spp. from clinical material. The study was a one-year prospective comparison of the method with existing methods, which use nonselective media, at a general hospital and a dental hospital. Significantly more Actinomyces spp. were isolated on the selective medium than on nonselective media from both dental specimens and intrauterine contraceptive devices. However, differentiating between Actinomyces spp. and related nonsporulating gram-positive rods remains a slow and sometimes uncertain process which can introduce lengthy delays in reporting.
Full Text
The Full Text of this article is available as a PDF (215.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Appelbaum P. C., Chatterton S. A. Susceptibility of anaerobic bacteria to ten antimicrobial agents. Antimicrob Agents Chemother. 1978 Sep;14(3):371–376. doi: 10.1128/aac.14.3.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beighton D., Colman G. A medium for the isolation and enumeration of oral actinomycetaceae from dental plaque. J Dent Res. 1976 Sep-Oct;55(5):875–878. doi: 10.1177/00220345760550052701. [DOI] [PubMed] [Google Scholar]
- Dubreuil L., Devos J., Neut C., Romond C. Susceptibility of anaerobic bacteria from several French hospitals to three major antibiotics. Antimicrob Agents Chemother. 1984 Jun;25(6):764–766. doi: 10.1128/aac.25.6.764. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldstein E. J., Sutter V. L., Finegold S. M. Comparative susceptibilities of anaerobic bacteria to metronidazole, ornidazole, and SC-28538. Antimicrob Agents Chemother. 1978 Oct;14(4):609–613. doi: 10.1128/aac.14.4.609. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hardie J. M. Oral microbiology: current concepts in the microbiology of dental caries and periodontal disease. Br Dent J. 1992 Apr 11;172(7):271–278. doi: 10.1038/sj.bdj.4807849. [DOI] [PubMed] [Google Scholar]
- Jarvis D. Isolation and identification of actinomycetes from women using intrauterine contraceptive devices. J Infect. 1985 Mar;10(2):121–125. doi: 10.1016/s0163-4453(85)91543-9. [DOI] [PubMed] [Google Scholar]
- Kilian M. Rapid identification of Actinomycetaceae and related bacteria. J Clin Microbiol. 1978 Aug;8(2):127–133. doi: 10.1128/jcm.8.2.127-133.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kornman K. S., Loesche W. J. New medium for isolation of Actinomyces viscosus and Actinomyces naeslundii from dental plaque. J Clin Microbiol. 1978 Jun;7(6):514–518. doi: 10.1128/jcm.7.6.514-518.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Lewis M. A., MacFarlane T. W., McGowan D. A. A microbiological and clinical review of the acute dentoalveolar abscess. Br J Oral Maxillofac Surg. 1990 Dec;28(6):359–366. doi: 10.1016/0266-4356(90)90032-g. [DOI] [PubMed] [Google Scholar]
- Lewis M. A., MacFarlane T. W., McGowan D. A. Quantitative bacteriology of acute dento-alveolar abscesses. J Med Microbiol. 1986 Mar;21(2):101–104. doi: 10.1099/00222615-21-2-101. [DOI] [PubMed] [Google Scholar]
- Traynor R. M., Parratt D., Duguid H. L., Duncan I. D. Isolation of actinomycetes from cervical specimens. J Clin Pathol. 1981 Aug;34(8):914–916. doi: 10.1136/jcp.34.8.914. [DOI] [PMC free article] [PubMed] [Google Scholar]