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. 1995 Jun;33(6):1670–1673. doi: 10.1128/jcm.33.6.1670-1673.1995

Quantitation of human immunodeficiency virus type 1 DNA and RNA by a novel internally controlled PCR assay.

P Gupta 1, M Ding 1, M Cottrill 1, C Rinaldo 1, L Kingsley 1, S Wolinsky 1, J Mellors 1
PMCID: PMC228244  PMID: 7650213

Abstract

A novel internally controlled PCR (ICPCR) assay was developed to accurately quantitate human immunodeficiency virus type 1 (HIV-1) DNA and RNA in peripheral blood mononuclear cells and plasma. The ICPCR assay was sensitive and reproducible within a linear range of amplification of 10(0) to 10(3) copies for HIV-1 DNA and 10(1) to 10(4) copies for HIV-1 RNA. The assay detected HIV-1 RNA in plasma and peripheral blood mononuclear cells from all HIV-1 subjects regardless of disease stage. ICPCR was compared with a branched-DNA signal amplification assay for subjects beginning antiretroviral therapy. The reductions in plasma HIV-1 RNA in response to therapy were comparable with the two assays. The ICPCR assay should be useful in monitoring HIV-1 RNA levels both in natural history studies and in clinical trials of antiretroviral agents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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