Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1995 Jul;33(7):1779–1783. doi: 10.1128/jcm.33.7.1779-1783.1995

Analysis of clonal relationships among isolates of Shigella sonnei by different molecular typing methods.

P Y Liu 1, Y J Lau 1, B S Hu 1, J M Shyr 1, Z Y Shi 1, W S Tsai 1, Y H Lin 1, C Y Tseng 1
PMCID: PMC228268  PMID: 7545179

Abstract

Shigella sonnei is a major cause of diarrheal disease in developed as well as in developing countries. Epidemiologic studies of this organism have been limited by the lack of a simple and effective method for comparing strains. In this study, we have compared different molecular typing methods, i.e., plasmid profile analysis, restriction endonuclease analysis of plasmids, rRNA gene restriction analysis (ribotyping), pulsed-field gel electrophoresis (PFGE), and enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR (ERIC-PCR) for typing 20 clinical isolates of S. sonnei collected from six incidents of infection. PFGE and ERIC-PCR fingerprintings had the highest discriminatory power for discrimination of epidemiologically related isolates from epidemiologically unrelated strains of S. sonnei, and both gave seven distinct strain types among these isolates and the type strain of the species. Plasmid study and ribotyping produced only six and typing techniques demonstrated two distinct patterns, respectively, among these strains. All of these molecular an identical fingerprint for eight temporally related sporadic isolates. It is possible that these temporally related isolates belonged to a single bacterial clone and circulated obscurely through the community. Our results indicate that the ERIC-PCR technique represents a rapid and simple means for typing S. sonnei with a level of discrimination equivalent to that of PFGE but greater than those of plasmid profile analysis, restriction endonuclease analysis of plasmids, and ribotyping.

Full Text

The Full Text of this article is available as a PDF (464.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ellsworth D. L., Rittenhouse K. D., Honeycutt R. L. Artifactual variation in randomly amplified polymorphic DNA banding patterns. Biotechniques. 1993 Feb;14(2):214–217. [PubMed] [Google Scholar]
  2. Haertl R., Bandlow G. Epidemiological fingerprinting of Enterobacter cloacae by small-fragment restriction endonuclease analysis and pulsed-field gel electrophoresis of genomic restriction fragments. J Clin Microbiol. 1993 Jan;31(1):128–133. doi: 10.1128/jcm.31.1.128-133.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Haider K., Huq M. I., Samadi A. R., Ahmad K. Plasmid characterization of Shigella spp. isolated from children with shigellosis and asymptomatic excretors. J Antimicrob Chemother. 1985 Dec;16(6):691–698. doi: 10.1093/jac/16.6.691. [DOI] [PubMed] [Google Scholar]
  4. Hinojosa-Ahumada M., Swaminathan B., Hunter S. B., Cameron D. N., Kiehlbauch J. A., Wachsmuth I. K., Strockbine N. A. Restriction fragment length polymorphisms in rRNA operons for subtyping Shigella sonnei. J Clin Microbiol. 1991 Nov;29(11):2380–2384. doi: 10.1128/jcm.29.11.2380-2384.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Litwin C. M., Ryan K. J., Chipowsky S., Storm A., McCombie S. Molecular epidemiology of Shigella sonnei in Pima County, Arizona: evidence for a Mexico-related plasmid. J Infect Dis. 1990 Apr;161(4):797–800. doi: 10.1093/infdis/161.4.797. [DOI] [PubMed] [Google Scholar]
  6. Litwin C. M., Storm A. L., Chipowsky S., Ryan K. J. Molecular epidemiology of Shigella infections: plasmid profiles, serotype correlation, and restriction endonuclease analysis. J Clin Microbiol. 1991 Jan;29(1):104–108. doi: 10.1128/jcm.29.1.104-108.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Liu P. Y., Lau Y. J., Hu B. S., Shir J. M., Cheung M. H., Shi Z. Y., Tsai W. S. Use of PCR to study epidemiology of Serratia marcescens isolates in nosocomial infection. J Clin Microbiol. 1994 Aug;32(8):1935–1938. doi: 10.1128/jcm.32.8.1935-1938.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nastasi A., Pignato S., Mammina C., Giammanco G. rRNA gene restriction patterns and biotypes of Shigella sonnei. Epidemiol Infect. 1993 Feb;110(1):23–30. doi: 10.1017/s0950268800050640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Prado D., Murray B. E., Cleary T. G., Pickering L. K. Limitations of using the plasmid pattern as an epidemiological tool for clinical isolates of Shigella sonnei. J Infect Dis. 1987 Feb;155(2):314–316. doi: 10.1093/infdis/155.2.314. [DOI] [PubMed] [Google Scholar]
  10. Pruneda R. C., Farmer J. J., 3rd Bacteriophage typing of Shigella sonnei. J Clin Microbiol. 1977 Jan;5(1):66–74. doi: 10.1128/jcm.5.1.66-74.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Salvi R. J., Ahroon W., Saunders S. S., Arnold S. A. Evoked potentials: computer-automated threshold-tracking procedure using an objective detection criterion. Ear Hear. 1987 Jun;8(3):151–156. [PubMed] [Google Scholar]
  12. Soldati L., Piffaretti J. C. Molecular typing of Shigella strains using pulsed field gel electrophoresis and genome hybridization with insertion sequences. Res Microbiol. 1991 Jun;142(5):489–498. doi: 10.1016/0923-2508(91)90182-a. [DOI] [PubMed] [Google Scholar]
  13. Struelens M. J., Carlier E., Maes N., Serruys E., Quint W. G., van Belkum A. Nosocomial colonization and infection with multiresistant Acinetobacter baumannii: outbreak delineation using DNA macrorestriction analysis and PCR-fingerprinting. J Hosp Infect. 1993 Sep;25(1):15–32. doi: 10.1016/0195-6701(93)90005-k. [DOI] [PubMed] [Google Scholar]
  14. Szturm-Rubinsten S. Determination of biotype, phage type and colicinogenic character of Shigella sonnei, and its epidemiologic importance. Arch Immunol Ther Exp (Warsz) 1968;16(3):421–428. [PubMed] [Google Scholar]
  15. Tacket C. O., Cohen M. L. Shigellosis in day care centers: use of plasmid analysis to assess control measures. Pediatr Infect Dis. 1983 Mar-Apr;2(2):127–130. [PubMed] [Google Scholar]
  16. Tacket C. O., Shahid N., Huq M. I., Alim A. R., Cohen M. L. Usefulness of plasmid profiles for differentiation of Shigella isolates in Bangladesh. J Clin Microbiol. 1984 Aug;20(2):300–301. doi: 10.1128/jcm.20.2.300-301.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tietze E., Tschäpe H., Horn G., Laue F. Clonal distribution of multiple-drug-resistant Shigella sonnei strains: identification by means of plasmid pattern analysis. Ann Microbiol (Paris) 1984 Sep-Oct;135B(2):155–164. doi: 10.1016/s0769-2609(84)80023-x. [DOI] [PubMed] [Google Scholar]
  18. Versalovic J., Koeuth T., Lupski J. R. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 1991 Dec 25;19(24):6823–6831. doi: 10.1093/nar/19.24.6823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vlajinac H., Krajinovic S. Colicine production as an epidemiological marker for Shigella sonnei. J Hyg (Lond) 1983 Oct;91(2):273–276. doi: 10.1017/s0022172400060289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 1990 Dec 25;18(24):7213–7218. doi: 10.1093/nar/18.24.7213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yagupsky P., Loeffelholz M., Bell K., Menegus M. A. Use of multiple markers for investigation of an epidemic of Shigella sonnei infections in Monroe County, New York. J Clin Microbiol. 1991 Dec;29(12):2850–2855. doi: 10.1128/jcm.29.12.2850-2855.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. van Belkum A., Bax R., Peerbooms P., Goessens W. H., van Leeuwen N., Quint W. G. Comparison of phage typing and DNA fingerprinting by polymerase chain reaction for discrimination of methicillin-resistant Staphylococcus aureus strains. J Clin Microbiol. 1993 Apr;31(4):798–803. doi: 10.1128/jcm.31.4.798-803.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES