Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1995 Jul;33(7):1912–1914. doi: 10.1128/jcm.33.7.1912-1914.1995

Sandwich enzyme-linked immunosorbent assay compared with Pastorex latex agglutination test for diagnosing invasive aspergillosis in immunocompromised patients.

P E Verweij 1, D Stynen 1, A J Rijs 1, B E de Pauw 1, J A Hoogkamp-Korstanje 1, J F Meis 1
PMCID: PMC228297  PMID: 7665670

Abstract

The performance of a direct sandwich enzyme-linked immunosorbent assay (ELISA) for detecting Aspergillus galactomannan was compared with that of the Pastorex Aspergillus antigen latex agglutination (LA) test by using 532 serum samples from 61 patients at risk for invasive aspergillosis. The ELISA gave positive results earlier in the course of infection than did the LA test. A sensitivity of 70% and a specificity of 86% were obtained for the LA test and corresponding values of 90 and 84% were obtained for the ELISA when a series of serum samples was employed.

Full Text

The Full Text of this article is available as a PDF (154.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ansorg R., Heintschel von Heinegg E., Rath P. M. Aspergillus antigenuria compared to antigenemia in bone marrow transplant recipients. Eur J Clin Microbiol Infect Dis. 1994 Jul;13(7):582–589. doi: 10.1007/BF01971310. [DOI] [PubMed] [Google Scholar]
  2. Bodey G. P., Vartivarian S. Aspergillosis. Eur J Clin Microbiol Infect Dis. 1989 May;8(5):413–437. doi: 10.1007/BF01964057. [DOI] [PubMed] [Google Scholar]
  3. Haynes K., Rogers T. R. Retrospective evaluation of a latex agglutination test for diagnosis of invasive aspergillosis in immunocompromised patients. Eur J Clin Microbiol Infect Dis. 1994 Aug;13(8):670–674. doi: 10.1007/BF01973998. [DOI] [PubMed] [Google Scholar]
  4. Hopwood V., Johnson E. M., Cornish J. M., Foot A. B., Evans E. G., Warnock D. W. Use of the Pastorex aspergillus antigen latex agglutination test for the diagnosis of invasive aspergillosis. J Clin Pathol. 1995 Mar;48(3):210–213. doi: 10.1136/jcp.48.3.210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Knight F., Mackenzie D. W. Aspergillus antigen latex test for diagnosis of invasive aspergillosis. Lancet. 1992 Jan 18;339(8786):188–188. doi: 10.1016/0140-6736(92)90261-z. [DOI] [PubMed] [Google Scholar]
  6. Kurup V. P., Kumar A. Immunodiagnosis of aspergillosis. Clin Microbiol Rev. 1991 Oct;4(4):439–456. doi: 10.1128/cmr.4.4.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Manso E., Montillo M., De Sio G., D'Amico S., Discepoli G., Leoni P. Value of antigen and antibody detection in the serological diagnosis of invasive aspergillosis in patients with hematological malignancies. Eur J Clin Microbiol Infect Dis. 1994 Sep;13(9):756–760. doi: 10.1007/BF02276061. [DOI] [PubMed] [Google Scholar]
  8. Melchers W. J., Verweij P. E., van den Hurk P., van Belkum A., De Pauw B. E., Hoogkamp-Korstanje J. A., Meis J. F. General primer-mediated PCR for detection of Aspergillus species. J Clin Microbiol. 1994 Jul;32(7):1710–1717. doi: 10.1128/jcm.32.7.1710-1717.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nalesnik M. A., Myerowitz R. L., Jenkins R., Lenkey J., Herbert D. Significance of Aspergillus species isolated from respiratory secretions in the diagnosis of invasive pulmonary aspergillosis. J Clin Microbiol. 1980 Apr;11(4):370–376. doi: 10.1128/jcm.11.4.370-376.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Reddy L. V., Kumar A., Kurup V. P. Specific amplification of Aspergillus fumigatus DNA by polymerase chain reaction. Mol Cell Probes. 1993 Apr;7(2):121–126. doi: 10.1006/mcpr.1993.1016. [DOI] [PubMed] [Google Scholar]
  11. Reiss E., Stockman L., Kuykendall R. J., Smith S. J. Dissociation of mannan--serum complexes and detection of Candida albicans mannan by enzyme immunoassay variations. Clin Chem. 1982 Feb;28(2):306–310. [PubMed] [Google Scholar]
  12. Spreadbury C., Holden D., Aufauvre-Brown A., Bainbridge B., Cohen J. Detection of Aspergillus fumigatus by polymerase chain reaction. J Clin Microbiol. 1993 Mar;31(3):615–621. doi: 10.1128/jcm.31.3.615-621.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stynen D., Goris A., Sarfati J., Latgé J. P. A new sensitive sandwich enzyme-linked immunosorbent assay to detect galactofuran in patients with invasive aspergillosis. J Clin Microbiol. 1995 Feb;33(2):497–500. doi: 10.1128/jcm.33.2.497-500.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stynen D., Sarfati J., Goris A., Prévost M. C., Lesourd M., Kamphuis H., Darras V., Latgé J. P. Rat monoclonal antibodies against Aspergillus galactomannan. Infect Immun. 1992 Jun;60(6):2237–2245. doi: 10.1128/iai.60.6.2237-2245.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tang C. M., Holden D. W., Aufauvre-Brown A., Cohen J. The detection of Aspergillus spp. by the polymerase chain reaction and its evaluation in bronchoalveolar lavage fluid. Am Rev Respir Dis. 1993 Nov;148(5):1313–1317. doi: 10.1164/ajrccm/148.5.1313. [DOI] [PubMed] [Google Scholar]
  16. Verweij P. E., Rijs A. J., De Pauw B. E., Horrevorts A. M., Hoogkamp-Korstanje J. A., Meis J. F. Clinical evaluation and reproducibility of the Pastorex Aspergillus antigen latex agglutination test for diagnosing invasive aspergillosis. J Clin Pathol. 1995 May;48(5):474–476. doi: 10.1136/jcp.48.5.474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Warnock D. W., Foot A. B., Johnson E. M., Mitchell S. B., Cornish J. M., Oakhill A. Aspergillus antigen latex test for diagnosis of invasive aspergillosis. Lancet. 1991 Oct 19;338(8773):1023–1024. doi: 10.1016/0140-6736(91)91890-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES