Abstract
A small polypeptide from pUL57 of human cytomegalovirus was identified as a major target for the immunoglobulin M antibody response. This antigen seems to be superior to antigenic fragments from pp150 and p52 in the identification of sera from acutely infected patients. It may therefore represent an essential antigen for recombinant immunoglobulin M antibody tests for human cytomegalovirus.
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- Albrecht J. C., Fleckenstein B. Structural organization of the conserved gene block of Herpesvirus saimiri coding for DNA polymerase, glycoprotein B, and major DNA binding protein. Virology. 1990 Feb;174(2):533–542. doi: 10.1016/0042-6822(90)90107-3. [DOI] [PubMed] [Google Scholar]
- Balfour H. H., Jr Management of cytomegalovirus disease with antiviral drugs. Rev Infect Dis. 1990 Sep-Oct;12 (Suppl 7):S849–S860. doi: 10.1093/clinids/12.supplement_7.s849. [DOI] [PubMed] [Google Scholar]
- Chee M. S., Bankier A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsnell T., Hutchison C. A., 3rd, Kouzarides T., Martignetti J. A. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr Top Microbiol Immunol. 1990;154:125–169. doi: 10.1007/978-3-642-74980-3_6. [DOI] [PubMed] [Google Scholar]
- Gorgievski-Hrisoho M., Hinderer W., Nebel-Schickel H., Horn J., Vornhagen R., Sonneborn H. H., Wolf H., Siegl G. Serodiagnosis of infectious mononucleosis by using recombinant Epstein-Barr virus antigens and enzyme-linked immunosorbent assay technology. J Clin Microbiol. 1990 Oct;28(10):2305–2311. doi: 10.1128/jcm.28.10.2305-2311.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landini M. P., Guan M. X., Jahn G., Lindenmaier W., Mach M., Ripalti A., Necker A., Lazzarotto T., Plachter B. Large-scale screening of human sera with cytomegalovirus recombinant antigens. J Clin Microbiol. 1990 Jun;28(6):1375–1379. doi: 10.1128/jcm.28.6.1375-1379.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lazzarotto T., Dalla Casa B., Campisi B., Landini M. P. Enzyme-linked immunoadsorbent assay for the detection of cytomegalovirus-IgM: comparison between eight commercial kits, immunofluorescence, and immunoblotting. J Clin Lab Anal. 1992;6(4):216–218. doi: 10.1002/jcla.1860060409. [DOI] [PubMed] [Google Scholar]
- Motz M., Fan J., Seibl R., Jilg W., Wolf H. Expression of the Epstein-Barr virus 138-kDa early protein in Escherichia coli for the use as antigen in diagnostic tests. Gene. 1986;42(3):303–312. doi: 10.1016/0378-1119(86)90234-9. [DOI] [PubMed] [Google Scholar]
- Plachter B., Wieczorek L., Scholl B. C., Ziegelmaier R., Jahn G. Detection of cytomegalovirus antibodies by an enzyme-linked immunosorbent assay using recombinant polypeptides of the large phosphorylated tegument protein pp150. J Clin Microbiol. 1992 Jan;30(1):201–206. doi: 10.1128/jcm.30.1.201-206.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith D. B., Johnson K. S. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 1988 Jul 15;67(1):31–40. doi: 10.1016/0378-1119(88)90005-4. [DOI] [PubMed] [Google Scholar]
- Studier F. W., Rosenberg A. H., Dunn J. J., Dubendorff J. W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 1990;185:60–89. doi: 10.1016/0076-6879(90)85008-c. [DOI] [PubMed] [Google Scholar]
- The T. H., van der Bij W., van den Berg A. P., van der Giessen M., Weits J., Sprenger H. G., van Son W. J. Cytomegalovirus antigenemia. Rev Infect Dis. 1990 Sep-Oct;12 (Suppl 7):S734–S744. [PubMed] [Google Scholar]
- Van der Giessen M., van den Berg A. P., van der Bij W., Postma S., van Son W. J., The T. H. Quantitative measurement of cytomegalovirus-specific IgG and IgM antibodies in relation to cytomegalovirus antigenaemia and disease activity in kidney recipients with an active cytomegalovirus infection. Clin Exp Immunol. 1990 Apr;80(1):56–61. doi: 10.1111/j.1365-2249.1990.tb06441.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vornhagen R., Plachter B., Hinderer W., The T. H., Van Zanten J., Matter L., Schmidt C. A., Sonneborn H. H., Jahn G. Early serodiagnosis of acute human cytomegalovirus infection by enzyme-linked immunosorbent assay using recombinant antigens. J Clin Microbiol. 1994 Apr;32(4):981–986. doi: 10.1128/jcm.32.4.981-986.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]