Abstract
Sixty-six stool specimens were evaluated by the ProSpecTR Cryptosporidium rapid enzyme immunoassay (EIA) (Alexon, Sunnyvale, Calif.). Approximately 2 g of untreated stool suspended in buffer was filtered through membranes labelled with anti-Cryptosporidium-specific antigen antibody. Anti-Cryptosporidium-specific antigen antibody was labelled with biotin, horseradish peroxidase conjugated to streptavidin, and tetramethylbenzidine, and each labelled antibody was added in sequence to the membranes. Each membrane had a positive control and test area. EIA results were compared with those of the modified acid-fast procedure. Twenty-three specimens were positive by the initial acid-fast procedure and the EIA. Forty-two specimens were negative by the initial acid-fast test and the EIA. One specimen was negative by the initial acid-fast test and positive by the EIA (sensitivity, 100%; specificity, 98.5%). This technique is easy to use by comparison with the cumbersome, labor-intensive, and more subjective microscopic methods currently available, and its sensitivity equals that of current microscopic methods.
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Selected References
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- Garcia L. S., Bruckner D. A., Brewer T. C., Shimizu R. Y. Techniques for the recovery and identification of Cryptosporidium oocysts from stool specimens. J Clin Microbiol. 1983 Jul;18(1):185–190. doi: 10.1128/jcm.18.1.185-190.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laughon B. E., Druckman D. A., Vernon A., Quinn T. C., Polk B. F., Modlin J. F., Yolken R. H., Bartlett J. G. Prevalence of enteric pathogens in homosexual men with and without acquired immunodeficiency syndrome. Gastroenterology. 1988 Apr;94(4):984–993. doi: 10.1016/0016-5085(88)90557-4. [DOI] [PubMed] [Google Scholar]
- Ma P., Soave R. Three-step stool examination for cryptosporidiosis in 10 homosexual men with protracted watery diarrhea. J Infect Dis. 1983 May;147(5):824–828. doi: 10.1093/infdis/147.5.824. [DOI] [PubMed] [Google Scholar]
- MacPherson D. W., McQueen R. Cryptosporidiosis: multiattribute evaluation of six diagnostic methods. J Clin Microbiol. 1993 Feb;31(2):198–202. doi: 10.1128/jcm.31.2.198-202.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith P. D., Lane H. C., Gill V. J., Manischewitz J. F., Quinnan G. V., Fauci A. S., Masur H. Intestinal infections in patients with the acquired immunodeficiency syndrome (AIDS). Etiology and response to therapy. Ann Intern Med. 1988 Mar;108(3):328–333. doi: 10.7326/0003-4819-108-3-328. [DOI] [PubMed] [Google Scholar]
- Sterling C. R., Arrowood M. J. Detection of Cryptosporidium sp. infections using a direct immunofluorescent assay. Pediatr Infect Dis. 1986 Jan-Feb;5(1 Suppl):S139–S142. doi: 10.1097/00006454-198601001-00022. [DOI] [PubMed] [Google Scholar]
- Tzipori S. Cryptosporidiosis in animals and humans. Microbiol Rev. 1983 Mar;47(1):84–96. doi: 10.1128/mr.47.1.84-96.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]