Abstract
The presence of clamp connections on hyphae and the development of fruiting bodies in culture are primary characters which allow identification of the basidiomycete Schizophyllum commune in cases of human infection. The diagnostic problems presented by a nonclamped, nonfruiting isolate from a dense mass in the right upper lobe of the lung in a female with a past history of pulmonary tuberculosis and diabetes are described. Several features of the isolated fungus, including rapid growth rate and white, dense, cottony colonies, tolerance to the fungicide benomyl at a concentration of 10 micrograms/ml, and susceptibility to cycloheximide at 400 micrograms/ml, suggested that it might be a basidiomycete. Transmission electron microscopy showed the presence of a dolipore septum with perforate pore cap characteristic of fungi in the class Holobasidiomycetes. However, species identification remained elusive until compatibility tests with known single-basidiospore isolates confirmed the identification of the sterile lung isolate as S. commune. Sequence analysis of the 5' internal transcribed spacer region of ribosomal DNA further supported conspecificity.
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Selected References
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- Catalano P., Lawson W., Bottone E., Lebenger J. Basidiomycetous (mushroom) infection of the maxillary sinus. Otolaryngol Head Neck Surg. 1990 Feb;102(2):183–185. doi: 10.1177/019459989010200217. [DOI] [PubMed] [Google Scholar]
- Greer D. L. Basidiomycetes as agents of human infections: a review. Mycopathologia. 1978 Dec 18;65(1-3):133–139. doi: 10.1007/BF00447184. [DOI] [PubMed] [Google Scholar]
- KLIGMAN A. M. A basidiomycete probably causing onychomycosis. J Invest Dermatol. 1950 Jan;14(1):67-70, illust. doi: 10.1038/jid.1950.10. [DOI] [PubMed] [Google Scholar]
- Kamei K., Unno H., Nagao K., Kuriyama T., Nishimura K., Miyaji M. Allergic bronchopulmonary mycosis caused by the basidiomycetous fungus Schizophyllum commune. Clin Infect Dis. 1994 Mar;18(3):305–309. doi: 10.1093/clinids/18.3.305. [DOI] [PubMed] [Google Scholar]
- Kern M. E., Uecker F. A. Maxillary sinus infection caused by the homobasidiomycetous fungus Schizophyllum commune. J Clin Microbiol. 1986 Jun;23(6):1001–1005. doi: 10.1128/jcm.23.6.1001-1005.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Padhye A. A., Sekhon A. S., Carmichael J. W. Ascocarp production by Nannizzia and Arthroderma on keratinous and non-keratinous media. Sabouraudia. 1973 Jul;11(2):109–114. [PubMed] [Google Scholar]
- Restrepo A., Greer D. L., Robledo M., Osorio O., Mondragón H. Ulceration of the palate caused by a basidiomycete Schizophyllum commune. Sabouraudia. 1973 Nov;11(3):201–204. [PubMed] [Google Scholar]
- Rosenthal J., Katz R., DuBois D. B., Morrissey A., Machicao A. Chronic maxillary sinusitis associated with the mushroom Schizophyllum commune in a patient with AIDS. Clin Infect Dis. 1992 Jan;14(1):46–48. doi: 10.1093/clinids/14.1.46. [DOI] [PubMed] [Google Scholar]
- Summerbell R. C. The benomyl test as a fundamental diagnostic method for medical mycology. J Clin Microbiol. 1993 Mar;31(3):572–577. doi: 10.1128/jcm.31.3.572-577.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]