Abstract
CDC coryneform group A-3 and A-4 bacteria were defined by Hollis and Weaver in 1981, but their taxonomic position is still unclear. By using biochemical and chemotaxonomical methods, four clinical strains belonging to CDC coryneform groups A-3 (n = 2) and A-4 (n = 2) were studied and could be assigned to the genus Cellulomonas, resulting in the first description of Cellulomonas strains isolated from clinical specimens. CDC coryneform group A-3 and A-4 strains were compared with the type strains of the seven species constituting the genus Cellulomonas at present as well as with the closely related species Oerskovia turbata, Oerskovia xanthineolytica, and Jonesia denitrificans, but their biochemical patterns were not compatible with the patterns of any of those species. Almost the entire sequences of the 16S rRNA genes of one representative strain of both CDC taxa were determined, and comparative sequence analysis confirmed the placement of the CDC coryneform group A-3 and A-4 strains studied in the Cellulomonas-Oerskovia subbranch of the actinomycetes. Both CDC taxa exhibited > 99% base pair homology within their 16S rDNAs. On the basis of phenotypic and molecular data, we formally propose a new species, Cellulomonas hominis sp. nov., for the CDC coryneform group A-3 bacteria examined. The type strain is DSM 9581. The precise taxonomic status of the CDC coryneform group A-4 strains studied remains to be established by quantitative DNA-DNA hybridizations.
Full Text
The Full Text of this article is available as a PDF (289.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bernard K. A., Bellefeuille M., Ewan E. P. Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic gram-positive rods. J Clin Microbiol. 1991 Jan;29(1):83–89. doi: 10.1128/jcm.29.1.83-89.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chou S. P., Kasatiya S., Irvine N. Cellular fatty acid composition of Oerskovia species, CDC Coryneform groups A-3, A-4, A-5, Corynebacterium aquaticum, Listeria denitrificans and Brevibacterium acetylicum. Antonie Van Leeuwenhoek. 1990 Aug;58(2):115–119. doi: 10.1007/BF00422727. [DOI] [PubMed] [Google Scholar]
- Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fernández-Garayzábal J. F., Dominguez L., Pascual C., Jones D., Collins M. D. Phenotypic and phylogenetic characterization of some unknown coryneform bacteria isolated from bovine blood and milk: description of Sanguibacter gen.nov. Lett Appl Microbiol. 1995 Feb;20(2):69–75. doi: 10.1111/j.1472-765x.1995.tb01289.x. [DOI] [PubMed] [Google Scholar]
- Funke G., Falsen E., Barreau C. Primary identification of Microbacterium spp. encountered in clinical specimens as CDC coryneform group A-4 and A-5 bacteria. J Clin Microbiol. 1995 Jan;33(1):188–192. doi: 10.1128/jcm.33.1.188-192.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Funke G., von Graevenitz A., Weiss N. Primary identification of Aureobacterium spp. isolated from clinical specimens as "Corynebacterium aquaticum". J Clin Microbiol. 1994 Nov;32(11):2686–2691. doi: 10.1128/jcm.32.11.2686-2691.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hutson R. A., Thompson D. E., Collins M. D. Genetic interrelationships of saccharolytic Clostridium botulinum types B, E and F and related clostridia as revealed by small-subunit rRNA gene sequences. FEMS Microbiol Lett. 1993 Mar 15;108(1):103–110. doi: 10.1111/j.1574-6968.1993.tb06081.x. [DOI] [PubMed] [Google Scholar]
- Na'Was T. E., Hollis D. G., Moss C. W., Weaver R. E. Comparison of biochemical, morphologic, and chemical characteristics of Centers for Disease Control fermentative coryneform groups 1, 2, and A-4. J Clin Microbiol. 1987 Aug;25(8):1354–1358. doi: 10.1128/jcm.25.8.1354-1358.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Prauser H., Lechevalier M. P., Lechevalier H. Description of Oerskovia gen. n. to harbor Orskov's motile nocardia. Appl Microbiol. 1970 Mar;19(3):534–534. doi: 10.1128/am.19.3.534-534.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Von Graevenitz A., Osterhout G., Dick J. Grouping of some clinically relevant gram-positive rods by automated fatty acid analysis. Diagnostic implications. APMIS. 1991 Feb;99(2):147–154. doi: 10.1111/j.1699-0463.1991.tb05132.x. [DOI] [PubMed] [Google Scholar]