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. 1995 Aug;33(8):2107–2113. doi: 10.1128/jcm.33.8.2107-2113.1995

Strategy to detect and identify Bartonella species in routine clinical laboratory yields Bartonella henselae from human immunodeficiency virus-positive patient and unique Bartonella strain from his cat.

J E Clarridge 3rd 1, T J Raich 1, D Pirwani 1, B Simon 1, L Tsai 1, M C Rodriguez-Barradas 1, R Regnery 1, A Zollo 1, D C Jones 1, C Rambo 1
PMCID: PMC228344  PMID: 7559957

Abstract

We wished to develop a cost-effective, rapid strategy to detect and identify Bartonella species in the clinical laboratory and to determine the prevalence of Bartonella infection in the Houston veteran population. Bartonella colonies were identified by colony morphology, Gram stain, RapID ANA, repetitive extragenic palindromic-PCR (REP-PCR) and whole-cell fatty acid (CFA) analysis, and these methods were compared for their usefulness. A new test order for "Rochalimaea culture" (the genus Bartonella was previously known as the genus Rochalimaea) was instituted, and in addition, all blood specimens submitted for fungal culture (obtained in an isolator tube) were processed for Bartonella culture. Over a 16-month period we isolated Bartonella henselae from only 0.4% (2 of 533) of total cultures but from 1% (2 of 204) of human immunodeficiency virus-positive patients. After sufficient growth, identification of the Bartonella isolates to the species level could be obtained in 2 days. The REP-PCR allowed discrimination of all known species, whereas CFA analysis distinguished all except B. henselae and Bartonella quintana. The RapID ANA results failed to differentiate between B. henselae and B. quintana, and results for other species differed by only one or two tests. Blood obtained from a kitten which had been introduced into the household of one patient 2 months before the onset of fever yielded a Bartonella strain which was shown to be different from the strain from the patient and distinct from other Bartonella species by a combination of REP-PCR, CFA, and growth characteristics.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Anderson B., Sims K., Regnery R., Robinson L., Schmidt M. J., Goral S., Hager C., Edwards K. Detection of Rochalimaea henselae DNA in specimens from cat scratch disease patients by PCR. J Clin Microbiol. 1994 Apr;32(4):942–948. doi: 10.1128/jcm.32.4.942-948.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brenner D. J., O'Connor S. P., Winkler H. H., Steigerwalt A. G. Proposals to unify the genera Bartonella and Rochalimaea, with descriptions of Bartonella quintana comb. nov., Bartonella vinsonii comb. nov., Bartonella henselae comb. nov., and Bartonella elizabethae comb. nov., and to remove the family Bartonellaceae from the order Rickettsiales. Int J Syst Bacteriol. 1993 Oct;43(4):777–786. doi: 10.1099/00207713-43-4-777. [DOI] [PubMed] [Google Scholar]
  3. Daly J. S., Worthington M. G., Brenner D. J., Moss C. W., Hollis D. G., Weyant R. S., Steigerwalt A. G., Weaver R. E., Daneshvar M. I., O'Connor S. P. Rochalimaea elizabethae sp. nov. isolated from a patient with endocarditis. J Clin Microbiol. 1993 Apr;31(4):872–881. doi: 10.1128/jcm.31.4.872-881.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dolan M. J., Wong M. T., Regnery R. L., Jorgensen J. H., Garcia M., Peters J., Drehner D. Syndrome of Rochalimaea henselae adenitis suggesting cat scratch disease. Ann Intern Med. 1993 Mar 1;118(5):331–336. doi: 10.7326/0003-4819-118-5-199303010-00002. [DOI] [PubMed] [Google Scholar]
  5. Drancourt M., Raoult D. Proposed tests for the routine identification of Rochalimaea species. Eur J Clin Microbiol Infect Dis. 1993 Sep;12(9):710–713. doi: 10.1007/BF02009387. [DOI] [PubMed] [Google Scholar]
  6. Georghiou P. R., Doggett A. M., Kielhofner M. A., Stout J. E., Watson D. A., Lupski J. R., Hamill R. J. Molecular fingerprinting of Legionella species by repetitive element PCR. J Clin Microbiol. 1994 Dec;32(12):2989–2994. doi: 10.1128/jcm.32.12.2989-2994.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Koehler J. E., Glaser C. A., Tappero J. W. Rochalimaea henselae infection. A new zoonosis with the domestic cat as reservoir. JAMA. 1994 Feb 16;271(7):531–535. doi: 10.1001/jama.271.7.531. [DOI] [PubMed] [Google Scholar]
  8. Koehler J. E., Quinn F. D., Berger T. G., LeBoit P. E., Tappero J. W. Isolation of Rochalimaea species from cutaneous and osseous lesions of bacillary angiomatosis. N Engl J Med. 1992 Dec 3;327(23):1625–1631. doi: 10.1056/NEJM199212033272303. [DOI] [PubMed] [Google Scholar]
  9. Larson A. M., Dougherty M. J., Nowowiejski D. J., Welch D. F., Matar G. M., Swaminathan B., Coyle M. B. Detection of Bartonella (Rochalimaea) quintana by routine acridine orange staining of broth blood cultures. J Clin Microbiol. 1994 Jun;32(6):1492–1496. doi: 10.1128/jcm.32.6.1492-1496.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lucey D., Dolan M. J., Moss C. W., Garcia M., Hollis D. G., Wegner S., Morgan G., Almeida R., Leong D., Greisen K. S. Relapsing illness due to Rochalimaea henselae in immunocompetent hosts: implication for therapy and new epidemiological associations. Clin Infect Dis. 1992 Mar;14(3):683–688. doi: 10.1093/clinids/14.3.683. [DOI] [PubMed] [Google Scholar]
  11. Marullo S., Jaccard A., Roulot D., Mainguene C., Clauvel J. P. Identification of the Rochalimaea henselae 16S rRNA sequence in the liver of a French patient with bacillary peliosis hepatis. J Infect Dis. 1992 Dec;166(6):1462–1462. doi: 10.1093/infdis/166.6.1462-a. [DOI] [PubMed] [Google Scholar]
  12. Matar G. M., Swaminathan B., Hunter S. B., Slater L. N., Welch D. F. Polymerase chain reaction-based restriction fragment length polymorphism analysis of a fragment of the ribosomal operon from Rochalimaea species for subtyping. J Clin Microbiol. 1993 Jul;31(7):1730–1734. doi: 10.1128/jcm.31.7.1730-1734.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maurin M., Roux V., Stein A., Ferrier F., Viraben R., Raoult D. Isolation and characterization by immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western blot, restriction fragment length polymorphism-PCR, 16S rRNA gene sequencing, and pulsed-field gel electrophoresis of Rochalimaea quintana from a patient with bacillary angiomatosis. J Clin Microbiol. 1994 May;32(5):1166–1171. doi: 10.1128/jcm.32.5.1166-1171.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Perkocha L. A., Geaghan S. M., Yen T. S., Nishimura S. L., Chan S. P., Garcia-Kennedy R., Honda G., Stoloff A. C., Klein H. Z., Goldman R. L. Clinical and pathological features of bacillary peliosis hepatis in association with human immunodeficiency virus infection. N Engl J Med. 1990 Dec 6;323(23):1581–1586. doi: 10.1056/NEJM199012063232302. [DOI] [PubMed] [Google Scholar]
  15. Regnery R. L., Anderson B. E., Clarridge J. E., 3rd, Rodriguez-Barradas M. C., Jones D. C., Carr J. H. Characterization of a novel Rochalimaea species, R. henselae sp. nov., isolated from blood of a febrile, human immunodeficiency virus-positive patient. J Clin Microbiol. 1992 Feb;30(2):265–274. doi: 10.1128/jcm.30.2.265-274.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Regnery R. L., Olson J. G., Perkins B. A., Bibb W. Serological response to "Rochalimaea henselae" antigen in suspected cat-scratch disease. Lancet. 1992 Jun 13;339(8807):1443–1445. doi: 10.1016/0140-6736(92)92032-b. [DOI] [PubMed] [Google Scholar]
  17. Relman D. A., Loutit J. S., Schmidt T. M., Falkow S., Tompkins L. S. The agent of bacillary angiomatosis. An approach to the identification of uncultured pathogens. N Engl J Med. 1990 Dec 6;323(23):1573–1580. doi: 10.1056/NEJM199012063232301. [DOI] [PubMed] [Google Scholar]
  18. Rodriguez-Barradas M. C., Hamill R. J., Houston E. D., Georghiou P. R., Clarridge J. E., Regnery R. L., Koehler J. E. Genomic fingerprinting of Bartonella species by repetitive element PCR for distinguishing species and isolates. J Clin Microbiol. 1995 May;33(5):1089–1093. doi: 10.1128/jcm.33.5.1089-1093.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schwartzman W. A. Infections due to Rochalimaea: the expanding clinical spectrum. Clin Infect Dis. 1992 Dec;15(6):893–900. doi: 10.1093/clind/15.6.893. [DOI] [PubMed] [Google Scholar]
  20. Slater L. N., Welch D. F., Hensel D., Coody D. W. A newly recognized fastidious gram-negative pathogen as a cause of fever and bacteremia. N Engl J Med. 1990 Dec 6;323(23):1587–1593. doi: 10.1056/NEJM199012063232303. [DOI] [PubMed] [Google Scholar]
  21. Spach D. H., Callis K. P., Paauw D. S., Houze Y. B., Schoenknecht F. D., Welch D. F., Rosen H., Brenner D. J. Endocarditis caused by Rochalimaea quintana in a patient infected with human immunodeficiency virus. J Clin Microbiol. 1993 Mar;31(3):692–694. doi: 10.1128/jcm.31.3.692-694.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tappero J. W., Mohle-Boetani J., Koehler J. E., Swaminathan B., Berger T. G., LeBoit P. E., Smith L. L., Wenger J. D., Pinner R. W., Kemper C. A. The epidemiology of bacillary angiomatosis and bacillary peliosis. JAMA. 1993 Feb 10;269(6):770–775. [PubMed] [Google Scholar]
  23. Versalovic J., Koeuth T., Lupski J. R. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 1991 Dec 25;19(24):6823–6831. doi: 10.1093/nar/19.24.6823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Welch D. F., Hensel D. M., Pickett D. A., San Joaquin V. H., Robinson A., Slater L. N. Bacteremia due to Rochalimaea henselae in a child: practical identification of isolates in the clinical laboratory. J Clin Microbiol. 1993 Sep;31(9):2381–2386. doi: 10.1128/jcm.31.9.2381-2386.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Welch D. F., Pickett D. A., Slater L. N., Steigerwalt A. G., Brenner D. J. Rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis. J Clin Microbiol. 1992 Feb;30(2):275–280. doi: 10.1128/jcm.30.2.275-280.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Woods C. R., Versalovic J., Koeuth T., Lupski J. R. Whole-cell repetitive element sequence-based polymerase chain reaction allows rapid assessment of clonal relationships of bacterial isolates. J Clin Microbiol. 1993 Jul;31(7):1927–1931. doi: 10.1128/jcm.31.7.1927-1931.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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