Abstract
Antigenic diversity among the bovine viral diarrhea virus (BVDV) cytopathic strains 87-2552 (field isolate) and NADL and Singer (prototype strains) was demonstrated with monoclonal antibodies (MAbs) in enzyme-linked immunosorbent, immunofluorescence, virus neutralization, and immunoprecipitation assays. Two MAbs against BVDV strain 87-2552, designated D11 and B7, strongly neutralized this field strain and were specific for the 48-kDa glycoprotein of the virus. These two MAbs have different subisotypes, immunoglobulin G1 for D11 and immunoglobulin G3 for B7. MAbs against BVDV strains 87-2552 and NADL were specific for their respective strains in virus neutralization assays. The results indicated significant antigenic differences between BVDV strain 87-2552 and the NADL and Singer strains.
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- Bolin S. R. Control of bovine virus diarrhoea virus. Rev Sci Tech. 1990 Mar;9(1):163–171. doi: 10.20506/rst.9.1.483. [DOI] [PubMed] [Google Scholar]
- Bolin S. R., Littledike E. T., Ridpath J. F. Serologic detection and practical consequences of antigenic diversity among bovine viral diarrhea viruses in a vaccinated herd. Am J Vet Res. 1991 Jul;52(7):1033–1037. [PubMed] [Google Scholar]
- Bolin S., Moennig V., Kelso Gourley N. E., Ridpath J. Monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhea virus into groups. Brief report. Arch Virol. 1988;99(1-2):117–123. doi: 10.1007/BF01311029. [DOI] [PubMed] [Google Scholar]
- Boulanger D., Waxweiler S., Karelle L., Loncar M., Mignon B., Dubuisson J., Thiry E., Pastoret P. P. Characterization of monoclonal antibodies to bovine viral diarrhoea virus: evidence of a neutralizing activity against gp48 in the presence of goat anti-mouse immunoglobulin serum. J Gen Virol. 1991 May;72(Pt 5):1195–1198. doi: 10.1099/0022-1317-72-5-1195. [DOI] [PubMed] [Google Scholar]
- Collett M. S., Anderson D. K., Retzel E. Comparisons of the pestivirus bovine viral diarrhoea virus with members of the flaviviridae. J Gen Virol. 1988 Oct;69(Pt 10):2637–2643. doi: 10.1099/0022-1317-69-10-2637. [DOI] [PubMed] [Google Scholar]
- Collett M. S., Larson R., Belzer S. K., Retzel E. Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology. 1988 Jul;165(1):200–208. doi: 10.1016/0042-6822(88)90673-3. [DOI] [PubMed] [Google Scholar]
- Corapi W. V., Donis R. O., Dubovi E. J. Characterization of a panel of monoclonal antibodies and their use in the study of the antigenic diversity of bovine viral diarrhea virus. Am J Vet Res. 1990 Sep;51(9):1388–1394. [PubMed] [Google Scholar]
- Corapi W. V., Donis R. O., Dubovi E. J. Monoclonal antibody analyses of cytopathic and noncytopathic viruses from fatal bovine viral diarrhea virus infections. J Virol. 1988 Aug;62(8):2823–2827. doi: 10.1128/jvi.62.8.2823-2827.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donis R. O., Corapi W., Dubovi E. J. Neutralizing monoclonal antibodies to bovine viral diarrhoea virus bind to the 56K to 58K glycoprotein. J Gen Virol. 1988 Jan;69(Pt 1):77–86. doi: 10.1099/0022-1317-69-1-77. [DOI] [PubMed] [Google Scholar]
- Howard C. J., Brownlie J., Clarke M. C. Comparison by the neutralisation assay of pairs of non-cytopathogenic and cytopathogenic strains of bovine virus diarrhoea virus isolated from cases of mucosal disease. Vet Microbiol. 1987 Apr;13(4):361–369. doi: 10.1016/0378-1135(87)90067-8. [DOI] [PubMed] [Google Scholar]
- Kwang J., Littledike E. T., Donis R. O., Dubovi E. J. Recombinant polypeptide from the gp48 region of the bovine viral diarrhea virus (BVDV) detects serum antibodies in vaccinated and infected cattle. Vet Microbiol. 1992 Oct;32(3-4):281–292. doi: 10.1016/0378-1135(92)90151-i. [DOI] [PubMed] [Google Scholar]
- Magar R., Minocha H. C., Lecomte J. Bovine viral diarrhea virus proteins: heterogeneity of cytopathogenic and non-cytopathogenic strains and evidence of a 53K glycoprotein neutralization epitope. Vet Microbiol. 1988 Apr;16(4):303–314. doi: 10.1016/0378-1135(88)90012-0. [DOI] [PubMed] [Google Scholar]
- Magar R., Minocha H. C., Montpetit C., Carman P. S., Lecomte J. Typing of cytopathic and noncytopathic bovine viral diarrhea virus reference and Canadian field strains using a neutralizing monoclonal antibody. Can J Vet Res. 1988 Jan;52(1):42–45. [PMC free article] [PubMed] [Google Scholar]
- Orten D. J., Blecha F., Morrill J. L., Minocha H. C. Characterization of anti-idiotype reagents to bovine herpesvirus-1 monoclonal antibody. Vet Immunol Immunopathol. 1988 Dec;20(1):1–14. doi: 10.1016/0165-2427(88)90021-9. [DOI] [PubMed] [Google Scholar]
- Pocock D. H., Howard C. J., Clarke M. C., Brownlie J. Variation in the intracellular polypeptide profiles from different isolates of bovine virus diarrhoea virus. Arch Virol. 1987;94(1-2):43–53. doi: 10.1007/BF01313724. [DOI] [PubMed] [Google Scholar]
- Renard A., Schmetz D., Guiot C., Brown-Shimmer S., Dagenais L., Pastoret P. P., Dina D., Martial J. A. Molecular cloning of the bovine viral diarrhea virus genomic RNA. Ann Rech Vet. 1987;18(2):121–125. [PubMed] [Google Scholar]
- Westaway E. G., Brinton M. A., Gaidamovich SYa, Horzinek M. C., Igarashi A., Käriäinen L., Lvov D. K., Porterfield J. S., Russell P. K., Trent D. W. Togaviridae. Intervirology. 1985;24(3):125–139. doi: 10.1159/000149632. [DOI] [PubMed] [Google Scholar]
- Xue W., Blecha F., Minocha H. C. Antigenic variations in bovine viral diarrhea viruses detected by monoclonal antibodies. J Clin Microbiol. 1990 Aug;28(8):1688–1693. doi: 10.1128/jcm.28.8.1688-1693.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Xue W., Orten D. J., Abdelmagid O. Y., Rider M., Blecha F., Minocha H. C. Anti-idiotypic antibodies mimic bovine viral diarrhea virus antigen. Vet Microbiol. 1991 Nov;29(3-4):201–212. doi: 10.1016/0378-1135(91)90128-3. [DOI] [PubMed] [Google Scholar]