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. 1995 Aug;33(8):2166–2170. doi: 10.1128/jcm.33.8.2166-2170.1995

Comparative evaluation of microagglutination test and serum agglutination test as supplementary diagnostic methods for brucellosis.

M Baum 1, O Zamir 1, R Bergman-Rios 1, E Katz 1, Z Beider 1, A Cohen 1, M Banai 1
PMCID: PMC228357  PMID: 7559970

Abstract

The diagnosis of brucellosis in cattle and small ruminants requires the use of more than one serological test. The complement fixation test (CFT), the rose bengal test (RBT), and the serum agglutination test (SAT) are among the most useful tests for routine diagnosis. The microagglutination test (MAT) was developed as a simpler and more efficient test than the SAT. The relative efficacy of this test compared with that of the SAT was evaluated by using brucella-free sheep and goats prior to and after vaccination treatment. The specificities of the MAT and the SAT were 100%. Of the ewes and goats with a vaccination history, one ewe, expectedly a negative responder, had reactions in the MAT, the complement fixation test, and the rose bengal test but not in the SAT, suggesting a lower sensitivity of the SAT in this case. The calculated sensitivities of the MAT and the SAT were 93.9%. The agreement between MAT and SAT results from nonresponders was examined by using sera from unvaccinated lambs and kids (95.2% agreement), unvaccinated ewes and goats (84.4%), and ewes and goats with a vaccination history (43.9%). For the latter group higher levels of agglutination units were observed by the MAT than by the SAT in 51.5% of the samples. In testing sera from positive reactors after vaccination neither method was superior (MAT values were greater than SAT values for 23.5% of the samples, and MAT values were less than SAT values for 21.9% of the samples).(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Alton G. G., Maw J., Rogerson B. A., McPherson G. G. The serological diagnosis of bovine brucellosis: an evaluation of the complement fixation, serum agglutination and rose bengal tests. Aust Vet J. 1975 Feb;51(2):57–63. doi: 10.1111/j.1751-0813.1975.tb09404.x. [DOI] [PubMed] [Google Scholar]
  2. Bettelheim K. A., Maskill W. J., Pearce J. Comparison of standard tube and microagglutination techniques for determining Brucella antibodies. J Hyg (Lond) 1983 Feb;90(1):33–39. doi: 10.1017/s0022172400063804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blasco J. M., Garin-Bastuji B., Marin C. M., Gerbier G., Fanlo J., Jiménez de Bagués M. P., Cau C. Efficacy of different Rose Bengal and complement fixation antigens for the diagnosis of Brucella melitensis infection in sheep and goats. Vet Rec. 1994 Apr 16;134(16):415–420. doi: 10.1136/vr.134.16.415. [DOI] [PubMed] [Google Scholar]
  4. Brown S. L., Klein G. C., McKinney F. T., Jones W. L. Safranin O-stained antigen microagglutination test for detection of brucella antibodies. J Clin Microbiol. 1981 Feb;13(2):398–400. doi: 10.1128/jcm.13.2.398-400.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Corbel M. J. Identification of the immunoglobulin class active in the Rose Bengal plate test for bovine brucellosis. J Hyg (Lond) 1972 Dec;70(4):779–795. doi: 10.1017/s0022172400022622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Corbel M. J., Stuart F. A., Brewer R. A. Observations on serological cross-reactions between smooth Brucella species and organisms of other genera. Dev Biol Stand. 1984;56:341–348. [PubMed] [Google Scholar]
  7. Dohoo I. R., Wright P. F., Ruckerbauer G. M., Samagh B. S., Robertson F. J., Forbes L. B. A comparison of five serological tests for bovine brucellosis. Can J Vet Res. 1986 Oct;50(4):485–493. [PMC free article] [PubMed] [Google Scholar]
  8. Garin B., Trap D., Gaumont R. Assessment of the EDTA seroagglutination test for the diagnosis of bovine brucellosis. Vet Rec. 1985 Oct 26;117(17):444–445. doi: 10.1136/vr.117.17.444. [DOI] [PubMed] [Google Scholar]
  9. Gaultney J. B., Wende R. D., Williams R. P. Microagglutination procedures for febrile agglutination tests. Appl Microbiol. 1971 Oct;22(4):635–640. doi: 10.1128/am.22.4.635-640.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Herr S., Roux D., Pieterson P. M. The reproducibility of results in bovine brucellosis serology and their correlation with the isolation of Brucella abortus. Onderstepoort J Vet Res. 1982 Jun;49(2):79–83. [PubMed] [Google Scholar]
  11. Herr S., te Brugge L. A., Guiney M. C. The value of the microtitre serum agglutination test as a second screening test in bovine brucellosis. Onderstepoort J Vet Res. 1982 Mar;49(1):23–28. [PubMed] [Google Scholar]
  12. Jiménez de Bagüs M. P., Marín C. M., Blasco J. M., Moriyón I., Gamazo C. An ELISA with Brucella lipopolysaccharide antigen for the diagnosis of B. melitensis infection in sheep and for the evaluation of serological responses following subcutaneous or conjunctival B. melitensis strain Rev 1 vaccination. Vet Microbiol. 1992 Feb;30(2-3):233–241. doi: 10.1016/0378-1135(92)90117-c. [DOI] [PubMed] [Google Scholar]
  13. Macmillan A. P., Cockrem D. S. Reduction of non-specific reactions to the Brucella abortus serum agglutination test by the addition of EDTA. Res Vet Sci. 1985 May;38(3):288–291. [PubMed] [Google Scholar]
  14. Massey E. D., Mangiafico J. A. Microagglutination test for detecting and measuring serum agglutinins of Francisella tularensis. Appl Microbiol. 1974 Jan;27(1):25–27. doi: 10.1128/am.27.1.25-27.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morgan W. J., MacKinnon D. J., Lawson J. R., Cullen G. A. The rose bengal plate agglutination test in the diagnosis of brucellosis. Vet Rec. 1969 Dec 6;85(23):636–641. doi: 10.1136/vr.85.23.636. [DOI] [PubMed] [Google Scholar]
  16. Morgan W. J., Richards R. A. The diagnosis, control and eradication of bovine brucellosis in Great Britain. Vet Rec. 1974 Jun 1;94(22):510–517. doi: 10.1136/vr.94.22.510. [DOI] [PubMed] [Google Scholar]
  17. Nielsen K., Stilwell K., Stemshorn B., Duncan R. Ethylenediaminetetraacetic acid (disodium salt)-labile bovine immunoglobulin M Fc binding to Brucella abortus: a cause of nonspecific agglutination. J Clin Microbiol. 1981 Jul;14(1):32–38. doi: 10.1128/jcm.14.1.32-38.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stemshorn B. W., Forbes L. B., Eaglesome M. D., Nielsen K. H., Robertson F. J., Samagh B. S. A comparison of standard serological tests for the diagnosis of bovine brucellosis in Canada. Can J Comp Med. 1985 Oct;49(4):391–394. [PMC free article] [PubMed] [Google Scholar]
  19. Stemshorn B. W. Recent progress in the diagnosis of brucellosis. Dev Biol Stand. 1984;56:325–340. [PubMed] [Google Scholar]
  20. Sutherland S. S. Evaluation of the enzyme-linked immunosorbent assay in the detection of cattle infected with Brucella abortus. Vet Microbiol. 1984 Dec;10(1):23–32. doi: 10.1016/0378-1135(84)90053-1. [DOI] [PubMed] [Google Scholar]
  21. Unel, Williams C. F., Stableforth A. W. Relative value of the agglutination test complement fixation test and Coombs (antigbulin) test in the detection of Brucella melitensis infection in sheep. J Comp Pathol. 1969 Apr;79(2):155–159. doi: 10.1016/0021-9975(69)90001-2. [DOI] [PubMed] [Google Scholar]
  22. Verger J. M., Garin-Bastuji B., Grayon M., Mahé A. M. La brucellose bovine à Brucella melitensis en France. Ann Rech Vet. 1989;20(1):93–102. [PubMed] [Google Scholar]
  23. Waghela S., Wandera J. G., Wagner G. G. Comparison of four serological tests in the diagnosis of caprine brucellosis. Res Vet Sci. 1980 Mar;28(2):168–171. [PubMed] [Google Scholar]
  24. Yong W. K., Edwards L. D. Evaluation of the microtitre spin agglutination assay in the diagnosis of bovine brucellosis. Vet Rec. 1986 Nov 1;119(18):454–455. doi: 10.1136/vr.119.18.454. [DOI] [PubMed] [Google Scholar]

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