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. 1995 Aug;33(8):2212–2215. doi: 10.1128/jcm.33.8.2212-2215.1995

Certain canine weakly beta-hemolytic intestinal spirochetes are phenotypically and genotypically related to spirochetes associated with human and porcine intestinal spirochetosis.

G E Duhamel 1, N Muniappa 1, M R Mathiesen 1, J L Johnson 1, J Toth 1, R O Elder 1, A R Doster 1
PMCID: PMC228371  PMID: 7559984

Abstract

Four canine weakly beta-hemolytic intestinal spirochetes associated with intestinal spirochetosis (IS-associated WBHIS) were compared with IS-associated human and porcine WBHIS and the type species for Serpulina hyodysenteriae and S. innocens by using phenotypic and genotypic parameters. The IS-associated canine, human, and porcine WBHIS belonged to a phyletic group distinct from but related to previously described Serpulina type species.

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Selected References

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  1. Elder R. O., Duhamel G. E., Schafer R. W., Mathiesen M. R., Ramanathan M. Rapid detection of Serpulina hyodysenteriae in diagnostic specimens by PCR. J Clin Microbiol. 1994 Jun;32(6):1497–1502. doi: 10.1128/jcm.32.6.1497-1502.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Harris D. L., Kinyon J. M., Mullin M. T., Glock R. D. Isolation and propagation of spirochetes from the colon of swine dysentery affected pigs. Can J Comp Med. 1972 Jan;36(1):74–76. [PMC free article] [PubMed] [Google Scholar]
  3. Hovind-Hougen K., Birch-Andersen A., Henrik-Nielsen R., Orholm M., Pedersen J. O., Teglbjaerg P. S., Thaysen E. H. Intestinal spirochetosis: morphological characterization and cultivation of the spirochete Brachyspira aalborgi gen. nov., sp. nov. J Clin Microbiol. 1982 Dec;16(6):1127–1136. doi: 10.1128/jcm.16.6.1127-1136.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jacques M., Girard C., Higgins R., Goyette G. Extensive colonization of the porcine colonic epithelium by a spirochete similar to Treponema innocens. J Clin Microbiol. 1989 May;27(5):1139–1141. doi: 10.1128/jcm.27.5.1139-1141.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jenkinson S. R., Wingar C. R. Selective medium for the isolation of Treponema hyodysenteriae. Vet Rec. 1981 Oct 24;109(17):384–385. doi: 10.1136/vr.109.17.384. [DOI] [PubMed] [Google Scholar]
  6. Joens L. A., Glock R. D., Kinyon J. M. Differentiation of Treponema hyodysenteriae from T innocens by enteropathogenicity testing in the CF1 mouse. Vet Rec. 1980 Dec 6;107(23):527–529. [PubMed] [Google Scholar]
  7. Jones M. J., Miller J. N., George W. L. Microbiological and biochemical characterization of spirochetes isolated from the feces of homosexual males. J Clin Microbiol. 1986 Dec;24(6):1071–1074. doi: 10.1128/jcm.24.6.1071-1074.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Koopman M. B., Käsbohrer A., Beckmann G., van der Zeijst B. A., Kusters J. G. Genetic similarity of intestinal spirochetes from humans and various animal species. J Clin Microbiol. 1993 Mar;31(3):711–716. doi: 10.1128/jcm.31.3.711-716.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kunkle R. A., Harris D. L., Kinyon J. M. Autoclaved liquid medium for propagation of Treponema hyodysenteriae. J Clin Microbiol. 1986 Oct;24(4):669–671. doi: 10.1128/jcm.24.4.669-671.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kunkle R. A., Kinyon J. M. Improved selective medium for the isolation of Treponema hyodysenteriae. J Clin Microbiol. 1988 Nov;26(11):2357–2360. doi: 10.1128/jcm.26.11.2357-2360.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Leach W. D., Lee A., Stubbs R. P. Localization of bacteria in the gastrointestinal tract: a possible explanation of intestinal spirochaetosis. Infect Immun. 1973 Jun;7(6):961–972. doi: 10.1128/iai.7.6.961-972.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lee J. I., Hampson D. J. Genetic characterisation of intestinal spirochaetes and their association with disease. J Med Microbiol. 1994 May;40(5):365–371. doi: 10.1099/00222615-40-5-365. [DOI] [PubMed] [Google Scholar]
  13. Lee J. I., Hampson D. J., Lymbery A. J., Harders S. J. The porcine intestinal spirochaetes: identification of new genetic groups. Vet Microbiol. 1993 Mar;34(3):273–285. doi: 10.1016/0378-1135(93)90017-2. [DOI] [PubMed] [Google Scholar]
  14. Lee J. I., McLaren A. J., Lymbery A. J., Hampson D. J. Human intestinal spirochetes are distinct from Serpulina hyodysenteriae. J Clin Microbiol. 1993 Jan;31(1):16–21. doi: 10.1128/jcm.31.1.16-21.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Milner J. A., Truelove K. G., Foster R. J., Sellwood R. Use of commercial enzyme kits and fatty acid production for the identification of Serpulina hyodysenteriae: a potential misdiagnosis. J Vet Diagn Invest. 1995 Jan;7(1):92–97. doi: 10.1177/104063879500700115. [DOI] [PubMed] [Google Scholar]
  16. Pindak F. F., Clapper W. E., Sherrod J. H. Incidence and distribution of spirochetes in the digestive tract of dogs. Am J Vet Res. 1965 Nov;26(115):1391–1402. [PubMed] [Google Scholar]
  17. Ralph D., McClelland M., Welsh J., Baranton G., Perolat P. Leptospira species categorized by arbitrarily primed polymerase chain reaction (PCR) and by mapped restriction polymorphisms in PCR-amplified rRNA genes. J Bacteriol. 1993 Feb;175(4):973–981. doi: 10.1128/jb.175.4.973-981.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ramanathan M., Duhamel G. E., Mathiesen M. R., Messier S. Identification and partial characterization of a group of weakly beta-hemolytic intestinal spirochetes of swine distinct from Serpulina innocens isolate B256. Vet Microbiol. 1993 Oct;37(1-2):53–64. doi: 10.1016/0378-1135(93)90182-7. [DOI] [PubMed] [Google Scholar]
  19. Songer J. G., Glock R. D., Schwartz K. J., Harris D. L. Isolation of Treponema hyodysenteriae from sources other than swine. J Am Vet Med Assoc. 1978 Feb 15;172(4):464–466. [PubMed] [Google Scholar]
  20. Stanton T. B. Proposal to change the genus designation Serpula to Serpulina gen. nov. containing the species Serpulina hyodysenteriae comb. nov. and Serpulina innocens comb. nov. Int J Syst Bacteriol. 1992 Jan;42(1):189–190. doi: 10.1099/00207713-42-1-189. [DOI] [PubMed] [Google Scholar]
  21. Takeuchi A., Jervis H. R., Nakazawa H., Robinson D. M. Spiral-shaped organisms on the surface colonic epithelium of the monkey and man. Am J Clin Nutr. 1974 Nov;27(11):1287–1296. doi: 10.1093/ajcn/27.11.1287. [DOI] [PubMed] [Google Scholar]
  22. Taylor D. J., Simmons J. R., Laird H. M. Production of diarrhoea and dysentery in pigs by feeding pure cultures of a spirochaete differing from Treponema hyodysenteriae. Vet Rec. 1980 Apr 12;106(15):326–332. doi: 10.1136/vr.106.15.326. [DOI] [PubMed] [Google Scholar]
  23. Turek J. J., Meyer R. C. Studies on a canine intestinal spirochete. I. Its isolation, cultivation and ultrastructure. Can J Comp Med. 1977 Jul;41(3):332–337. [PMC free article] [PubMed] [Google Scholar]
  24. Turek J. J., Meyer R. C. Studies on a canine intestinal spirochete: scanning electron microscopy of canine colonic mucosa. Infect Immun. 1978 Jun;20(3):853–855. doi: 10.1128/iai.20.3.853-855.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weber A., Schramm R. Untersuchungen zum Vorkommen von Treponemen in Kotproben von Hunden und Katzen mit und ohne Darmerkrankungen. Berl Munch Tierarztl Wochenschr. 1989 Mar 1;102(3):73–77. [PubMed] [Google Scholar]
  26. Welsh J., Pretzman C., Postic D., Saint Girons I., Baranton G., McClelland M. Genomic fingerprinting by arbitrarily primed polymerase chain reaction resolves Borrelia burgdorferi into three distinct phyletic groups. Int J Syst Bacteriol. 1992 Jul;42(3):370–377. doi: 10.1099/00207713-42-3-370. [DOI] [PubMed] [Google Scholar]
  27. Whipp S. C., Harris D. L., Kinyon J. M., Songer J. G., Glock R. D. Enteropathogenicity testing of Treponema hyodysenteriae in ligated colonic loops of swine. Am J Vet Res. 1978 Aug;39(8):1293–1296. [PubMed] [Google Scholar]

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