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. 1995 Aug;33(8):2224–2226. doi: 10.1128/jcm.33.8.2224-2226.1995

Genotypic analysis of pseudoepidemic due to contamination of Hanks' balanced salt solution with Candida parapsilosis.

S C Deresinski 1, K V Clemons 1, C A Kemper 1, K Roesch 1, B Walton 1, D A Stevens 1
PMCID: PMC228375  PMID: 7559988

Abstract

A cluster of isolates of Candida parapsilosis recovered from clinical specimens was demonstrated, by both classical and molecular epidemiological techniques, to have resulted from contamination in the laboratory. The source of the pseudoepidemic was a repeatedly utilized contaminated container of Hanks' balanced salt solution used in specimen processing. The patterns of restriction fragment length polymorphisms of DNA extracted from the clinical and environmental isolates were identical to each other but composed a newly identified unique C. parapsilosis DNA type.

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Selected References

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  1. Clemons K. V., Shankland G. S., Richardson M. D., Stevens D. A. Epidemiologic study by DNA typing of a Candida albicans outbreak in heroin addicts. J Clin Microbiol. 1991 Jan;29(1):205–207. doi: 10.1128/jcm.29.1.205-207.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hoffmann K. K., Weber D. J., Rutala W. A. Pseudoepidemic of Rhodotorula rubra in patients undergoing fiberoptic bronchoscopy. Infect Control Hosp Epidemiol. 1989 Nov;10(11):511–514. doi: 10.1086/645937. [DOI] [PubMed] [Google Scholar]
  3. Jarvis W. R., Martone W. J. Predominant pathogens in hospital infections. J Antimicrob Chemother. 1992 Apr;29 (Suppl A):19–24. doi: 10.1093/jac/29.suppl_a.19. [DOI] [PubMed] [Google Scholar]
  4. McGinley K. J., Larson E. L., Leyden J. J. Composition and density of microflora in the subungual space of the hand. J Clin Microbiol. 1988 May;26(5):950–953. doi: 10.1128/jcm.26.5.950-953.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mok W. Y., Barreto da Silva M. S. Mycoflora of the human dermal surfaces. Can J Microbiol. 1984 Oct;30(10):1205–1209. doi: 10.1139/m84-191. [DOI] [PubMed] [Google Scholar]
  6. O'Day D. M., Head W. S., Robinson R. D. An outbreak of Candida parapsilosis endophthalmitis: analysis of strains by enzyme profile and antifungal susceptibility. Br J Ophthalmol. 1987 Feb;71(2):126–129. doi: 10.1136/bjo.71.2.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. ROGERS A. L., BENEKE E. S. HUMAN PATHOGENIC FUNGI RECOVERED FROM BRASILIAN SOIL. Mycopathol Mycol Appl. 1964 Jan 31;22:15–20. doi: 10.1007/BF02058729. [DOI] [PubMed] [Google Scholar]
  8. Riddle D. L., Giger O., Miller L., Hall G. S., Woods G. L. Clinical comparison of the Baxter MicroScan Yeast Identification Panel and the Vitek Yeast Biochemical Card. Am J Clin Pathol. 1994 Apr;101(4):438–442. doi: 10.1093/ajcp/101.4.438. [DOI] [PubMed] [Google Scholar]
  9. Sanchez V., Vazquez J. A., Barth-Jones D., Dembry L., Sobel J. D., Zervos M. J. Nosocomial acquisition of Candida parapsilosis: an epidemiologic study. Am J Med. 1993 Jun;94(6):577–582. doi: 10.1016/0002-9343(93)90207-6. [DOI] [PubMed] [Google Scholar]
  10. Scherer S., Stevens D. A. Application of DNA typing methods to epidemiology and taxonomy of Candida species. J Clin Microbiol. 1987 Apr;25(4):675–679. doi: 10.1128/jcm.25.4.675-679.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schär G., Grehn M., von Graevenitz A. Persistent colonization of carbon dioxide incubators with Candida parapsilosis. Eur J Clin Microbiol Infect Dis. 1990 Oct;9(10):758–760. doi: 10.1007/BF02184689. [DOI] [PubMed] [Google Scholar]
  12. Solomon S. L., Alexander H., Eley J. W., Anderson R. L., Goodpasture H. C., Smart S., Furman R. M., Martone W. J. Nosocomial fungemia in neonates associated with intravascular pressure-monitoring devices. Pediatr Infect Dis. 1986 Nov-Dec;5(6):680–685. doi: 10.1097/00006454-198611000-00016. [DOI] [PubMed] [Google Scholar]
  13. Solomon S. L., Khabbaz R. F., Parker R. H., Anderson R. L., Geraghty M. A., Furman R. M., Martone W. J. An outbreak of Candida parapsilosis bloodstream infections in patients receiving parenteral nutrition. J Infect Dis. 1984 Jan;149(1):98–102. doi: 10.1093/infdis/149.1.98. [DOI] [PubMed] [Google Scholar]
  14. Stone J., Manasse R. Pseudoepidemic of urinary tract infections due to Trichosporon beigelii. Infect Control Hosp Epidemiol. 1989 Jul;10(7):312–315. doi: 10.1086/646034. [DOI] [PubMed] [Google Scholar]
  15. Valdes-Collazo L., Schultz A. J., Hazen T. C. Survival of Candida albicans in tropical marine and fresh waters. Appl Environ Microbiol. 1987 Aug;53(8):1762–1767. doi: 10.1128/aem.53.8.1762-1767.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Weems J. J., Jr Candida parapsilosis: epidemiology, pathogenicity, clinical manifestations, and antimicrobial susceptibility. Clin Infect Dis. 1992 Mar;14(3):756–766. doi: 10.1093/clinids/14.3.756. [DOI] [PubMed] [Google Scholar]

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