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. 1995 Sep;33(9):2415–2420. doi: 10.1128/jcm.33.9.2415-2420.1995

Outer surface protein C gene sequence analysis of Borrelia burgdorferi sensu lato isolates from Japan.

M Fukunaga 1, A Hamase 1
PMCID: PMC228426  PMID: 7494039

Abstract

The nucleotide sequences of the outer surface protein C gene (ospC) from Borrelia burgdorferi sensu lato isolates representing six different restriction fragment length polymorphism (RFLP) ribotype groups were determined, and the deduced amino acid sequences were aligned in comparison with the previously published OspC protein sequences. The sequence similarity analysis revealed the high sequence variability of OspC protein, and the degree of amino acid similarity ranged from 53.8 to 100% among 25 isolates. It has been reported that the representatives belonging to the three species of B. burgdorferi sensu lato showed a species-specific amino acid sequence motif at positions 23 to 35 (B. Wilske, S. Jauris-Heipke, R. Lobentanzer, I. Pradel, V. Preac-Mursic, D. Rössler, E. Soutschek, and R.C. Johnson, J. Clin. Microbiol. 33:103-109, 1995). Alignment with the OspC sequences of RFLP ribotype group IV, V, and VI isolates revealed that a sequence motif of all the isolates was quite similar to that of Borrelia garinii. A phylogenetic analysis based on OspC protein sequences also showed that most of the Japanese isolates were closely related to the species B. garinii. THe RFLP ribotype group IV species is predominant among clinical isolates of Lyme disease patients, reservoir rodents, and adult ticks in Japan. Although the isolates differed from type strains of the three delineated genospecies in genetic and immunological characteristics, it is likely that the spirochetes diverged within the species level.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Adam T., Gassmann G. S., Rasiah C., Göbel U. B. Phenotypic and genotypic analysis of Borrelia burgdorferi isolates from various sources. Infect Immun. 1991 Aug;59(8):2579–2585. doi: 10.1128/iai.59.8.2579-2585.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson J. F. Epizootiology of Borrelia in Ixodes tick vectors and reservoir hosts. Rev Infect Dis. 1989 Sep-Oct;11 (Suppl 6):S1451–S1459. doi: 10.1093/clinids/11.supplement_6.s1451. [DOI] [PubMed] [Google Scholar]
  3. Baranton G., Postic D., Saint Girons I., Boerlin P., Piffaretti J. C., Assous M., Grimont P. A. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. Int J Syst Bacteriol. 1992 Jul;42(3):378–383. doi: 10.1099/00207713-42-3-378. [DOI] [PubMed] [Google Scholar]
  4. Canica M. M., Nato F., du Merle L., Mazie J. C., Baranton G., Postic D. Monoclonal antibodies for identification of Borrelia afzelii sp. nov. associated with late cutaneous manifestations of Lyme borreliosis. Scand J Infect Dis. 1993;25(4):441–448. doi: 10.3109/00365549309008525. [DOI] [PubMed] [Google Scholar]
  5. Fuchs R., Jauris S., Lottspeich F., Preac-Mursic V., Wilske B., Soutschek E. Molecular analysis and expression of a Borrelia burgdorferi gene encoding a 22 kDa protein (pC) in Escherichia coli. Mol Microbiol. 1992 Feb;6(4):503–509. doi: 10.1111/j.1365-2958.1992.tb01495.x. [DOI] [PubMed] [Google Scholar]
  6. Fukunaga M., Mifuchi I. Unique organization of Leptospira interrogans rRNA genes. J Bacteriol. 1989 Nov;171(11):5763–5767. doi: 10.1128/jb.171.11.5763-5767.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fukunaga M., Sohnaka M., Nakao M., Miyamoto K. Evaluation of genetic divergence of borrelial isolates from Lyme disease patients in Hokkaido, Japan, by rRNA gene probes. J Clin Microbiol. 1993 Aug;31(8):2044–2048. doi: 10.1128/jcm.31.8.2044-2048.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fukunaga M., Sohnaka M., Takahashi Y., Nakao M., Miyamoto K. Antigenic and genetic characterization of Borrelia species isolated from Ixodes persulcatus in Hokkaido, Japan. J Clin Microbiol. 1993 May;31(5):1388–1391. doi: 10.1128/jcm.31.5.1388-1391.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fukunaga M., Sohnaka M., Yanagihara Y. Analysis of Borrelia species associated with Lyme disease by rRNA gene restriction fragment length polymorphism. J Gen Microbiol. 1993 Jun;139(Pt 6):1141–1146. doi: 10.1099/00221287-139-6-1141. [DOI] [PubMed] [Google Scholar]
  10. Fukunaga M., Takahashi Y. Pulsed field gel electrophoresis analysis of Borrelia burgdorferi sensu lato isolated in Japan and taxonomic implications with Lyme disease spirochetes. Microbiol Immunol. 1994;38(9):747–751. doi: 10.1111/j.1348-0421.1994.tb01851.x. [DOI] [PubMed] [Google Scholar]
  11. Fukunaga M., Yanagihara Y., Sohnaka M. The 23S/5S ribosomal RNA genes (rrl/rrf) are separate from the 16S ribosomal RNA gene (rrs) in Borrelia burgdorferi, the aetiological agent of Lyme disease. J Gen Microbiol. 1992 May;138(5):871–877. doi: 10.1099/00221287-138-5-871. [DOI] [PubMed] [Google Scholar]
  12. Higgins D. G., Bleasby A. J., Fuchs R. CLUSTAL V: improved software for multiple sequence alignment. Comput Appl Biosci. 1992 Apr;8(2):189–191. doi: 10.1093/bioinformatics/8.2.189. [DOI] [PubMed] [Google Scholar]
  13. Jaenson T. G. The epidemiology of lyme borreliosis. Parasitol Today. 1991 Feb;7(2):39–45. doi: 10.1016/0169-4758(91)90187-s. [DOI] [PubMed] [Google Scholar]
  14. Jauris-Heipke S., Fuchs R., Motz M., Preac-Mursic V., Schwab E., Soutschek E., Will G., Wilske B. Genetic heterogenity of the genes coding for the outer surface protein C (OspC) and the flagellin of Borrelia burgdorferi. Med Microbiol Immunol. 1993 Mar;182(1):37–50. doi: 10.1007/BF00195949. [DOI] [PubMed] [Google Scholar]
  15. Kubo N., Arashima Y., Yoshida M., Kawabata M., Nishinarita S., Hayama T., Sawada S., Horie T., Nakao M., Miyamoto K. Questionnaire surveys of cases of tick bite and Lyme borreliosis in hunters in Hokkaido with reference to detection of anti-Borrelia burgdorferi antibody. Intern Med. 1992 Oct;31(10):1163–1168. doi: 10.2169/internalmedicine.31.1163. [DOI] [PubMed] [Google Scholar]
  16. Lane R. S., Piesman J., Burgdorfer W. Lyme borreliosis: relation of its causative agent to its vectors and hosts in North America and Europe. Annu Rev Entomol. 1991;36:587–609. doi: 10.1146/annurev.en.36.010191.003103. [DOI] [PubMed] [Google Scholar]
  17. Marconi R. T., Garon C. F. Development of polymerase chain reaction primer sets for diagnosis of Lyme disease and for species-specific identification of Lyme disease isolates by 16S rRNA signature nucleotide analysis. J Clin Microbiol. 1992 Nov;30(11):2830–2834. doi: 10.1128/jcm.30.11.2830-2834.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Marconi R. T., Samuels D. S., Garon C. F. Transcriptional analyses and mapping of the ospC gene in Lyme disease spirochetes. J Bacteriol. 1993 Feb;175(4):926–932. doi: 10.1128/jb.175.4.926-932.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Marconi R. T., Samuels D. S., Schwan T. G., Garon C. F. Identification of a protein in several Borrelia species which is related to OspC of the Lyme disease spirochetes. J Clin Microbiol. 1993 Oct;31(10):2577–2583. doi: 10.1128/jcm.31.10.2577-2583.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Matuschka F. R., Fischer P., Heiler M., Richter D., Spielman A. Capacity of European animals as reservoir hosts for the Lyme disease spirochete. J Infect Dis. 1992 Mar;165(3):479–483. doi: 10.1093/infdis/165.3.479. [DOI] [PubMed] [Google Scholar]
  21. Miyamoto K., Nakao M., Sato N., Mori M. Isolation of Lyme disease spirochetes from an ixodid tick in Hokkaido, Japan. Acta Trop. 1991 Apr;49(1):65–68. doi: 10.1016/0001-706x(91)90031-e. [DOI] [PubMed] [Google Scholar]
  22. Nakao M., Miyamoto K., Fukunaga M., Hashimoto Y., Takahashi H. Comparative studies on Borrelia afzelii isolated from a patient of Lyme disease, Ixodes persulcatus ticks, and Apodemus speciosus rodents in Japan. Microbiol Immunol. 1994;38(6):413–420. doi: 10.1111/j.1348-0421.1994.tb01801.x. [DOI] [PubMed] [Google Scholar]
  23. Nakao M., Miyamoto K., Fukunaga M. Lyme disease spirochetes in Japan: enzootic transmission cycles in birds, rodents, and Ixodes persulcatus ticks. J Infect Dis. 1994 Oct;170(4):878–882. doi: 10.1093/infdis/170.4.878. [DOI] [PubMed] [Google Scholar]
  24. Nakao M., Miyamoto K., Kawagishi N., Hashimoto Y., Iizuka H. Comparison of Borrelia burgdorferi isolated from humans and ixodid ticks in Hokkaido, Japan. Microbiol Immunol. 1992;36(11):1189–1193. doi: 10.1111/j.1348-0421.1992.tb02121.x. [DOI] [PubMed] [Google Scholar]
  25. Nakao M., Miyamoto K., Uchikawa K., Fujita H. Characterization of Borrelia burgdorferi isolated from Ixodes persulcatus and Ixodes ovatus ticks in Japan. Am J Trop Med Hyg. 1992 Oct;47(4):505–511. doi: 10.4269/ajtmh.1992.47.505. [DOI] [PubMed] [Google Scholar]
  26. Park K. H., Chang W. H., Schwan T. G. Identification and characterization of Lyme disease spirochetes, Borrelia burgdorferi sensu lato, isolated in Korea. J Clin Microbiol. 1993 Jul;31(7):1831–1837. doi: 10.1128/jcm.31.7.1831-1837.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Postic D., Assous M. V., Grimont P. A., Baranton G. Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. Int J Syst Bacteriol. 1994 Oct;44(4):743–752. doi: 10.1099/00207713-44-4-743. [DOI] [PubMed] [Google Scholar]
  28. Sadziene A., Wilske B., Ferdows M. S., Barbour A. G. The cryptic ospC gene of Borrelia burgdorferi B31 is located on a circular plasmid. Infect Immun. 1993 May;61(5):2192–2195. doi: 10.1128/iai.61.5.2192-2195.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Theisen M., Frederiksen B., Lebech A. M., Vuust J., Hansen K. Polymorphism in ospC gene of Borrelia burgdorferi and immunoreactivity of OspC protein: implications for taxonomy and for use of OspC protein as a diagnostic antigen. J Clin Microbiol. 1993 Oct;31(10):2570–2576. doi: 10.1128/jcm.31.10.2570-2576.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wallich R., Helmes C., Schaible U. E., Lobet Y., Moter S. E., Kramer M. D., Simon M. M. Evaluation of genetic divergence among Borrelia burgdorferi isolates by use of OspA, fla, HSP60, and HSP70 gene probes. Infect Immun. 1992 Nov;60(11):4856–4866. doi: 10.1128/iai.60.11.4856-4866.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wilske B., Jauris-Heipke S., Lobentanzer R., Pradel I., Preac-Mursic V., Rössler D., Soutschek E., Johnson R. C. Phenotypic analysis of outer surface protein C (OspC) of Borrelia burgdorferi sensu lato by monoclonal antibodies: relationship to genospecies and OspA serotype. J Clin Microbiol. 1995 Jan;33(1):103–109. doi: 10.1128/jcm.33.1.103-109.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wilske B., Preac-Mursic V., Göbel U. B., Graf B., Jauris S., Soutschek E., Schwab E., Zumstein G. An OspA serotyping system for Borrelia burgdorferi based on reactivity with monoclonal antibodies and OspA sequence analysis. J Clin Microbiol. 1993 Feb;31(2):340–350. doi: 10.1128/jcm.31.2.340-350.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wilske B., Preac-Mursic V., Jauris S., Hofmann A., Pradel I., Soutschek E., Schwab E., Will G., Wanner G. Immunological and molecular polymorphisms of OspC, an immunodominant major outer surface protein of Borrelia burgdorferi. Infect Immun. 1993 May;61(5):2182–2191. doi: 10.1128/iai.61.5.2182-2191.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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