Abstract
Recovery rates from Middlebrook 7H11-selective 7H11 biplates and Lowenstein-Jensen slants (LJ) used with BACTEC 12B vials were compared for 5,399 specimens. For 578 specimens that were inoculated onto three media, 580 mycobacteria were isolated, including 277 (48%) Mycobacterium avium complex isolates, 230 (40%) Mycobacterium tuberculosis isolates, and 73 (12%) other mycobacteria. For BACTEC 12B vials, 506 (87%) cultures were positive, 45 (8%) were negative, and 29 (5%) were lost to contamination; for 7H11-7H11-selective biplates, 469 (81%) cultures were positive, 95 (16%) were negative, and 16 (3%) were lost to contamination; for LJ, 230 (40%) cultures were positive, 111 (19%) were negative, and 239 (41%) were lost to contamination. For routine cultures, use of plate media is superior to use of LJ.
Full Text
The Full Text of this article is available as a PDF (189.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anargyros P., Astill D. S., Lim I. S. Comparison of improved BACTEC and Lowenstein-Jensen media for culture of mycobacteria from clinical specimens. J Clin Microbiol. 1990 Jun;28(6):1288–1291. doi: 10.1128/jcm.28.6.1288-1291.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Isenberg H. D., D'Amato R. F., Heifets L., Murray P. R., Scardamaglia M., Jacobs M. C., Alperstein P., Niles A. Collaborative feasibility study of a biphasic system (Roche Septi-Chek AFB) for rapid detection and isolation of mycobacteria. J Clin Microbiol. 1991 Aug;29(8):1719–1722. doi: 10.1128/jcm.29.8.1719-1722.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morgan M. A., Horstmeier C. D., DeYoung D. R., Roberts G. D. Comparison of a radiometric method (BACTEC) and conventional culture media for recovery of mycobacteria from smear-negative specimens. J Clin Microbiol. 1983 Aug;18(2):384–388. doi: 10.1128/jcm.18.2.384-388.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Park C. H., Hixon D. L., Ferguson C. B., Hall S. L., Risheim C. C., Cook C. B. Rapid recovery of mycobacteria from clinical specimens using automated radiometric technic. Am J Clin Pathol. 1984 Mar;81(3):341–345. doi: 10.1093/ajcp/81.3.341. [DOI] [PubMed] [Google Scholar]
- Roberts G. D., Goodman N. L., Heifets L., Larsh H. W., Lindner T. H., McClatchy J. K., McGinnis M. R., Siddiqi S. H., Wright P. Evaluation of the BACTEC radiometric method for recovery of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis from acid-fast smear-positive specimens. J Clin Microbiol. 1983 Sep;18(3):689–696. doi: 10.1128/jcm.18.3.689-696.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stager C. E., Libonati J. P., Siddiqi S. H., Davis J. R., Hooper N. M., Baker J. F., Carter M. E. Role of solid media when used in conjunction with the BACTEC system for mycobacterial isolation and identification. J Clin Microbiol. 1991 Jan;29(1):154–157. doi: 10.1128/jcm.29.1.154-157.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woods G. L. Disease due to the Mycobacterium avium complex in patients infected with human immunodeficiency virus: diagnosis and susceptibility testing. Clin Infect Dis. 1994 Apr;18 (Suppl 3):S227–S232. doi: 10.1093/clinids/18.supplement_3.s227. [DOI] [PubMed] [Google Scholar]