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. 1995 Oct;33(10):2562–2566. doi: 10.1128/jcm.33.10.2562-2566.1995

Comparative study of five methods for intratypic differentiation of polioviruses.

H G van der Avoort 1, B P Hull 1, T Hovi 1, M A Pallansch 1, O M Kew 1, R Crainic 1, D J Wood 1, M N Mulders 1, A M van Loon 1
PMCID: PMC228529  PMID: 8567883

Abstract

A coded panel of 90 poliovirus isolates, 30 of each of the three known serotypes, was used to evaluate five methods for the intratypic differentiation of polioviruses: (i) an enzyme-linked immunosorbent assay with polyclonal cross-absorbed antisera (PAb-E), (ii) a neutralization assay with type-specific monoclonal antibodies (MAb-N), (iii) a restriction fragment length polymorphism (RFLP) assay, (iv) a Sabin vaccine strain-specific PCR assay, and (v) a Sabin vaccine strain-specific cRNA probe hybridization (ProHyb) assay. Sequence analysis was used for the definitive characterization of the strains. The panel was distributed to five laboratories; each laboratory analyzed the strains by at least two methods. Each method was used by three or four laboratories. The total performance scores (percentage correct results per number of tests) of the five methods were 96.7% for PAb-E, 93.9% for MAb-N, 91.9% for RFLP assay, 93.3% for Sabin vaccine strain-specific PCR, and 97.4% for Sabin vaccine strain-specific ProHyb. Consistent results were obtained by each laboratory for 88 of 90 isolates (97.8%) examined by PAb-E, 81 of 90 isolates (90.0%) examined by MAb-N, 78 of 90 isolates (86.7%) examined by RFLP assay, 81 of 90 isolates (90.0%) examined by PCR, and 89 of 90 isolates (98.9%) examined by ProHyb assay. Six strains were classified differently by different methods. It is recommended that at least two methods be used for the intratypic differentiation of poliovirus isolates, and each method should be based on a different principle (i.e., antigenic properties and nucleotide sequence composition).(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Balanant J., Guillot S., Candrea A., Delpeyroux F., Crainic R. The natural genomic variability of poliovirus analyzed by a restriction fragment length polymorphism assay. Virology. 1991 Oct;184(2):645–654. doi: 10.1016/0042-6822(91)90434-d. [DOI] [PubMed] [Google Scholar]
  2. Crainic R., Couillin P., Blondel B., Cabau N., Boué A., Horodniceanu F. Natural variation of poliovirus neutralization epitopes. Infect Immun. 1983 Sep;41(3):1217–1225. doi: 10.1128/iai.41.3.1217-1225.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. De L., Nottay B., Yang C. F., Holloway B. P., Pallansch M., Kew O. Identification of vaccine-related polioviruses by hybridization with specific RNA probes. J Clin Microbiol. 1995 Mar;33(3):562–571. doi: 10.1128/jcm.33.3.562-571.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Deshpande J. M., Dave K. H. Intratypic characterization of poliovirus type 1 isolates. Indian J Med Res. 1991 Jul;93:202–207. [PubMed] [Google Scholar]
  5. Ferguson M., Magrath D. I., Minor P. D., Schild G. C. WHO collaborative study on the use of monoclonal antibodies for the intratypic differentiation of poliovirus strains. Bull World Health Organ. 1986;64(2):239–246. [PMC free article] [PubMed] [Google Scholar]
  6. Glikmann G., Moynihan M., Petersen I., Vestergaard B. F. Intratypic differentiation of poliovirus strains by enzyme-linked immunosorbent assay (ELISA): poliovirus type 1. Dev Biol Stand. 1983;55:199–208. [PubMed] [Google Scholar]
  7. Hovi T., Cantell K., Huovilainen A., Kinnunen E., Kuronen T., Lapinleimu K., Pöyry T., Roivainen M., Salama N., Stenvik M. Outbreak of paralytic poliomyelitis in Finland: widespread circulation of antigenically altered poliovirus type 3 in a vaccinated population. Lancet. 1986 Jun 21;1(8495):1427–1432. doi: 10.1016/s0140-6736(86)91566-7. [DOI] [PubMed] [Google Scholar]
  8. Jarzabek Z., Zabicka J., John A., Howlett J., Dunn G., Wood D. J. Application of monoclonal antibody panels in the virological and epidemiological review of poliomyelitis in Poland, 1981-1990. Bull World Health Organ. 1992;70(3):327–333. [PMC free article] [PubMed] [Google Scholar]
  9. Mulders M. N., Lipskaya G. Y., van der Avoort H. G., Koopmans M. P., Kew O. M., van Loon A. M. Molecular epidemiology of wild poliovirus type 1 in Europe, the Middle East, and the Indian subcontinent. J Infect Dis. 1995 Jun;171(6):1399–1405. doi: 10.1093/infdis/171.6.1399. [DOI] [PubMed] [Google Scholar]
  10. Nkowane B. M., Wassilak S. G., Orenstein W. A., Bart K. J., Schonberger L. B., Hinman A. R., Kew O. M. Vaccine-associated paralytic poliomyelitis. United States: 1973 through 1984. JAMA. 1987 Mar 13;257(10):1335–1340. [PubMed] [Google Scholar]
  11. Osterhaus A. D., van Wezel A. L., Hazendonk T. G., UytdeHaag F. G., van Asten J. A., van Steenis B. Monoclonal antibodies to polioviruses. Comparison of intratypic strain differentiation of poliovirus type 1 using monoclonal antibodies versus cross-absorbed antisera. Intervirology. 1983;20(2-3):129–136. doi: 10.1159/000149381. [DOI] [PubMed] [Google Scholar]
  12. Pöyry T., Kinnunen L., Kapsenberg J., Kew O., Hovi T. Type 3 poliovirus/Finland/1984 is genetically related to common Mediterranean strains. J Gen Virol. 1990 Nov;71(Pt 11):2535–2541. doi: 10.1099/0022-1317-71-11-2535. [DOI] [PubMed] [Google Scholar]
  13. Schweiger B., Schreier E., Böthig B., López-Pila J. M. Differentiation of vaccine and wild-type polioviruses using polymerase chain reaction and restriction enzyme analysis. Arch Virol. 1994;134(1-2):39–50. doi: 10.1007/BF01379105. [DOI] [PubMed] [Google Scholar]
  14. Takeda N., Sakae K., Agboatwalla M., Isomura S., Hondo R., Inouye S. Differentiation between wild and vaccine-derived strains of poliovirus by stringent microplate hybridization of PCR products. J Clin Microbiol. 1994 Jan;32(1):202–204. doi: 10.1128/jcm.32.1.202-204.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tambini G., Andrus J. K., Marques E., Boshell J., Pallansch M., de Quadros C. A., Kew O. Direct detection of wild poliovirus circulation by stool surveys of healthy children and analysis of community wastewater. J Infect Dis. 1993 Dec;168(6):1510–1514. doi: 10.1093/infdis/168.6.1510. [DOI] [PubMed] [Google Scholar]
  16. Ward N. A., Milstien J. B., Hull H. F., Hull B. P., Kim-Farley R. J. The WHO-EPI initiative for the global eradication of poliomyelitis. Biologicals. 1993 Dec;21(4):327–333. doi: 10.1006/biol.1993.1092. [DOI] [PubMed] [Google Scholar]
  17. Wright P. F., Kim-Farley R. J., de Quadros C. A., Robertson S. E., Scott R. M., Ward N. A., Henderson R. H. Strategies for the global eradication of poliomyelitis by the year 2000. N Engl J Med. 1991 Dec 19;325(25):1774–1779. doi: 10.1056/NEJM199112193252504. [DOI] [PubMed] [Google Scholar]
  18. Yang C. F., De L., Holloway B. P., Pallansch M. A., Kew O. M. Detection and identification of vaccine-related polioviruses by the polymerase chain reaction. Virus Res. 1991 Jul;20(2):159–179. doi: 10.1016/0168-1702(91)90107-7. [DOI] [PubMed] [Google Scholar]
  19. Yang C. F., De L., Yang S. J., Ruiz Gómez J., Cruz J. R., Holloway B. P., Pallansch M. A., Kew O. M. Genotype-specific in vitro amplification of sequences of the wild type 3 polioviruses from Mexico and Guatemala. Virus Res. 1992 Aug;24(3):277–296. doi: 10.1016/0168-1702(92)90124-r. [DOI] [PubMed] [Google Scholar]
  20. da Silva E. E., Pallansch M. A., Holloway B. P., Oliveira M. J., Schatzmayr H. G., Kew O. M. Oligonucleotide probes for the specific detection of the wild poliovirus types 1 and 3 endemic to Brazil. Intervirology. 1991;32(3):149–159. doi: 10.1159/000150195. [DOI] [PubMed] [Google Scholar]
  21. van Wezel A. L., Hazendonk A. G. Intratypic serodifferentiation of poliomyelitis virus strains by strain-specific antisera. Intervirology. 1979;11(1):2–8. doi: 10.1159/000149005. [DOI] [PubMed] [Google Scholar]
  22. van der Avoort H. G., Reimerink J. H., Ras A., Mulders M. N., van Loon A. M. Isolation of epidemic poliovirus from sewage during the 1992-3 type 3 outbreak in The Netherlands. Epidemiol Infect. 1995 Jun;114(3):481–491. doi: 10.1017/s0950268800052195. [DOI] [PMC free article] [PubMed] [Google Scholar]

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