Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1995 Oct;33(10):2607–2611. doi: 10.1128/jcm.33.10.2607-2611.1995

Human cytomegalovirus (CMV) DNA in plasma reflects quantity of CMV DNA present in leukocytes.

D Zipeto 1, S Morris 1, C Hong 1, A Dowling 1, R Wolitz 1, T C Merigan 1, L Rasmussen 1
PMCID: PMC228538  PMID: 8567891

Abstract

A quantitative DNA amplification assay for human cytomegalovirus (CMV) DNA has been used to evaluate the relationship between quantities of CMV DNA in plasma and those in infected leukocytes (WBC) from human immunodeficiency virus-infected patients. The target sequence for DNA amplification was a region of the immediate-early 1 gene of CMV. The quantitation assay uses an internal control that is coamplified with each patient sample DNA and contains a sequence for detection by colorimetric hybridization with the same bases, but in different order than in the CMV immediate-early 1 region used for hybridization of amplified patient sample DNA. Results showed that patients with CMV disease had more CMV DNA in both WBC and plasma than those without disease. However, in this study, copy numbers of CMV DNA in WBC were higher than those in plasma. The gB and gH variants were the same in plasma and WBC.

Full Text

The Full Text of this article is available as a PDF (196.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brytting M., Xu W., Wahren B., Sundqvist V. A. Cytomegalovirus DNA detection in sera from patients with active cytomegalovirus infections. J Clin Microbiol. 1992 Aug;30(8):1937–1941. doi: 10.1128/jcm.30.8.1937-1941.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chou S. W., Dennison K. M. Analysis of interstrain variation in cytomegalovirus glycoprotein B sequences encoding neutralization-related epitopes. J Infect Dis. 1991 Jun;163(6):1229–1234. doi: 10.1093/infdis/163.6.1229. [DOI] [PubMed] [Google Scholar]
  3. Chou S. W. Differentiation of cytomegalovirus strains by restriction analysis of DNA sequences amplified from clinical specimens. J Infect Dis. 1990 Sep;162(3):738–742. doi: 10.1093/infdis/162.3.738. [DOI] [PubMed] [Google Scholar]
  4. Chou S. Comparative analysis of sequence variation in gp116 and gp55 components of glycoprotein B of human cytomegalovirus. Virology. 1992 May;188(1):388–390. doi: 10.1016/0042-6822(92)90771-g. [DOI] [PubMed] [Google Scholar]
  5. Chou S. Effect of interstrain variation on diagnostic DNA amplification of the cytomegalovirus major immediate-early gene region. J Clin Microbiol. 1992 Sep;30(9):2307–2310. doi: 10.1128/jcm.30.9.2307-2310.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chou S. Molecular epidemiology of envelope glycoprotein H of human cytomegalovirus. J Infect Dis. 1992 Sep;166(3):604–607. doi: 10.1093/infdis/166.3.604. [DOI] [PubMed] [Google Scholar]
  7. Diviacco S., Norio P., Zentilin L., Menzo S., Clementi M., Biamonti G., Riva S., Falaschi A., Giacca M. A novel procedure for quantitative polymerase chain reaction by coamplification of competitive templates. Gene. 1992 Dec 15;122(2):313–320. doi: 10.1016/0378-1119(92)90220-j. [DOI] [PubMed] [Google Scholar]
  8. Einhorn L., Ost A. Cytomegalovirus infection of human blood cells. J Infect Dis. 1984 Feb;149(2):207–214. doi: 10.1093/infdis/149.2.207. [DOI] [PubMed] [Google Scholar]
  9. Freymuth F., Gennetay E., Petitjean J., Eugene G., Hurault de Ligny B., Ryckelynck J. P., Legoff C., Hazera P., Bazin C. Comparison of nested PCR for detection of DNA in plasma with pp65 leukocytic antigenemia procedure for diagnosis of human cytomegalovirus infection. J Clin Microbiol. 1994 Jun;32(6):1614–1618. doi: 10.1128/jcm.32.6.1614-1618.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gerna G., Baldanti F., Sarasini A., Furione M., Percivalle E., Revello M. G., Zipeto D., Zella D. Effect of foscarnet induction treatment on quantitation of human cytomegalovirus (HCMV) DNA in peripheral blood polymorphonuclear leukocytes and aqueous humor of AIDS patients with HCMV retinitis. The Italian Foscarnet Study Group. Antimicrob Agents Chemother. 1994 Jan;38(1):38–44. doi: 10.1128/aac.38.1.38. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gerna G., Furione M., Baldanti F., Sarasini A. Comparative quantitation of human cytomegalovirus DNA in blood leukocytes and plasma of transplant and AIDS patients. J Clin Microbiol. 1994 Nov;32(11):2709–2717. doi: 10.1128/jcm.32.11.2709-2717.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gerna G., Parea M., Percivalle E., Zipeto D., Silini E., Barbarini G., Milanesi G. Human cytomegalovirus viraemia in HIV-1-seropositive patients at various clinical stages of infection. AIDS. 1990 Oct;4(10):1027–1031. doi: 10.1097/00002030-199010000-00014. [DOI] [PubMed] [Google Scholar]
  13. Hansen K. K., Ricksten A., Hofmann B., Norrild B., Olofsson S., Mathiesen L. Detection of cytomegalovirus DNA in serum correlates with clinical cytomegalovirus retinitis in AIDS. J Infect Dis. 1994 Nov;170(5):1271–1274. doi: 10.1093/infdis/170.5.1271. [DOI] [PubMed] [Google Scholar]
  14. Ishigaki S., Takeda M., Kura T., Ban N., Saitoh T., Sakamaki S., Watanabe N., Kohgo Y., Niitsu Y. Cytomegalovirus DNA in the sera of patients with cytomegalovirus pneumonia. Br J Haematol. 1991 Oct;79(2):198–204. doi: 10.1111/j.1365-2141.1991.tb04522.x. [DOI] [PubMed] [Google Scholar]
  15. Miller M. J., Bovey S., Pado K., Bruckner D. A., Wagar E. A. Application of PCR to multiple specimen types for diagnosis of cytomegalovirus infection: comparison with cell culture and shell vial assay. J Clin Microbiol. 1994 Jan;32(1):5–10. doi: 10.1128/jcm.32.1.5-10.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Patel R., Smith T. F., Espy M., Wiesner R. H., Krom R. A., Portela D., Paya C. V. Detection of cytomegalovirus DNA in sera of liver transplant recipients. J Clin Microbiol. 1994 Jun;32(6):1431–1434. doi: 10.1128/jcm.32.6.1431-1434.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rasmussen L., Morris S., Hamed K., Merigan T. C. Human cytomegalovirus DNA is present in CD45+ cells in semen from human immunodeficiency virus-infected patients. J Infect Dis. 1995 Feb;171(2):432–436. doi: 10.1093/infdis/171.2.432. [DOI] [PubMed] [Google Scholar]
  18. Rasmussen L., Morris S., Zipeto D., Fessel J., Wolitz R., Dowling A., Merigan T. C. Quantitation of human cytomegalovirus DNA from peripheral blood cells of human immunodeficiency virus-infected patients could predict cytomegalovirus retinitis. J Infect Dis. 1995 Jan;171(1):177–182. doi: 10.1093/infdis/171.1.177. [DOI] [PubMed] [Google Scholar]
  19. Shibata D., Martin W. J., Appleman M. D., Causey D. M., Leedom J. M., Arnheim N. Detection of cytomegalovirus DNA in peripheral blood of patients infected with human immunodeficiency virus. J Infect Dis. 1988 Dec;158(6):1185–1192. doi: 10.1093/infdis/158.6.1185. [DOI] [PubMed] [Google Scholar]
  20. Spector S. A., Merrill R., Wolf D., Dankner W. M. Detection of human cytomegalovirus in plasma of AIDS patients during acute visceral disease by DNA amplification. J Clin Microbiol. 1992 Sep;30(9):2359–2365. doi: 10.1128/jcm.30.9.2359-2365.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Storch G. A., Buller R. S., Bailey T. C., Ettinger N. A., Langlois T., Gaudreault-Keener M., Welby P. L. Comparison of PCR and pp65 antigenemia assay with quantitative shell vial culture for detection of cytomegalovirus in blood leukocytes from solid-organ transplant recipients. J Clin Microbiol. 1994 Apr;32(4):997–1003. doi: 10.1128/jcm.32.4.997-1003.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zipeto D., Baldanti F., Zella D., Furione M., Cavicchini A., Milanesi G., Gerna G. Quantification of human cytomegalovirus DNA in peripheral blood polymorphonuclear leukocytes of immunocompromised patients by the polymerase chain reaction. J Virol Methods. 1993 Sep;44(1):45–55. doi: 10.1016/0166-0934(93)90006-d. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES