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. 1995 Oct;33(10):2631–2636. doi: 10.1128/jcm.33.10.2631-2636.1995

Intermethod variation in detection of human papillomavirus DNA in cervical smears.

H L Smits 1, L J Bollen 1, S P Tjong-A-Hung 1, J Vonk 1, J Van Der Velden 1, F J Ten Kate 1, J A Kaan 1, B W Mol 1, J Ter Schegget 1
PMCID: PMC228545  PMID: 8567896

Abstract

In order to investigate the reliability of detection of human papillomavirus (HPV) DNA in cervical smears, we have compared the performance of two HPV PCR systems, the CPI/IIG and MY09/11 primer-mediated PCRs and the Hybrid Capture System HPV DNA detection test (hybrid capture assay), in detecting HPV DNA in cervical smears. We also included in our study the MY09/11B PCR plus SHARP (solution hybridization assay for PCR products) Signal System. This SHARP Signal System was recently developed to detect MY09/11B-generated biotinylated PCR products. The detection rate of the hybrid capture assay was lower than those of the CPI/IIG and MY09/11 PCRs and the MY09/11B PCR plus SHARP Signal System. The detection rates of the CPI/IIG PCR and the MY09/11B PCR plus SHARP Signal System were similar and higher than that of the conventional MY09/11 PCR system. The agreement beyond chance of the PCR methods was nearly perfect (kappa value between 0.82 and 0.84). The agreement beyond chance of the hybrid capture assay and the PCR methods was fair to good (kappa value between 0.64 and 0.70). The systems detected HPV DNA in different but overlapping sets of smears. Our results indicate that each of the detection methods alone underestimates the prevalence of HPV.

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Selected References

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  1. Arends M. J., Donaldson Y. K., Duvall E., Wyllie A. H., Bird C. C. HPV in full thickness cervical biopsies: high prevalence in CIN 2 and CIN 3 detected by a sensitive PCR method. J Pathol. 1991 Dec;165(4):301–309. doi: 10.1002/path.1711650405. [DOI] [PubMed] [Google Scholar]
  2. Bauer H. M., Ting Y., Greer C. E., Chambers J. C., Tashiro C. J., Chimera J., Reingold A., Manos M. M. Genital human papillomavirus infection in female university students as determined by a PCR-based method. JAMA. 1991 Jan 23;265(4):472–477. [PubMed] [Google Scholar]
  3. Bergeron C., Barrasso R., Beaudenon S., Flamant P., Croissant O., Orth G. Human papillomaviruses associated with cervical intraepithelial neoplasia. Great diversity and distinct distribution in low- and high-grade lesions. Am J Surg Pathol. 1992 Jul;16(7):641–649. doi: 10.1097/00000478-199207000-00002. [DOI] [PubMed] [Google Scholar]
  4. Boom R., Sol C. J., Salimans M. M., Jansen C. L., Wertheim-van Dillen P. M., van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 1990 Mar;28(3):495–503. doi: 10.1128/jcm.28.3.495-503.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cornelissen M. T., Bots T., Briët M. A., Jebbink M. F., Struyk A. P., van den Tweel J. G., Greer C. E., Smits H. L., ter Schegget J. Detection of human papillomavirus types by the polymerase chain reaction and the differentiation between high-risk and low-risk cervical lesions. Virchows Arch B Cell Pathol Incl Mol Pathol. 1992;62(3):167–171. doi: 10.1007/BF02899679. [DOI] [PubMed] [Google Scholar]
  6. Cuzick J., Terry G., Ho L., Hollingworth T., Anderson M. Human papillomavirus type 16 in cervical smears as predictor of high-grade cervical intraepithelial neoplasia [corrected]. Lancet. 1992 Apr 18;339(8799):959–960. doi: 10.1016/0140-6736(92)91532-d. [DOI] [PubMed] [Google Scholar]
  7. Franco E. L. The sexually transmitted disease model for cervical cancer: incoherent epidemiologic findings and the role of misclassification of human papillomavirus infection. Epidemiology. 1991 Mar;2(2):98–106. [PubMed] [Google Scholar]
  8. Fujinaga Y., Shimada M., Okazawa K., Fukushima M., Kato I., Fujinaga K. Simultaneous detection and typing of genital human papillomavirus DNA using the polymerase chain reaction. J Gen Virol. 1991 May;72(Pt 5):1039–1044. doi: 10.1099/0022-1317-72-5-1039. [DOI] [PubMed] [Google Scholar]
  9. Kiyabu M. T., Shibata D., Arnheim N., Martin W. J., Fitzgibbons P. L. Detection of human papillomavirus in formalin-fixed, invasive squamous carcinomas using the polymerase chain reaction. Am J Surg Pathol. 1989 Mar;13(3):221–224. doi: 10.1097/00000478-198903000-00007. [DOI] [PubMed] [Google Scholar]
  10. Koutsky L. A., Holmes K. K., Critchlow C. W., Stevens C. E., Paavonen J., Beckmann A. M., DeRouen T. A., Galloway D. A., Vernon D., Kiviat N. B. A cohort study of the risk of cervical intraepithelial neoplasia grade 2 or 3 in relation to papillomavirus infection. N Engl J Med. 1992 Oct 29;327(18):1272–1278. doi: 10.1056/NEJM199210293271804. [DOI] [PubMed] [Google Scholar]
  11. Lungu O., Sun X. W., Felix J., Richart R. M., Silverstein S., Wright T. C., Jr Relationship of human papillomavirus type to grade of cervical intraepithelial neoplasia. JAMA. 1992 May 13;267(18):2493–2496. [PubMed] [Google Scholar]
  12. Melkert P. W., Hopman E., van den Brule A. J., Risse E. K., van Diest P. J., Bleker O. P., Helmerhorst T., Schipper M. E., Meijer C. J., Walboomers J. M. Prevalence of HPV in cytomorphologically normal cervical smears, as determined by the polymerase chain reaction, is age-dependent. Int J Cancer. 1993 Apr 1;53(6):919–923. doi: 10.1002/ijc.2910530609. [DOI] [PubMed] [Google Scholar]
  13. Resnick R. M., Cornelissen M. T., Wright D. K., Eichinger G. H., Fox H. S., ter Schegget J., Manos M. M. Detection and typing of human papillomavirus in archival cervical cancer specimens by DNA amplification with consensus primers. J Natl Cancer Inst. 1990 Sep 19;82(18):1477–1484. doi: 10.1093/jnci/82.18.1477. [DOI] [PubMed] [Google Scholar]
  14. Smits H. L., Tieben L. M., Tjong-A-Hung S. P., Jebbink M. F., Minnaar R. P., Jansen C. L., ter Schegget J. Detection and typing of human papillomaviruses present in fixed and stained archival cervical smears by a consensus polymerase chain reaction and direct sequence analysis allow the identification of a broad spectrum of human papillomavirus types. J Gen Virol. 1992 Dec;73(Pt 12):3263–3268. doi: 10.1099/0022-1317-73-12-3263. [DOI] [PubMed] [Google Scholar]
  15. Tieben L. M., ter Schegget J., Minnaar R. P., Bouwes Bavinck J. N., Berkhout R. J., Vermeer B. J., Jebbink M. F., Smits H. L. Detection of cutaneous and genital HPV types in clinical samples by PCR using consensus primers. J Virol Methods. 1993 May;42(2-3):265–279. doi: 10.1016/0166-0934(93)90038-s. [DOI] [PubMed] [Google Scholar]
  16. Van Den Brule A. J., Walboomers J. M., Du Maine M., Kenemans P., Meijer C. J. Difference in prevalence of human papillomavirus genotypes in cytomorphologically normal cervical smears is associated with a history of cervical intraepithelial neoplasia. Int J Cancer. 1991 May 30;48(3):404–408. doi: 10.1002/ijc.2910480317. [DOI] [PubMed] [Google Scholar]
  17. Yoshikawa H., Kawana T., Kitagawa K., Mizuno M., Yoshikura H., Iwamoto A. Amplification and typing of multiple cervical cancer-associated human papillomavirus DNAs using a single pair of primers. Int J Cancer. 1990 May 15;45(5):990–992. doi: 10.1002/ijc.2910450537. [DOI] [PubMed] [Google Scholar]
  18. van den Brule A. J., Snijders P. J., Gordijn R. L., Bleker O. P., Meijer C. J., Walboomers J. M. General primer-mediated polymerase chain reaction permits the detection of sequenced and still unsequenced human papillomavirus genotypes in cervical scrapes and carcinomas. Int J Cancer. 1990 Apr 15;45(4):644–649. doi: 10.1002/ijc.2910450412. [DOI] [PubMed] [Google Scholar]

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