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. 1995 Oct;33(10):2653–2659. doi: 10.1128/jcm.33.10.2653-2659.1995

An Indian strain of hepatitis E virus (HEV): cloning, sequence, and expression of structural region and antibody responses in sera from individuals from an area of high-level HEV endemicity.

S K Panda 1, S K Nanda 1, M Zafrullah 1, I H Ansari 1, M H Ozdener 1, S Jameel 1
PMCID: PMC228549  PMID: 8567900

Abstract

Hepatitis E virus (HEV) is responsible for a majority of sporadic and epidemic viral hepatitides in India and other developing countries. Even though the genomes of four geographically distinct strains of HEV have been cloned and sequenced, the Indian strain of HEV remains largely uncharacterized. We have cloned and sequenced about 2.2 kb of the HEV genome constituting the structural region from an Indian strain of HEV. The nucleotide and amino acid sequences show a high degree of conservation with sequences from other HEV strains. Open reading frames (ORF) 2 and 3 were expressed in Escherichia coli as N-terminal hexahistidine epitope fusions. The purified proteins were then used in an immunoblot assay to evaluate the antibody status in sera from individuals from an area of high-level HEV endemicity. The anti-ORF2 antibodies were found to be nonspecific and could not be correlated to clinical disease. The immunoglobulin M anti-ORF3 was found to be specific for the presence of acute disease. The implications of these findings in HEV diagnosis and vaccine development are discussed.

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Selected References

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  1. Aye T. T., Uchida T., Ma X. Z., Iida F., Shikata T., Zhuang H., Win K. M. Complete nucleotide sequence of a hepatitis E virus isolated from the Xinjiang epidemic (1986-1988) of China. Nucleic Acids Res. 1992 Jul 11;20(13):3512–3512. doi: 10.1093/nar/20.13.3512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradley D. W. Enterically-transmitted non-A, non-B hepatitis. Br Med Bull. 1990 Apr;46(2):442–461. doi: 10.1093/oxfordjournals.bmb.a072409. [DOI] [PubMed] [Google Scholar]
  3. Carl M., Isaacs S. N., Kaur M., He J., Tam A. W., Yarbough P. O., Reyes G. R. Expression of hepatitis E virus putative structural proteins in recombinant vaccinia viruses. Clin Diagn Lab Immunol. 1994 Mar;1(2):253–256. doi: 10.1128/cdli.1.2.253-256.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dawson G. J., Chau K. H., Cabal C. M., Yarbough P. O., Reyes G. R., Mushahwar I. K. Solid-phase enzyme-linked immunosorbent assay for hepatitis E virus IgG and IgM antibodies utilizing recombinant antigens and synthetic peptides. J Virol Methods. 1992 Jul;38(1):175–186. doi: 10.1016/0166-0934(92)90180-l. [DOI] [PubMed] [Google Scholar]
  5. He J., Tam A. W., Yarbough P. O., Reyes G. R., Carl M. Expression and diagnostic utility of hepatitis E virus putative structural proteins expressed in insect cells. J Clin Microbiol. 1993 Aug;31(8):2167–2173. doi: 10.1128/jcm.31.8.2167-2173.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Huang C. C., Nguyen D., Fernandez J., Yun K. Y., Fry K. E., Bradley D. W., Tam A. W., Reyes G. R. Molecular cloning and sequencing of the Mexico isolate of hepatitis E virus (HEV). Virology. 1992 Dec;191(2):550–558. doi: 10.1016/0042-6822(92)90230-m. [DOI] [PubMed] [Google Scholar]
  7. Jameel S., Durgapal H., Habibullah C. M., Khuroo M. S., Panda S. K. Enteric non-A, non-B hepatitis: epidemics, animal transmission, and hepatitis E virus detection by the polymerase chain reaction. J Med Virol. 1992 Aug;37(4):263–270. doi: 10.1002/jmv.1890370405. [DOI] [PubMed] [Google Scholar]
  8. Kaur M., Hyams K. C., Purdy M. A., Krawczynski K., Ching W. M., Fry K. E., Reyes G. R., Bradley D. W., Carl M. Human linear B-cell epitopes encoded by the hepatitis E virus include determinants in the RNA-dependent RNA polymerase. Proc Natl Acad Sci U S A. 1992 May 1;89(9):3855–3858. doi: 10.1073/pnas.89.9.3855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Khudyakov YuE, Favorov M. O., Jue D. L., Hine T. K., Fields H. A. Immunodominant antigenic regions in a structural protein of the hepatitis E virus. Virology. 1994 Jan;198(1):390–393. doi: 10.1006/viro.1994.1048. [DOI] [PubMed] [Google Scholar]
  10. Khudyakov Y. E., Khudyakova N. S., Fields H. A., Jue D., Starling C., Favorov M. O., Krawczynski K., Polish L., Mast E., Margolis H. Epitope mapping in proteins of hepatitis E virus. Virology. 1993 May;194(1):89–96. doi: 10.1006/viro.1993.1238. [DOI] [PubMed] [Google Scholar]
  11. Khuroo M. S., Duermeyer W., Zargar S. A., Ahanger M. A., Shah M. A. Acute sporadic non-A, non-B hepatitis in India. Am J Epidemiol. 1983 Sep;118(3):360–364. doi: 10.1093/oxfordjournals.aje.a113643. [DOI] [PubMed] [Google Scholar]
  12. Khuroo M. S. Study of an epidemic of non-A, non-B hepatitis. Possibility of another human hepatitis virus distinct from post-transfusion non-A, non-B type. Am J Med. 1980 Jun;68(6):818–824. doi: 10.1016/0002-9343(80)90200-4. [DOI] [PubMed] [Google Scholar]
  13. Khuroo M. S., Teli M. R., Skidmore S., Sofi M. A., Khuroo M. I. Incidence and severity of viral hepatitis in pregnancy. Am J Med. 1981 Feb;70(2):252–255. doi: 10.1016/0002-9343(81)90758-0. [DOI] [PubMed] [Google Scholar]
  14. Li F., Zhuang H., Kolivas S., Locarnini S. A., Anderson D. A. Persistent and transient antibody responses to hepatitis E virus detected by western immunoblot using open reading frame 2 and 3 and glutathione S-transferase fusion proteins. J Clin Microbiol. 1994 Sep;32(9):2060–2066. doi: 10.1128/jcm.32.9.2060-2066.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nanda S. K., Yalcinkaya K., Panigrahi A. K., Acharya S. K., Jameel S., Panda S. K. Etiological role of hepatitis E virus in sporadic fulminant hepatitis. J Med Virol. 1994 Feb;42(2):133–137. doi: 10.1002/jmv.1890420207. [DOI] [PubMed] [Google Scholar]
  16. Purdy M. A., McCaustland K. A., Krawczynski K., Tam A., Beach M. J., Tassopoulos N. C., Reyes G. R., Bradley D. W. Expression of a hepatitis E virus (HEV)-trpE fusion protein containing epitopes recognized by antibodies in sera from human cases and experimentally infected primates. Arch Virol. 1992;123(3-4):335–349. doi: 10.1007/BF01317268. [DOI] [PubMed] [Google Scholar]
  17. Ray R., Jameel S., Manivel V., Ray R. Indian hepatitis E virus shows a major deletion in the small open reading frame. Virology. 1992 Jul;189(1):359–362. doi: 10.1016/0042-6822(92)90716-3. [DOI] [PubMed] [Google Scholar]
  18. Reyes G. R., Purdy M. A., Kim J. P., Luk K. C., Young L. M., Fry K. E., Bradley D. W. Isolation of a cDNA from the virus responsible for enterically transmitted non-A, non-B hepatitis. Science. 1990 Mar 16;247(4948):1335–1339. doi: 10.1126/science.2107574. [DOI] [PubMed] [Google Scholar]
  19. Tam A. W., Smith M. M., Guerra M. E., Huang C. C., Bradley D. W., Fry K. E., Reyes G. R. Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome. Virology. 1991 Nov;185(1):120–131. doi: 10.1016/0042-6822(91)90760-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tsarev S. A., Emerson S. U., Reyes G. R., Tsareva T. S., Legters L. J., Malik I. A., Iqbal M., Purcell R. H. Characterization of a prototype strain of hepatitis E virus. Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):559–563. doi: 10.1073/pnas.89.2.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. VISWANATHAN R. Epidemiology. Indian J Med Res. 1957 Jan;45(Suppl):1–29. [PubMed] [Google Scholar]
  22. Wong D. C., Purcell R. H., Sreenivasan M. A., Prasad S. R., Pavri K. M. Epidemic and endemic hepatitis in India: evidence for a non-A, non-B hepatitis virus aetiology. Lancet. 1980 Oct 25;2(8200):876–879. doi: 10.1016/s0140-6736(80)92045-0. [DOI] [PubMed] [Google Scholar]
  23. Yarbough P. O., Tam A. W., Fry K. E., Krawczynski K., McCaustland K. A., Bradley D. W., Reyes G. R. Hepatitis E virus: identification of type-common epitopes. J Virol. 1991 Nov;65(11):5790–5797. doi: 10.1128/jvi.65.11.5790-5797.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

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