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. 1995 Oct;33(10):2798–2800. doi: 10.1128/jcm.33.10.2798-2800.1995

Recovery of Helicobacter pylori from gastric biopsy specimens is not dependent on the transport medium used.

R Roosendaal 1, E J Kuipers 1, A S Peña 1, J de Graaff 1
PMCID: PMC228582  PMID: 8567932

Abstract

The survival of six clinical isolates of Helicobacter pylori at room temperature was investigated after suspension in five different media: brucella broth with 5% lysed horse blood (BBLH), phosphate-buffered saline (PBS), 20% glucose, Stuart medium, and PBS with 10% Fildes enrichment (PBS-F). Only in BBLH and PBS-F no decrease in mean bacterial numbers was observed during the 24-h study period. No H. pylori isolates could be cultured from Stuart medium after 7 h of incubation. In contrast, the recovery rates in PBS-F or Stuart medium of H. pylori isolates from gastric tissue specimens collected from 19 H. pylori-positive patients were not significantly different even after a delay of culture of up to 24 h. Our data show that the medium composition is not critical for the survival of H. pylori within gastric tissue specimens.

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Selected References

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  1. Coudron P. E., Kirby D. F. Comparison of rapid urease tests, staining techniques, and growth on different solid media for detection of Campylobacter pylori. J Clin Microbiol. 1989 Jul;27(7):1527–1530. doi: 10.1128/jcm.27.7.1527-1530.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Goodwin C. S., Blincow E. D., Warren J. R., Waters T. E., Sanderson C. R., Easton L. Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastric mucosa. J Clin Pathol. 1985 Oct;38(10):1127–1131. doi: 10.1136/jcp.38.10.1127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hartmann D., von Graevenitz A. A note on name, viability and urease tests of Campylobacter pylori. Eur J Clin Microbiol. 1987 Feb;6(1):82–83. doi: 10.1007/BF02097207. [DOI] [PubMed] [Google Scholar]
  4. Karim Q. N., Maxwell R. H. Survival of Campylobacter pylori in artificially contaminated milk. J Clin Pathol. 1989 Jul;42(7):778–778. doi: 10.1136/jcp.42.7.778-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kjøller M., Fischer A., Justesen T. Transport conditions and number of biopsies necessary for culture of Helicobacter pylori. Eur J Clin Microbiol Infect Dis. 1991 Mar;10(3):166–167. doi: 10.1007/BF01964450. [DOI] [PubMed] [Google Scholar]
  6. Owen R. J., On S. L., Costas M. Potential transport medium for Campylobacter pylori. J Clin Pathol. 1988 Dec;41(12):1337–1338. doi: 10.1136/jcp.41.12.1337-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Parsonnet J., Friedman G. D., Vandersteen D. P., Chang Y., Vogelman J. H., Orentreich N., Sibley R. K. Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med. 1991 Oct 17;325(16):1127–1131. doi: 10.1056/NEJM199110173251603. [DOI] [PubMed] [Google Scholar]
  8. Petit M. D., Van der Velden U., Van Winkelhoff A. J., De Graaff J. Preserving the motility of microorganisms. Oral Microbiol Immunol. 1991 Apr;6(2):107–110. doi: 10.1111/j.1399-302x.1991.tb00460.x. [DOI] [PubMed] [Google Scholar]
  9. Queiroz D. M., Mendes E. N., Rocha G. A. Indicator medium for isolation of Campylobacter pylori. J Clin Microbiol. 1987 Dec;25(12):2378–2379. doi: 10.1128/jcm.25.12.2378-2379.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Roosendaal R., Kuipers E. J., van den Brule A. J., Peña A. S., Uyterlinde A. M., Walboomers J. M., Meuwissen S. G., de Graaff J. Importance of the fiberoptic endoscope cleaning procedure for detection of Helicobacter pylori in gastric biopsy specimens by PCR. J Clin Microbiol. 1994 Apr;32(4):1123–1126. doi: 10.1128/jcm.32.4.1123-1126.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. STUART R. D., TOSHACH S. R., PATSULA T. M. The problem of transport of specimens for culture of Gonococci. Can J Public Health. 1954 Feb;45(2):73–83. [PubMed] [Google Scholar]
  12. Soltesz V., Zeeberg B., Wadström T. Optimal survival of Helicobacter pylori under various transport conditions. J Clin Microbiol. 1992 Jun;30(6):1453–1456. doi: 10.1128/jcm.30.6.1453-1456.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Veenendaal R. A., Lichtendahl-Bernards A. T., Peña A. S., Endtz H. P., van Boven C. P., Lamers C. B. Effect of transport medium and transportation time on culture of Helicobacter pylori from gastric biopsy specimens. J Clin Pathol. 1993 Jun;46(6):561–563. doi: 10.1136/jcp.46.6.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Xia H. X., Keane C. T., O'Morain C. A. Determination of the optimal transport system for Helicobacter pylori cultures. J Med Microbiol. 1993 Nov;39(5):334–337. doi: 10.1099/00222615-39-5-334. [DOI] [PubMed] [Google Scholar]

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