Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1995 Nov;33(11):2859–2863. doi: 10.1128/jcm.33.11.2859-2863.1995

Detection of newly recognized rodent parvoviruses by PCR.

D G Besselsen 1, C L Besch-Williford 1, D J Pintel 1, C L Franklin 1, R R Hook Jr 1, L K Riley 1
PMCID: PMC228595  PMID: 8576334

Abstract

Several autonomous parvovirus isolates distinct from the prototypic rodent parvoviruses have recently been identified. These include variants of a mouse orphan parvovirus (MOPV) and a hamster isolate designated hamster orphan parvovirus (HOPV). In this study, a PCR primer set specific for these newly identified rodent parvoviruses was designed on the basis of DNA sequence comparisons of these isolates with other autonomous parvoviruses. The specificity of the primer set was determined by testing viral preparations of seven different parvoviruses and eight other viruses known to infect rodents. The PCR assay amplified the expected 260-bp product only in the presence of DNA from MOPV, HOPV, or LuIII a parvovirus of unknown species origin. The assay was able to detect as little as 10 pg of MOPV viral DNA or 1 pg of HOPV viral DNA, and it was able to detect MOPV in tissues from naturally infected mice and HOPV in tissues from experimentally infected hamsters. In contrast, the 260-bp product was not amplified from tissues of MOPV-negative mice or mock-infected hamsters. Our findings indicate that this PCR assay provides a rapid, specific, and sensitive method for the detection of MOPV in mice, HOPV in hamsters, and MOPV and HOPV in cell culture systems and that it may also be useful for the detection of LuIII contamination of cell culture systems.

Full Text

The Full Text of this article is available as a PDF (222.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ball-Goodrich L. J., Johnson E. Molecular characterization of a newly recognized mouse parvovirus. J Virol. 1994 Oct;68(10):6476–6486. doi: 10.1128/jvi.68.10.6476-6486.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bass L. R., Hetrick F. M. Persistent infection of a human lymphocyte cell line (Molt-4) with the kilham rat virus. J Infect Dis. 1978 Feb;137(2):210–212. doi: 10.1093/infdis/137.2.210. [DOI] [PubMed] [Google Scholar]
  3. Bonnard G. D., Manders E. K., Campbell D. A., Jr, Herberman R. B., Collins M. J., Jr Immunosuppressive activity of a subline of the mouse EL-4 lymphoma. Evidence for minute virus of mice causing the inhibition. J Exp Med. 1976 Jan 1;143(1):187–205. doi: 10.1084/jem.143.1.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Campbell D. A., Jr, Manders E. K., Oehler J. R., Bonnard G. D., Oldham R. K., Herberman R. B. Inhibition of in vitro lymphoproliferative responses by in vivo passaged rat 13762 mammary adenocarcinoma cells. I. Characteristics of inhibition and evidence for an infectious agent. Cell Immunol. 1977 Oct;33(2):364–377. doi: 10.1016/0008-8749(77)90165-4. [DOI] [PubMed] [Google Scholar]
  5. Campbell D. A., Jr, Staal S. P., Manders E. K., Bonnard G. D., Oldham R. K., Salzman L. A., Herberman R. B. Inhibition of in vitro lymphoproliferative responses by in vivo passaged rat 13762 mammary adenocarcinoma cells. II. Evidenceth Kilham rat virus is responsible for the inhibitory effect. Cell Immunol. 1977 Oct;33(2):378–391. doi: 10.1016/0008-8749(77)90166-6. [DOI] [PubMed] [Google Scholar]
  6. Collins M. J., Jr, Parker J. C. Murine virus contaminants of leukemia viruses and transplantable tumors. J Natl Cancer Inst. 1972 Oct;49(4):1139–1143. [PubMed] [Google Scholar]
  7. Crawford L. V. A minute virus of mice. Virology. 1966 Aug;29(4):605–612. doi: 10.1016/0042-6822(66)90284-4. [DOI] [PubMed] [Google Scholar]
  8. Darrigrand A. A., Singh S. B., Lang C. M. Effects of Kilham rat virus on natural killer cell-mediated cytotoxicity in brown Norway and Wistar Furth rats. Am J Vet Res. 1984 Jan;45(1):200–202. [PubMed] [Google Scholar]
  9. Faisst S., Bartnitzke S., Schlehofer J. R., zur Hausen H. Persistence of parvovirus H-1 DNA in human B- and T-lymphoma cells. Virus Res. 1990 Jun;16(2):211–223. doi: 10.1016/0168-1702(90)90024-6. [DOI] [PubMed] [Google Scholar]
  10. Gravitt P. E., Manos M. M. Nucleic acid hybridization methods to detect microorganisms. Lab Anim Sci. 1993 Feb;43(1):5–10. [PubMed] [Google Scholar]
  11. Hallauer C., Kronauer G., Siegl G. Parvoiruses as contaminants of permanent human cell lines. I. Virus isolation from 1960-1970. Arch Gesamte Virusforsch. 1971;35(1):80–90. doi: 10.1007/BF01249755. [DOI] [PubMed] [Google Scholar]
  12. Jacoby R. O., Johnson E. A., Ball-Goodrich L., Smith A. L., McKisic M. D. Characterization of mouse parvovirus infection by in situ hybridization. J Virol. 1995 Jun;69(6):3915–3919. doi: 10.1128/jvi.69.6.3915-3919.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KILHAM L., OLIVIER L. J. A latent virus of rats isolated in tissue culture. Virology. 1959 Apr;7(4):428–437. doi: 10.1016/0042-6822(59)90071-6. [DOI] [PubMed] [Google Scholar]
  14. McKisic M. D., Lancki D. W., Otto G., Padrid P., Snook S., Cronin D. C., 2nd, Lohmar P. D., Wong T., Fitch F. W. Identification and propagation of a putative immunosuppressive orphan parvovirus in cloned T cells. J Immunol. 1993 Jan 15;150(2):419–428. [PubMed] [Google Scholar]
  15. Nicklas W., Kraft V., Meyer B. Contamination of transplantable tumors, cell lines, and monoclonal antibodies with rodent viruses. Lab Anim Sci. 1993 Aug;43(4):296–300. [PubMed] [Google Scholar]
  16. Ron D., Tal J. Coevolution of cells and virus as a mechanism for the persistence of lymphotropic minute virus of mice in L-cells. J Virol. 1985 Aug;55(2):424–430. doi: 10.1128/jvi.55.2.424-430.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SHEIN H. M., ENDERS J. F. Multiplication and cytopathogenicity of Simian vacuolating virus 40 in cultures of human tissues. Proc Soc Exp Biol Med. 1962 Mar;109:495–500. doi: 10.3181/00379727-109-27246. [DOI] [PubMed] [Google Scholar]
  18. Smith A. L., Jacoby R. O., Johnson E. A., Paturzo F., Bhatt P. N. In vivo studies with an "orphan" parvovirus of mice. Lab Anim Sci. 1993 Apr;43(2):175–182. [PubMed] [Google Scholar]
  19. Tattersall P., Bratton J. Reciprocal productive and restrictive virus-cell interactions of immunosuppressive and prototype strains of minute virus of mice. J Virol. 1983 Jun;46(3):944–955. doi: 10.1128/jvi.46.3.944-955.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Toolan H. W., Dalldore G., Barclay M., Chandra S., Moore A. E. AN UNIDENTIFIED, FILTRABLE AGENT ISOLATED FROM TRANSPLANTED HUMAN TUMORS. Proc Natl Acad Sci U S A. 1960 Sep;46(9):1256–1258. doi: 10.1073/pnas.46.9.1256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. de Souza M., Smith A. L. Comparison of isolation in cell culture with conventional and modified mouse antibody production tests for detection of murine viruses. J Clin Microbiol. 1989 Jan;27(1):185–187. doi: 10.1128/jcm.27.1.185-187.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES