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. 1995 Nov;33(11):2940–2947. doi: 10.1128/jcm.33.11.2940-2947.1995

Genotypic and phenotypic characterization of Helicobacter cinaedi and Helicobacter fennelliae strains isolated from humans and animals.

J A Kiehlbauch 1, D J Brenner 1, D N Cameron 1, A G Steigerwalt 1, J M Makowski 1, C N Baker 1, C M Patton 1, I K Wachsmuth 1
PMCID: PMC228611  PMID: 8576350

Abstract

By DNA-DNA hybridization, we classified 26 human strains, 4 dog and cat strains, and 4 hamster strains putatively identified as Helicobacter cinaedi as well as 2 human strains and 2 animal strains of Helicobacter fennelliae. All but one human strain belonged to the same hybridization group as the type strain of H. cinaedi. The animal strains also appeared to belong to this hybridization group. Both human strains of H. fennelliae were shown to be H. fennelliae by DNA-DNA hybridization, but both animal strains were less than 15% related to the type strain. All strains were also characterized by plasmid profiles and ribotyping. Plasmids were found in 23% of the human strains, 100% of the hamster strains, and 33% of the dog and cat strains. Human strains were essentially identical by ribotyping, but were clearly differentiated from the hamster and dog and cat strains. Some strains may be difficult to culture on primary isolation; we found that our strains grew well on anaerobic CDC agar, brucella agar, and tryptic soy agar II. Our H. cinaedi and H. fennelliae strains differed from those previously described because some were resistant to cephalothin: some H. cinaedi strains were also resistant to nalidixic acid. All isolates were also characterized by antimicrobial susceptibility testing. We found that human strains of H. cinaedi were more resistant to clindamycin and erythromycin than were animal isolates; 19% of the human strains were resistant to ciprofloxacin. Therefore, we recommend that antimicrobial susceptibility results be obtained before initiating therapy for H. cinaedi and H. fennelliae infections.

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Selected References

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  1. Brenner D. J., McWhorter A. C., Knutson J. K., Steigerwalt A. G. Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol. 1982 Jun;15(6):1133–1140. doi: 10.1128/jcm.15.6.1133-1140.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burnens A. P., Nicolet J. Three supplementary diagnostic tests for Campylobacter species and related organisms. J Clin Microbiol. 1993 Mar;31(3):708–710. doi: 10.1128/jcm.31.3.708-710.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burnens A. P., Stanley J., Schaad U. B., Nicolet J. Novel Campylobacter-like organism resembling Helicobacter fennelliae isolated from a boy with gastroenteritis and from dogs. J Clin Microbiol. 1993 Jul;31(7):1916–1917. doi: 10.1128/jcm.31.7.1916-1917.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Decker C. F., Martin G. J., Barham W. B., Paparello S. F. Bacteremia due to Campylobacter cinaedi in a patient infected with the human immunodeficiency virus. Clin Infect Dis. 1992 Jul;15(1):178–179. doi: 10.1093/clinids/15.1.178-a. [DOI] [PubMed] [Google Scholar]
  5. Fennell C. L., Totten P. A., Quinn T. C., Patton D. L., Holmes K. K., Stamm W. E. Characterization of Campylobacter-like organisms isolated from homosexual men. J Infect Dis. 1984 Jan;149(1):58–66. doi: 10.1093/infdis/149.1.58. [DOI] [PubMed] [Google Scholar]
  6. Flores B. M., Fennell C. L., Holmes K. K., Stamm W. E. In vitro susceptibilities of Campylobacter-like organisms to twenty antimicrobial agents. Antimicrob Agents Chemother. 1985 Aug;28(2):188–191. doi: 10.1128/aac.28.2.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gebhart C. J., Fennell C. L., Murtaugh M. P., Stamm W. E. Campylobacter cinaedi is normal intestinal flora in hamsters. J Clin Microbiol. 1989 Jul;27(7):1692–1694. doi: 10.1128/jcm.27.7.1692-1694.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grayson M. L., Tee W., Dwyer B. Gastroenteritis associated with Campylobacter cinaedi. Med J Aust. 1989 Feb 20;150(4):214–215. doi: 10.5694/j.1326-5377.1989.tb136430.x. [DOI] [PubMed] [Google Scholar]
  9. Kiehlbauch J. A., Brenner D. J., Nicholson M. A., Baker C. N., Patton C. M., Steigerwalt A. G., Wachsmuth I. K. Campylobacter butzleri sp. nov. isolated from humans and animals with diarrheal illness. J Clin Microbiol. 1991 Feb;29(2):376–385. doi: 10.1128/jcm.29.2.376-385.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kiehlbauch J. A., Cameron D. N., Wachsmuth I. K. Evaluation of ribotyping techniques as applied to Arcobacter, Campylobacter and Helicobacter. Mol Cell Probes. 1994 Apr;8(2):109–115. doi: 10.1006/mcpr.1994.1015. [DOI] [PubMed] [Google Scholar]
  11. Kiehlbauch J. A., Plikaytis B. D., Swaminathan B., Cameron D. N., Wachsmuth I. K. Restriction fragment length polymorphisms in the ribosomal genes for species identification and subtyping of aerotolerant Campylobacter species. J Clin Microbiol. 1991 Aug;29(8):1670–1676. doi: 10.1128/jcm.29.8.1670-1676.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kiehlbauch J. A., Tauxe R. V., Baker C. N., Wachsmuth I. K. Helicobacter cinaedi-associated bacteremia and cellulitis in immunocompromised patients. Ann Intern Med. 1994 Jul 15;121(2):90–93. doi: 10.7326/0003-4819-121-2-199407150-00002. [DOI] [PubMed] [Google Scholar]
  13. Lambert M. A., Patton C. M., Barrett T. J., Moss C. W. Differentiation of Campylobacter and Campylobacter-like organisms by cellular fatty acid composition. J Clin Microbiol. 1987 Apr;25(4):706–713. doi: 10.1128/jcm.25.4.706-713.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Patton C. M., Mitchell S. W., Potter M. E., Kaufmann A. F. Comparison of selective media for primary isolation of Campylobacter fetus subsp. jejuni. J Clin Microbiol. 1981 Feb;13(2):326–330. doi: 10.1128/jcm.13.2.326-330.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Popovic-Uroic T., Patton C. M., Nicholson M. A., Kiehlbauch J. A. Evaluation of the indoxyl acetate hydrolysis test for rapid differentiation of Campylobacter, Helicobacter, and Wolinella species. J Clin Microbiol. 1990 Oct;28(10):2335–2339. doi: 10.1128/jcm.28.10.2335-2339.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Portnoy D. A., Moseley S. L., Falkow S. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun. 1981 Feb;31(2):775–782. doi: 10.1128/iai.31.2.775-782.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Quinn T. C., Goodell S. E., Fennell C., Wang S. P., Schuffler M. D., Holmes K. K., Stamm W. E. Infections with Campylobacter jejuni and Campylobacter-like organisms in homosexual men. Ann Intern Med. 1984 Aug;101(2):187–192. doi: 10.7326/0003-4819-101-2-187. [DOI] [PubMed] [Google Scholar]
  18. Sacks L. V., Labriola A. M., Gill V. J., Gordin F. M. Use of ciprofloxacin for successful eradication of bacteremia due to Campylobacter cinaedi in a human immunodeficiency virus-infected person. Rev Infect Dis. 1991 Nov-Dec;13(6):1066–1068. doi: 10.1093/clinids/13.6.1066. [DOI] [PubMed] [Google Scholar]
  19. Steele T. W., McDermott S. N. The use of membrane filters applied directly to the surface of agar plates for the isolation of Campylobacter jejuni from feces. Pathology. 1984 Jul;16(3):263–265. doi: 10.3109/00313028409068535. [DOI] [PubMed] [Google Scholar]
  20. Tee W., Anderson B. N., Ross B. C., Dwyer B. Atypical campylobacters associated with gastroenteritis. J Clin Microbiol. 1987 Jul;25(7):1248–1252. doi: 10.1128/jcm.25.7.1248-1252.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tenover F. C., Williams S., Gordon K. P., Nolan C., Plorde J. J. Survey of plasmids and resistance factors in Campylobacter jejuni and Campylobacter coli. Antimicrob Agents Chemother. 1985 Jan;27(1):37–41. doi: 10.1128/aac.27.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Totten P. A., Fennell C. L., Tenover F. C., Wezenberg J. M., Perine P. L., Stamm W. E., Holmes K. K. Campylobacter cinaedi (sp. nov.) and Campylobacter fennelliae (sp. nov.): two new Campylobacter species associated with enteric disease in homosexual men. J Infect Dis. 1985 Jan;151(1):131–139. doi: 10.1093/infdis/151.1.131. [DOI] [PubMed] [Google Scholar]
  23. Vandamme P., Falsen E., Pot B., Kersters K., De Ley J. Identification of Campylobacter cinaedi isolated from blood and feces of children and adult females. J Clin Microbiol. 1990 May;28(5):1016–1020. doi: 10.1128/jcm.28.5.1016-1020.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Vandamme P., Falsen E., Rossau R., Hoste B., Segers P., Tytgat R., De Ley J. Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int J Syst Bacteriol. 1991 Jan;41(1):88–103. doi: 10.1099/00207713-41-1-88. [DOI] [PubMed] [Google Scholar]
  25. Wachsmuth I. K., Kiehlbauch J. A., Bopp C. A., Cameron D. N., Strockbine N. A., Wells J. G., Blake P. A. The use of plasmid profiles and nucleic acid probes in epidemiologic investigations of foodborne, diarrheal diseases. Int J Food Microbiol. 1991 Jan;12(1):77–89. doi: 10.1016/0168-1605(91)90049-u. [DOI] [PubMed] [Google Scholar]

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