Abstract
A new differential medium, CHROMagar Candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. The recovery of yeasts on the medium was not significantly different from the recovery on Sabouraud dextrose agar. The identities of 450 green colonies on CHROMagar Candida, presumptively identified as Candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-N-acetylgalactosaminidase. Candida tropicalis also formed distinctive colonies, and other yeasts including Candida (Torulopsis) glabrata, Candida Parapsilosis, Candida Magnoliae, Candida lusitaniae, Candida Famata, Candida kefir, and Saccharomyces cerevisiae were readily distinguished from C. albicans and C. tropicalis isolates. CHROMagar Candida is a very useful medium, and its use will facilitate the study of yeasts associated with dental diseases.
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- Beighton D., Hellyer P. H., Lynch E. J., Heath M. R. Salivary levels of mutans streptococci, lactobacilli, yeasts, and root caries prevalence in non-institutionalized elderly dental patients. Community Dent Oral Epidemiol. 1991 Oct;19(5):302–307. doi: 10.1111/j.1600-0528.1991.tb00172.x. [DOI] [PubMed] [Google Scholar]
- Budtz-Jörgensen E., Stenderup A., Grabowski M. An epidemiologic study of yeasts in elderly denture wearers. Community Dent Oral Epidemiol. 1975 May;3(3):115–119. doi: 10.1111/j.1600-0528.1975.tb00291.x. [DOI] [PubMed] [Google Scholar]
- COSTA S. O., BRANCOCDE L. EVALUATION OF A MOLYBDENUM CULTURE MEDIUM AS SELECTIVE AND DIFFERENTIAL FOR YEASTS. J Pathol Bacteriol. 1964 Apr;87:428–431. doi: 10.1002/path.1700870226. [DOI] [PubMed] [Google Scholar]
- Cumming C. G., Wight C., Blackwell C. L., Wray D. Denture stomatitis in the elderly. Oral Microbiol Immunol. 1990 Apr;5(2):82–85. doi: 10.1111/j.1399-302x.1990.tb00232.x. [DOI] [PubMed] [Google Scholar]
- Kreher J. M., Graser G. N., Handelman S. L., Eisenberg A. D. Oral yeasts, mucosal health, and drug use in an elderly denture-wearing population. Spec Care Dentist. 1991 Nov-Dec;11(6):222–226. doi: 10.1111/j.1754-4505.1991.tb01483.x. [DOI] [PubMed] [Google Scholar]
- Odds F. C., Bernaerts R. CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species. J Clin Microbiol. 1994 Aug;32(8):1923–1929. doi: 10.1128/jcm.32.8.1923-1929.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PAGANO J., LEVIN J. D., TREJO W. Diagnostic medium for differentiation of species of Candida. Antibiot Annu. 1957;5:137–143. [PubMed] [Google Scholar]
- Parvinen T., Larmas M. The relation of stimulated salivary flow rate and pH to Lactobacillus and yeast concentrations in saliva. J Dent Res. 1981 Dec;60(12):1929–1935. doi: 10.1177/00220345810600120201. [DOI] [PubMed] [Google Scholar]
- Perry J. L., Miller G. R. Umbelliferyl-labeled galactosaminide as an aid in identification of Candida albicans. J Clin Microbiol. 1987 Dec;25(12):2424–2425. doi: 10.1128/jcm.25.12.2424-2425.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rousselle P., Freydiere A. M., Couillerot P. J., de Montclos H., Gille Y. Rapid identification of Candida albicans by using Albicans ID and fluoroplate agar plates. J Clin Microbiol. 1994 Dec;32(12):3034–3036. doi: 10.1128/jcm.32.12.3034-3036.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Samaranayake L. P., MacFarlane T. W., Lamey P. J., Ferguson M. M. A comparison of oral rinse and imprint sampling techniques for the detection of yeast, coliform and Staphylococcus aureus carriage in the oral cavity. J Oral Pathol. 1986 Aug;15(7):386–388. doi: 10.1111/j.1600-0714.1986.tb00646.x. [DOI] [PubMed] [Google Scholar]